Spermiogenesis of man, monkey, ram and other mammals as shown by the periodic acid-Schiff technique.
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Guinea pig testicles were extracted with acetic acid; the extract was purified by removing material in consecutive precipitations with 30 per cent saturated ammonium-sulfate, trichloracetic acid, and chloroform. The solution so purified, when administered with complete adjuvants, was highly active in inducing impairment of spermatogenesis in guinea pigs. The activity resisted autoclaving at 15 pounds' pressure for 20 minutes, proteolytic enzymes, and formamide. Anaphylactic shock and cutaneous reaction to the purified homologous extract occurred in guinea pigs sensitized by the extract combined with adjuvants. For the production of aspermatogenesis it was essential to incorporate killed mycobacteria into the water-in-oil emulsion containing the antigen; but anaphylactic sensitization did not require the presence of mycobacteria.
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Exposure to light of bovine spermatozoa suspended in various media results in a progressive decline in metabolic activity followed by the premature death of the cells. The inhibitory and spermicidal effects of visible light resemble a photodynamic action; the photosensitizer is an intrinsic component of the cell.
Rotation of living guinea-pig spermatozoa can be effected by drawing the shaft of a long microneedle of diameter 0.5 to 1.0 micro across the tail; the special characteristics of this rotation demonstrated in the tail a polygonal structure and high resistance of the peripheral fibrils to deformation by pressure of the microneedle.
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