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[Arizona bacteria (salmonella subgenus III), a rarely identified cause of food infections (author's transl)].

A report is given of the infection, described under the clinical picture of enteritis, of a one-year old Turkish child with Salmonella arizonae 61:k:1,5,7 (Arizona 26:29-30) which is largely adapted to sheep. It appears that, outside of the USA reports have not been published on infections with this species of Salmonella. Considering that reports on human infections with Salmonellae of the subgenus III (Arizona bacteria) have rarely been published, it was decided to deal with these Salmonellae in general, especially with their reservoirs, the clinical pathological pictures and the problems associated with the bacteriological diagnosis which is made on the basis of the ready splitting of lactose of numerous species of the Arizona group.

Food Contamination

Pasteurization of salted whole egg inoculated with Arizona or Salmonella.

Recently, Arizona bacteria, close relatives of Salmonella, were recovered from salted whole egg that had been pasteurized by the presently recommended process of 63.3 degrees C (146 degrees F) for 3.5 min. Because of this and the fact that the heat resistance of Arizona in salted whole egg had not been determined, the present study was undertaken. Arizona or Salmonella, grown in Trypticase soy broth supplemented with 2% yeast extract in Fernbach flasks covered with aluminum foil over cotton and guaze at 35 degrees C with shaking at 176 rpm for about 96 h, were found to have the greatest degree of heat resistance. As expected, these cells, when inoculated into salted whole egg at 10(7) cells per ml, survived heating at 63.3 degrees C (146 degrees F) for 3.5 min in a two-phase slug flow heat exchanger. To consistently achieve a 7-log kill of typical Salmonella or Arizona, a treatment of 67 degrees C (152.6 degrees F) for 3.5 min was required. However, if a 7-log kill is mandatory, it remains to be determined whether this process affect the functional properties of this product.

Eggs

[Serological analysis of two complex Salmonella respectively Arizona O-groups (Salmonella O:48 and O:64 - Arizona O:5 and O:29) with the object of combining them into one Salmonella O-group 48 within the Kauffmann-White-schema (author's transl)].

In 1963 KAUFFMANN divided O-group Y (= O:48) of the KAUFFMANN-WHITE-Schema into 3 sub-groups, e.g. 481, 482 - S. dahlem; 481, 482, 483 - S. djakarta; 481, 483, 484 = Citrobacter no. 2624/36. He also recommended the use of the two serotypes S. dahlem and S. djakarta for the preparation of a diagnostic group-serum. At that time, serological relations- especially O-antigenic relations (and even some identities) - between the separate Salmonella and Arizona genera were known, viz. between the Salmonella group 0:48 and the Arizona group O:5. It has now been found that there exist also close serological relations between the Salmonella O-groups 48 and 64 on the one hand and to the corresponding Arizona O-groups 5 and 29 on the other hand, in connection with which the special factor 484 defined by KAUFMANN in the Citrobacter culture no. 2624 embraces the whole Salmonella group O:64 (= Arizona group O:29). Therefore, every Salmonella O:64 strain and Arizona O:29 serotype respectively can be agglutinated with factorserum 484, defined by KAUFFMANN. A special O:64 serum is no longer required. The Salmonella antigen 64 (Ar. 29 or 5.29) has a rule the partial antigens 481, 483, 484 (= Ar. 5,29). Only a few serotypes do not possess factor 483; their components are 481 and 484 (= Ar. 29). The evidence of our findings demonstrates that the Salmonella O-group 64 (= Arizona 29) should be combined with O-group 48 (Ar. 5) and erased from the original Kauffmann-White-Schema and the Arizona Antigenic Schema to avoid a wrong diagnosis.

Absorption

[Structure of the Salmonella from animals, birds and the environment through the period of 1970-1975].

Studied were biochemically and serologically the species of a total of 8738 Salmonella cultures. Most of the investigated strains belonged to subgenus I--95 species; to subgenus II belonged one species (Salmonella sofia); to subgenus III belonged 4 species: Salmonella arizonae 11:b:1, 7; Salmonella arizonae 35:r:z35; Salmonella arizonae 35:z52:1, 5, 7; Salmonella arizonae 58:rz53:z57 of three serologic groups. The attention was focused on more than 20 (new to this country) Salmonellae among which a Salmonella bulgaria species new to the Salmonella genus. Data are given for the origin of the strains, the biochemical and serologic behaviour, the sensitivity to phage O1 and the phage types of Salmonella typhimurium and Salmonella enteritidis.

Animals

Activation of latent Salmonella and Arizona organisms by dehydration of red-eared turtles, Pseudemys scripta-elegans.

Salmonella and Arizona organisms could not be isolated from water samples, feces, and tissues of turtles prior to the experiment. After stressing, Salmonella organisms were isolated from tissues of 7 of 27 stressed turtles and from only 1 of 12 unstressed turtles. Arizona organisms were isolated from 9 of 36 stressed turtles whereas results for all 12 unstressed turtles were negative.

Animals

[Isolation of Salmonella from marine shellfish].

The present paper reports the isolation of Salmonella from oysters and clams encountered in the marine waters of Santos and destined to the human consumption. The results obtained showed the characterization of 41 (59,43%) strains of Salmonella arizonae and 28 (40,57%) of Salmonella typhimurium, by its cultural biochemical and serological properties. The drug resistance of Salmonella to several antibiotics was also investigated by determining the minimal inhibitory concentration in serial agar plate dilutions of the therapeutic agents.

Animals

Enterobacteriaceae isolated from iguanid lizards of west-central Texas.

The prevalence of members of the family Enterobacteriaceae in the intestines of seven species of iguanid lizards native to west-central Texas was determined. Of the 67 lizard specimens examined, 48.7% were infected with Salmonella and 9% were infected with Salmonella arizonae. Two lizard species (Sceloporus olivaceus and Crotaphytus collaris) were shown to have a 100% prevalence of Salmonella.

Animals

Detection and antimicrobial susceptibility patterns of Salmonella enterica subsp. arizonae and Proteus spp. associated with gastrointestinal disease in rescued hedgehogs (Erinaceus europaeus).

Western European hedgehogs (Erinaceus europaeus) are frequently admitted to wildlife rehabilitation centres, where infectious diseases may affect recovery and raise One Health concerns. This study aimed to identify bacterial isolates recovered from hedgehog samples submitted for suspected gastrointestinal infection and to characterise their antimicrobial susceptibility profiles. Five bacterial isolates were analysed using the MicroScan WalkAway Plus® system with the Neg-Urine-Combo 98 panel, and the results were interpreted in accordance with EUCAST guidelines. The identified bacteria included one isolate of Salmonella enterica subsp. arizonae, three isolates of Proteus mirabilis and one isolate of Proteus penneri. The Salmonella enterica subsp. arizonae isolate was susceptible to all antimicrobials for which a valid result was obtained. Proteus spp. isolates were susceptible to cefotaxime, nalidixic acid, ciprofloxacin, levofloxacin, norfloxacin, amikacin, gentamicin, tobramycin, aztreonam, cefoxitin, ceftazidime and fosfomycin. However, resistance was observed to amoxicillin-clavulanic acid, ampicillin, ertapenem, meropenem, trimethoprim-sulfamethoxazole, cefuroxime, piperacillin-tazobactam, colistin and nitrofurantoin, with the latter two showing resistance in all Proteus spp. The Proteus penneri isolate displayed the broadest resistance profile, including resistance to several β-lactams, carbapenems. As expected, all Proteus spp. showed intrinsic non-susceptibility to colistin and nitrofurantoin. Although the Salmonella enterica subsp. arizonae isolate was susceptible to the tested agents, Proteus spp. from hedgehog samples may display relevant antimicrobial resistance (AMR) patterns. Therefore, continuous bacteriological monitoring and antimicrobial susceptibility testing are important in wildlife rehabilitation settings to guide treatment decisions and support One Health surveillance.

Antimicrobial resistance

Antigenic determinants of murein lipoprotein and its exposure at the surface of Enterobacteriaceae.

Murein lipoprotein from the outer membrane of Escherichia coli could be fixed to erythrocytes without pretreatment of the erythrocytes. Passive hemagglutination or immune hemolysis could thus be used as sensitive assays to determine antibodies against lipoprotein. In rabbit antisera prepared against whole cells of E. coli, Salmonella, Arizona, and Shigella antibodies against lipoprotein were present. The respective titers were lowest in encapsulated smooth strains and highest in rough mutants. Antisera against deep rough mutants showed even higher anti-lipoprotein titers than anti-R-lipopolysaccharide titers. Correspondingly,absorption of lipoprotein antibodies with enterobacterial strains was most pronounced with deep rough mutants and lowest with smooth strains. Lipoprotein becomes increasingly an immunogen as well as an antigen the more sugar residues are missing in the lipolysaccharide on the cell surface. In wild-type cells lipoprotein is buried in the outer membrane; its exposure in mutant cells is related to defects at the cell surface.

Animals

Laboratory-acquired infections at the National Animal Disease Center 1960--1976.

Experience with exposure to, or infection with pathogenic agents at the National Animal Disease Center is summarized. A total of 60 laboratory-associated exposures to infectious disease agents were reported. Forty-nine exposures resulted from known accidents, but the other 11 were identified only after the development of clinical or serological manifestations of infection. Eighteen cases of laboratory-acquired infections were reviewed. Brucellosis, the most frequently reported laboratory-acquired infection, accounted for one-half of the cases summarized. Three cases of leptospirosis, two cases of Newcastle disease, two cases of ringworm, and a single infection with Mycobacterium bovis and with Salmonella arizonae were also encountered. The most frequently reported causes of exposure were: auto-inoculation or spray exposure associated with the use of the hypodermic syringe, cuts or lacerations, direct contact with infected animals, and mouth pipetting. Although the infecting event could not be identified in 11 infections, presumptive evidence suggests aerogenic transmission as a probable route of exposure in a number of such cases.

Animal Diseases

[Further studies of salmonella findings in reptiles. IV. Communication (author's transl)].

All together 139 snakes - 134 Coluber jugularis, 3 Natrix natrix and 2 Natrix tessellata were examined for the presence of Salmonella and Arizona bacteriae. From the 139 specimens examined 112 proved to be Salmonella positive. 30 Salmonella respectively Arizona serotypes were isolated, there from 3 new Arizona types were found: Ar. 10a, 10c:27-21, Ar. 10a, 10c:23-31 and Ar. 16:23-31. 41 specimens contained single serotypes, while 51 were double infected, 12 specimens harbored 3 different serotypes of Salmonella, 6 specimens contained four and 2 contained five different serotypes. The result of these examinations present once more the variety of Salmonella species in reptiles.

Animals

[Four new Salmonella species and three serological variants of subgenus III (Arizona) (author's transl)].

Four new strains of Salmonella and three serological variants described in this paper were isolated from free living snakes (Vipera berus L. and Natrix natrix L.) of Northern Germany. All strains belong to the subgenus III of the genus Salmonella. For the first time a representative of subgenus III in the Salmonella group M with the serological formula S. arizonae 28:Z10:Z57 was isolated. 1) S. (6),14:1,v:z (Ar. 7 a,7c:23-31) 2) S. 17:Z10:e,n,X,Z15:Z56 (Ar. 12:27-28-38) 3) S. 21:1,v:Z57 (Ar. 22:23-40a,40c) 4) S. 28:Z10:Z57 (Ar. 35:27-40a,40c) 5) S. 38:(k):Z35:Z56 (Ar. 16:22-21-38) 6) S. 43:1,v:Z56 (Ar. 21:23-38) 7) S. 50:Z10:Z:Z56 (Ar. 9a,9c:27-31-38)

Animals