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[Mutagenic effect of several pesticides on durum spring wheat].

Spraying of spring wheat plants during vegetation with herbicides of 2,4-D group and ethrel increased chromosome aberrations in meiosis 2.8-5, 3.6-4.1 times. The insecticides, chlorophos and phtalophos, in a little higher dosage than it is usually practised increased the spontaneous level of chromosome aberrations 3.1-5.5 times. Phtalophos also induced chlorophyll mutations. These results testify to the necessity of keeping strictly to the established normative doses, because of overdosage is of potential genetic danger to spring wheat crops.

2,4-Dichlorophenoxyacetic Acid

Variation of cadmium concentration in Swedish wheat and barley. An indicator of changes in daily cadmium intake during the 20th century.

In order to evaluate whether or not an increase in daily cadmium intake may be taking place, 75 wheat samples (spring wheat and fall wheat) harvested from the same "nonpolluted'' farm fields between the years 1916 and 1972 were analyzed. The individual scatter was large but a significant (Psmaller than.05) tendency toward increasing concentration with time was evident for fall wheat. A similar tendency was found for spring wheat, through not a statistical significance. This increase may be explained by general air contamination or the cumulative effect of fertilizers. There was a clear covariation in cadmium concentrations of differenc genotypes of wheat and barley harvested in the same year despite yearly variations in average concentration.

Air Pollution

Survey of U.S. wheat for ochratoxin and aflatoxin.

A total of 291 hard red winter wheat samples, 286 hard red spring wheat samples, and 271 soft red winter wheat samples were analyzed for the presecne of ochratoxin and aflatoxin. Samples in all grades came from those collected during crop years 1970-1973 for grade determinations by the Agricultural Marketing Service, U.S. Department of Agriculture. Sensitivity limits of the analytical method as carried out were 1-3 ppb aflatoxin B1 and 15-30 ppb ochratoxin A. No aflatoxin was detected in any sample. Three samples of hard red winter wheat (Grades U.S. No. 4 and 5 and Sample Grade) contained ochratoxin A (trace, 35, and 25 ppb, respectively). Eight of the hard red spring wheats contained ochratoxin A (15-115 PPB); these were in Grades U.S. No. 4 and 5 and Sample Grade.

Aflatoxins

[Biological value of Mironovskaya-808 winter wheat grown using the herbicide simazine].

The biological value of spring wheat Mironovskaya-808 grown with the use of simazine which was introduced into soil prior to appearance of shoots at doses of 0.5 and 1.0 kg/ha has been studied. The organoleptic parameters evaluated by the method of closed testing and physicochemical parameters characterizing the corn quality (protein fat, gluten and ash content, moisture, acidity) of experimental samples did not differ from those of the reference sample. Relative biological value of wheat grown with the use of simazine at a dose of 1.0 kg/ha evaluated by the test-object Tetrahymena pyriformis was slightly higher as compared to the control. The effect of test wheat samples on some parameters of protein metabolism was also studied in male rats (the content of protein and its fractions in blood serum, liver and brain as well as activity of enzymes participating in protein metabolism regulation: aminotransferases of blood serum and liver, catepsins, histidase and tryptophan oxygenase of the liver). According to the data obtained no adverse effect of spring wheat Mironovskaya-808 grown with the use of simazine on the above parameters of protein metabolism was recorded in test animals.

Alanine Transaminase

Chloroplastic proteins of wheat and rye grown at warm and cold-hardening temperatures.

Soluble proteins and membrane polypeptides were separated from chloroplasts isolated intact from a cultivar each of spring wheat, winter wheat, and more freeze-resistant rye, and changes in them associated with cold hardening were detected by means of polyacrylamide gel electrophoresis. No drastic changes in chloroplast membrane polypeptides occurred during growth at low temperatures in the three cultivars. However, subtle changes were evident in the soluble chloroplast protein fraction. In this fraction at least one varietal difference was discernable, yet all cultivars produced a new protein band of lowest mobility during growth at low temperatures. After the preparations were fractionated by Sephadex G-50 all unhardened plant material displayed two peaks in the region of the fraction I protein band, whereas all cold-hardening material displayed one peak. A different band of soluble protein was present only after cold hardening in Kharkov whear and Puma rye, and was not present in extracts from the cold-grown spring wheat.

Chloroplasts

Effect of low temperature stress on the expression of sucrose synthetase in spring and winter wheat plants. Development of a monoclonal antibody against wheat germ sucrose synthetase.

A monoclonal antibody against wheat germ sucrose synthetase is developed and characterized. Its use in studying the effect of cold acclimation on the expression of sucrose synthetase in winter and spring wheat plants is described. The antibody shows cross-reactivity with sucrose synthetase from maize and pea plants, as well as carrot cells. A gradual accumulation of the enzyme as a function of time spent at 2 degrees C is observed in both wheat varieties. In contrast, an initial sharp rise in the mRNA level is observed, which agrees with the previously reported response of maize plants subjected to anaerobic stress.

Antibodies, Monoclonal

Effects of some cereal brans and textured vegetable protein on plasma lipids.

The hypothesis that dietary fiber lowers serum cholesterol was tested in 10 healthy men, 19 to 54 years old, who ate a mixed diet similar to the diets of many American adult males, that contained 16% of calories as protein (70% from animal), 40% as fat (P/S = 0.3), 44% as carbohydrate (9% of calories as sucrose) and 3 g of crude fiber. The energy intake ranged from 2700 to 3500 kcal adjusted to their height and weight. Weight and fitness were held constant. After 30 days of equilibration on the basal diet, they ate 26 g of either soft white wheat bran, corn bran (CB), soybean hulls (SH), textured vegetable protein, or hard red spring wheat bran (HRS) for periods of 28 to 30 days each in no particular sequence. Each fiber was fed to four to six subjects. The dietary fiber contents of soft white wheat bran, CB, SH, and HRS were: 44, 92, 87, and 51%, respectively. Mean daily fecal weight increased (P less than or equal to 0.01) from 72.4 to 144, 68 to 128, and 81 to 151 g when CB, SH, and HRS were fed respectively. No effects were noted with soft white wheat bran or textured vegetable protein. Total plasma cholesterol decreased 12% with HRS (P less than or equal to 0.05) and 14.0% with SH (P less than or equal to 0.05). Low density lipoprotein cholesterol decreased 21% with HRS (P less than or equal to 0.05). High density lipoprotein cholesterol did not change with any of the dietary fiber sources nor did the ratio of high density lipoprotein cholesterol to total cholesterol. Some triglyceride lowering effect was seen with all sources of dietary fiber (P less than or equal to 0.01). There was a significant direct correlation between the area under the oral glucose tolerance curves and the levels of total cholesterol (r = 0.57, P less than or equal to 0.0001) and low density lipoprotein cholesterol (r = 0.49, P less than or equal to 0.0007), and between fasting plasma glucose and triglycerides (r = 0.32, P less than or equal to 0.03). Results were replicated when subjects were fed the same fiber source on two occasions at 2 to 4 month intervals.

Adult

Barley diets with different fat sources have hypocholesterolemic effects in chicks.

Saturated fat is known to elevate serum cholesterol, whereas soluble dietary fiber has a hypocholesterolemic effect. The objective of this study was to compare the effects of barley and wheat diets supplemented with five fat sources on lipid metabolism in chicks. In two separate experiments, broiler chicks were fed isonitrogenous diets containing 23% protein, 11.4% dietary fiber and 10% dietary fat for 17 d. Diets contained 60% either hull-less barley or red spring wheat, with either palm oil, dehydrated egg yolk, butter, tallow or corn oil. Growth, feed efficiency, plasma lipids, liver cholesterol, and fecal fat and dry matter were measured. All chicks fed wheat grew faster and had greater food efficiency than those fed barley. All barley-fed chicks had lower (P less than 0.0001) total plasma cholesterol concentration (3.1 to 4.0 mmol/L) than those fed wheat (6.0 to 11.3 mmol/L). Chicks fed palm oil with wheat had the highest total cholesterol, 11.3 mmol/L. Liver cholesterol concentration was higher (P less than 0.0001) for all wheat-fed chicks (22.8-86.4 mmol/g) compared with those fed barley (6.7 to 12.2 mmol/g). Fecal crude fat was higher (P less than 0.05) for chicks fed barley, and excreta dry matter was lower for barley-fed chicks. Results indicate that the high soluble fiber content of this barley exerts a hypocholesterolemic effect in chicks regardless of dietary fat source, possibly mediated through lowered fat absorption.

Animals

Genetic architecture and evolution of stripe rust resistance uncovered using diverse panels of wheat lines and North American Puccinia striiformis f. sp. tritici isolates.

Screening global wheat germplasm with a diverse collection of pathogen races expands the catalog of novel Yr loci and identifies new sources of broad-spectrum resistance against evolving Pst populations. Newly emerging highly virulent races of Puccinia striiformis f. sp. tritici (Pst) often defeat deployed resistance genes (Yr), highlighting the need for novel sources of durable resistance. A global diversity panel of 377 spring wheat (Triticum aestivum L.) lines was screened for all-stage resistance (ASR) against a panel of diverse 20 Pst isolates at the seedling stage and for adult-plant-stage resistance (APR) against natural mix of field races. Genome-wide association mapping identified 77 unique Yr loci. Of these, 34 overlapped with the previously mapped 1150 Yr loci, confirming the robustness of our GWAS results, while 43 were likely novel. Comparison of the nine adult-plant-stage Yr loci mapped in our study with known APR genes identified only one overlap, with Yr29. Except for Yr29, APR genes Yr18 and Yr36 were detected at low frequencies, indicating that resistance in our panel may arise from less characterized or novel sources. Two wheat lines, lacking widely effective Yr5 and Yr15 alleles, exhibited resistance to all 20 Pst races at the seedling stage and natural field races at the adult stage, suggesting that they may carry novel, broad-spectrum ASR alleles. Wheat improvement had no effect on the frequency of ASR alleles but resulted in a threefold increase in the frequency of APR alleles, suggesting that the latter were subjected to more consistent breeding selection over time. Our findings underscore the value of combined screening of diverse germplasm with diverse pathogen races to identify novel sources of broad-spectrum resistance for breeding stripe rust resistant cultivars.

Triticum

Evaluation of 10 chemicals for aneuploidy induction in the hexaploid wheat assay.

This study was a part of an international project sponsored by the Commission of the European Communities to evaluate the utility of certain bioassays including hexaploid wheat assay to identify potential aneugens. Ten suspect spindle poisons, i.e. colchicine (COL), cadmium chloride (CdCl2), chloral hydrate (CH), diazepam (DIZ), econazole (EZ), hydroquinone (HQ), pyrimethamine (PY), thiabendazole (TB), thimerosal (TM), and vinblastin sulphate (VBL) were tested for their ability to induce green and/or white leaf sectors as indicators of loss or gain of a chromosome respectively, in Neatby's strain of Chinese Spring wheat (2n = 6x = 42). All the chemicals tested in this study, with the exception of CH and HQ yielded positive response.

Aneuploidy

Identification of Novel Sources and Genetic Mapping for Bacterial Leaf Streak Resistance in a Geographically Diverse Panel of Wheat.

Bacterial leaf streak (BLS), caused by Xanthomonas translucens pv. undulosa (Xtu), has recently emerged as a significant threat to wheat production in the Northern Great Plains region of the United States. Deploying resistant cultivars is an economical and practical method of controlling BLS. To identify novel sources of BLS resistance, we screened a set of 355 bread wheat landraces and cultivars representing global diversity for their response to BLS. A wide distribution of seedling responses against BLS was observed, with most genotypes displaying a moderately to highly susceptible response. Notably, we identified 5 resistant and 33 moderately resistant responses. A high-resolution genome-wide association study using 302,524 high-quality single-nucleotide polymorphisms (SNPs) identified 10 significant marker-trait associations (MTAs) on chromosomes 1A, 1D, 3B, 4A, and 5A corresponding to unique genomic regions associated with BLS resistance. Compared with previous studies, four of these genomic regions are likely novel. Of these, MTA 'scaffold15531_2782724' associated with q5A.1 was highly significant (-log10P = 9.39) and exhibited the highest SNP effect (0.35). An association on chromosome 3B validated a previously identified 3B quantitative trait locus (QTL) mapped at approximately 6 Mbp in the hard red spring wheat cultivar 'Boost', and the high-resolution mapping from our study further refined the interval for this QTL. Furthermore, the narrow haplotype blocks reported in this study could be valuable for fine mapping of important regions. The novel resistant sources, along with identified genomic loci and corresponding SNP markers from this study, would be helpful for wheat-breeding programs to enhance BLS resistance.[Formula: see text] Copyright © 2026 The Author(s). This is an open access article distributed under the CC BY 4.0 International license.

BLS

[On the determination of chlorocholine chloride (CCC) in cereals (author's transl)].

For the determination of CCC residues samples of grains and straw of four varieties of spring wheat and oat were extracted with ethanol. CCC was purified by column chromatography with an ion exchanger and alumina as well as by TLC. After its identification by the Rf and a colour reaction on the thin layer plate a colorimetric method with dipicrylamine was used for the quantitative estimation of CCC. The recovery rate amounts to 98+/-5% for wheat grains and straw and to 80 +/- 5% for oat grains. The detection limit is at 0.1 ppm. The amounts of CCC reach in wheat grains 0.3--0.7 ppm, in wheat straw 1--7 ppm and in oat grains 0.9--3.4 ppm in the dry matter. In oat straw CCC could not be detected with this method.

Chlorides