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Results for “Subclinical mastitis”

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At least 19 recordsLinked to original sources
PubMed2026

Long non-coding RNAs link DNA methylation to immune regulatory networks in bovine subclinical mastitis.

Long non-coding RNAs (lncRNAs) are emerging as important regulators of inflammatory and immune signaling, yet their contribution to bovine subclinical mastitis remains poorly defined. Here, we characterized the lncRNA expression landscape associated with disease in milk somatic cells of healthy and subclinical mastitic Vrindavani cattle. We identified 11,403 high-confidence lncRNAs, of which 104 were differentially expressed in subclinical mastitis (adjusted P&#x2009;<&#x2009;0.05; |log2FC| &#x2265; 1), with the vast majority upregulated in mastitic samples. Predicted cis- and trans-associated target analyses identified 637 non-redundant genes, and KEGG analysis identified 8 significantly enriched cis-associated pathways and 152 significantly enriched trans-associated pathways (adjusted P&#x2009;<&#x2009;0.05), predominantly enriched for immune and inflammation-related pathways. These findings prioritized a subset of mastitis-associated lncRNAs for subsequent methylation and interaction-network analyses. A subset of these lncRNAs further overlapped differentially methylated regions (DMRs), suggesting a potential association between lncRNA expression changes and DNA methylation alterations. Integration of lncRNA-miRNA and miRNA-mRNA interactions identified lncRNA-miRNA-mRNA interaction networks involving DMR-associated lncRNAs. Among the prioritized candidates, MSTRG.28878.1 showed overlap with a hypomethylated promoter-associated DMR, increased expression, and multiple connections within the predicted interaction network. Together, these findings identify candidate lncRNAs, methylation-associated loci, and predicted molecular interactions associated with bovine subclinical mastitis and provide a resource for future functional investigation of candidate non-coding RNA-associated mechanisms in disease.

Animals↗
PubMed2026

Whole genome sequence data set of methicillin-resistant Staphylococcus aureus isolated from a milkman associated with cows with subclinical mastitis in Kiruhura district, Uganda.

The whole-genome sequence data set for methicillin-resistant Staphylococcus aureus, which was isolated from a milkman associated with cows with subclinical mastitis in the Kiruhura district of Uganda, is presented here. The assembled genome size was 2822,509 bp, with a 33% GC, 2 Contigs, a Contig N50 of 2818,424, and 1 Contig L50. You can access the genome sequence and related metadata at https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_056782255.1/. This dataset can be used again for resistance gene mapping, comparing genomic analysis, and comprehending genetic diversity among MRSA isolates from Ugandan milkmen.

Antimicrobial-resistant genes Staphylococcus aureu↗
PubMed1977

[Hibitan and Iosan SST in the prevention of subclinical mastitis in cows].

The effect was tested of the disinfection preparations hibitan and iozan SST under field conditions in the course of six months. The animals treated were included in two groups: group I--with 20 cows, and group II--with 39 cows. All animals were free of mastitis-causing agents. The udder of the animals in the first was disinfected with hibitan-super-concentrate (7.5 per cent chlorhexidin gluconate). Prior to milking the udder was washed with a 0.12 per cent solution. After milking the teats were disinfected with a hibitan cream. The udder of the animals of the second group was disinfected with iozan SST (17.6 per cent tensit-iodo-iodide complex). Prior to milking the udder was washed a 0.5 per cent solution, and the teats after milking were disinfected with a 33 per cent iozan SST solution. It was found that the washing of the udder and teats as indicated above in the course of 6 months rules out the outbreak of the infectious subclinical mastitits in cows. It is suggested to adopt the use of the two preparations to prevent mastitis in field and farm practice.

Animals↗
PubMedDate not supplied

Prevalence of bovine subclinical mastitis in Malawi.

A survey comprising 934 cows of a total of 1180 from 22 dairy herds and the examination of bulk milk samples from 273 small-holders was carried out. At the time of sampling all known dairy herds and small-holders in Malawi were examined. Since relative few infections other than Staph. aureus and Str. agalactiae were revealed in the first 12 herds where quartermilk samples were taken composite milk samples (all four quarters into one sample) were taken in the remainder of the herds. The results of the survey are shown in Tables I a, b, c and II a and b. The major problems were infections caused by Str. agalactiae and Staph. aureus. The amount of pathological secretion was low compared to countries with a developed dairy industry such as Denmark.

Animals↗
PubMed1977

[Biochemical and serological studies of the E. coli strains isolated from cows with subclinical mastitis].

Studies were serologically and biochemically a total of 17 strains of Escherichia coli isolated from mastitis-affected cows in the districts of Sofia, Varna, and Plovidiv. Demonstrated were the serogroups 0125, 0111, 086, 025, 026, 055, and 0128, which were pathogenic for humans too. Via the milk they were shown to reach both newborn animals and children and to cause severely expressed colienteritis. All investigated strains attacked glucose and manite, reduced nitrates into nitrites, and gave a positive reaction with methyl red but did not ferment starch, insulin, sucrose, and maltose; neither did they produce hydrogen sulfide and liquefaction of gelatin. They gave a negative Voges-Proscauer reaction. Single strains were negative with regard to lactose and indole, and the strains' activity with regard to sorbite, inosite and adonite varied.

Animals↗
PubMed1979

[Comparative studies of total milk protein and protein fractions in various forms of bovine mastitis].

The content of total protein was assessed in 112 milk samples and of protein fractions--in 106 samples taken from cows suffering from clinical mastitis, subclinical mastitis, secretory disturbances, latent infection, and normal milk. Total protein increased along with the rise of cell content. It increased considerably also in the secretion of cows suffering from clinical mastitis, but the rise in the milk of cows with subclinical mastitis, secretory mastitis and laten infection was insignificant. In all mastitis forms the content of serum albumins and gamma-globulin rose significantly, while alpha-lactoalbumins and beta-lactoglobulin were reduced. These changes in the protein fractions of milk can be used with certainty in the diagnosis of all forms of mastitis.

Animals↗
PubMedDate not supplied

[Microbial etiology of subclinical bovine mastitis in the Santa Fe dairy basin].

A study of 320 samples of mammary quarters from cows in 40 dairy farms around Santa Fe city during 1977-1978 was made. The samples were selected based on clinical examination and California Mastitis Test (GMT). High percentage of subclinical mastitis was found. The etiological agents most frequently isolated were Staphylococcus aureus (54,1%), Streptococcus agalactiae (23.4%) and Pseudomonas aeruginosa (13,2%). A winter increment of S. aureus (48,1%) and P. aeruginosa (3,7% and 22,5%) was observed. But S. agalactiae did not experiment considerable variation (26,8% and 20% throughout the year. The sensitivity to antibiotics of some strains decreased in winter specially in S. aureus and P. aeruginosa (61,5% and 38,9%), and some biochemical properties related with their virulence increased. Penicillin in milk was not detected, but the levels of contamination by non specific inhibitory substances were very high (40%). Besides it was found a great contamination with aerobic sporeforming bacteria in these samples, being Bacillus coagulans (59,1%), B. polymyxa (26,1%) and B. sphaericus (9,5%) the most frequent.

Animals↗
PubMed2026

Genomic Insights into Mammaliicoccus sciuri from Subclinical Bovine Mastitis to Unveil Key Resistance, Virulence, Biofilm and Adaptation Traits.

The Mammaliicoccus sciuri (M. sciuri), is recognized as a reservoir of antimicrobial resistance (AMR) genes, poses challenges in the Indian dairy sector where antibiotic use is poorly regulated. This study aimed to genomically characterize M. sciuri (formerly Staphylococcus sciuri) isolates recovered from subclinical mastitis (SCM) cattle milk. A total of 128 composite (quarter-wise pooled) milk samples were collected from 199 households (HH) across 16 epiunits /villages in four blocks of Chikkaballapur district, Karnataka, India. Of these, 36 milk samples (28.13%, 36/128; 95% CI: 21.06&#x2013;36.46%) were diagnosed with SCM using the California Mastitis Test (CMT) and bacteriological culture yielded 113 isolates (88.28%; 113/128; 95% CI: 81.56&#x2013;92.77%) were phenotypically identified as Staph spp. Through molecular technique PCR targeting the gap gene, two isolates (1.77%; 2/113; 95% CI: 0.49&#x2013;6.22%) from Hosuru and Gattamaranahalli epiunits were confirmed as M. sciuri and both isolates were mecA-positives indicating methicillin resistance. Whole genome sequencing (WGS) identified 36&#x2013;37 resistance genes (mecA and blaZ), conferring resistance to &#x3b2;-lactams, macrolides, fluoroquinolones and aminoglycosides. Horizontal gene transfer (HGT) was evidenced by diverse mobile genetic elements (MGEs) such as SCCmec variants, insertion sequences, transposons (IS3, IS6, IS256, and IS1182) and plasmids (Rep1, Rep13, RepUS5 and RepUS43). Virulence profiling uncovered biofilm-associated genes (ica, bap) and heavy metal resistance operons (ars, cop, znu) suggesting mechanisms for environmental persistence and co-selection of resistance traits. Phylogenetic analysis of 99 global isolates revealed host-and geography-specific clustering with Indian isolates occupying distinct evolutionary niches. These findings highlights its possible role as an AMR reservoir and also in bovine mastitis.

Animals↗
PubMed1978

[Microbiological studies on bacterial spectra of milk samples from healthy udder quarters and from those with increased cell counts and/or conductivity values].

The germ levels of 2,182 milk samples obtained from udder quarters with subclinical mastitis were compared to milk sampled from 2,061 udder quarters with physiological cell counts or conductivity values. Three cattle herds were involved in the test programme. No germ growth was established from 9.5 per cent of all samples taken from udder quarters with increased cell counts and conductivities and from 4.1 per cent of those samples taken from intact udder quarters. Samples taken from udder quarters with subclinical mastitis exhibited the following rises in bacteria, as compared to samples from intact quarters: staphylococci by 3.1 per cent, staphylocci in germ mixtures by 3.0 per cent, CAMP-positive streptococci by 2.2 per cent, alpha-haemolytic CAMP-negative streptococci by 0.8 per cent, anhaemolytic streptococci in germ mixtures by 0.4 per cent, beta-haemolytic streptococci by 0.5 per cent, and Pseudomonas aeruginosa by 1.4 per cent. Other germ species and mixtures exhibited declining trends along with growing subclinical affection of udder quarters. All findings so far obtained in the presence of subclinical mastitis are likely to suggest that 11.4 per cent of detected bacteria were of pathogenicity to udders. However, attempts to localise those 11.4 per cent were unsuccessful, since no significant difference could be calculated by comparison of intact with affected udder quarters. Reference is made in the discussion to primary and secondary germ levels of milk samples and their relevance to the problem and its elucidation.

Animals↗
PubMed1979

Economic losses from and the national research program on mastitis in the United States.

Scientific papers estimating economic losses from mastitis were reviewed. Reduced milk production from cows with subclinical mastitis was responsible for the largest losses. Losses from mastitis in the United States in 1976 were estimated by usable responses to a survey from 33 states representing 9.5 million cows or 86% of the dairy cow population. Losses of milk yields caused by mastitis were 386 kg/cow per yr and losses of discarded milk 62 kg/cow per yr. Annual losses per cow from mastitis were a) reduced milk production, $81.32; b) discarded milk, $12.88; c) cost of veterinary services, $1.97; d) cost of drugs, !3,86; e) increased labor, $2.28; f) decreased sale value, $5.72; g) increased replacement costs, $9.32; h) total, $117.35. For the 11 million cows in the United States 1976 losses from mastitis were $1.294 billion. Mastitis research was supported publicly at 22 locations under 43 projects. Approximately 24 scientist years were involved and $2.7 million of public funds were expended annually. If economic losses from mastitis were reduced 2% per year for 10 yr by research and expenditures for mastitis research remained the same, the benefit to cost ratio from mastitis research would be approximately 9.6 to 1.

Agriculture↗
PubMed1979

[Diagnostic significance of lactose level of milk. 2. Lactose level in first quarter milk samples in various udder disorders].

The behaviour of lactose levels in first quarter milk was studied in the context of a large dairy herd in the presence of various udder health defects. The results were related to other criteria generally used in mastitis diagnosis. The lactose level in first quarter milk was found to be a diagnostic criterion which was properly applicable to detecting and identifying clinical and subclinical mastitis. A limit value of 4.6 per cent has been commonly assumed in literature and proved to be a sound basis for udder health assessment, provided that the given phase of lactation is taken into due consideration (exclusion of cows in the first month post partum as well as of aged milking cows from the seventh month of lactation). Also reported in this paper are results regarding milk lactose and its correlations with other criteria for mastitis diagnosis. A close correlation was found to exist between lactose level and electric conductivity of first quarter milk.

Animals↗
PubMed1979

[Staphylococcus aureus variety hominis in a cattle herd].

A site-linked hominis variety of Staphylococcus aureus was isolated from a cattle herd. The find coincided with accumulated occurrence of clinical mastitis in cows and the affliction of one milker with a nose furuncle. The origin of the strain was not elucidated. The same strain had been isolated throughout three years of observation from clinical and subclinical mastitis as well as from chronic udder affection of cows, but no extraordinary accumulation of clinically manifest mastitis had been observed. The hominis site variety was quite rare among Staphylococcus aureus strains isolated from other cow herds. Enterotoxin formation was recorded from strains of the hominis site variety and from strains which could not be coordinated with any other of the known site varieties and fell under crystal-violet Type A. No enterotoxin formation was recordable from the strains of the bovis variety. The same applied to the group of staphylococci of crystal-violet Type C which could not be coordinated either with any known site variety and which is assumed to have originated from the hominis site variety. The above findings do not support any conclusion as to whether the cows had been infected by the milker or vice versa.

Animals↗
PubMed1979

[Duovac 300--udder health and milking routines. A field experiment (author's transl)].

The effect on udder health and milking routine of two Alfa Laval milking units, the Duovac 300 with a normal (380 mm Hg) and a low (250 mm Hg) vacuum phase and the standard HP 100 unit with only the normal (380 mm Hg) vacuum phase, was investigated on six dairy farms. A total of 197 cows were involved in the study. Each herd was divided into two groups which were as similar as possible in terms of age, stage of parturition and milk yield. One group was milked with the Duovac unit, while the other was milked with the HP 100 units for a test period of 100 days. No differences in cell counts from the cow samples, the presence of subclinical mastitis or clinical condition of the teats were observed between the two test groups. The total milking machine time for the Duovac units was longer than for the HP 100 units. However, the time spent in the normal vacuum phase (380 mm Hg) for the Duovac units was shorter than the total milking machine time for the HP 100 units (380 mm Hg). The Duovac units had the shortest machine stripping time, but the "waiting time" for the milkers (the time between the various operations) was longer. These time studies showed that the number of cows milked per hour by one man using four or five Duovac units was equal to or greater than the number of cows milked per hour by one man using three HP 100 units. It was recommended that no more than three HP 100 units be managed by one man.

Animals↗
PubMed1979

Scanning electron microscopy-aided observations on and therapy of teat canal infections.

An examination of teat canal swabs established that 51 teat canals out of 68 quarters of machine-milked cows were colonized by Staphylococcus aureus. Only 31 of these quarters yielded milk from which S. aureus could be cultured, and 6 out of the 31 produced milk containing somatic cell counts in excess of 500 000/ml. No inhibitory substances could be detected in milk samples 12 h after 10 mg of sodium cloxacillin had been deposited in the test canal on 1-4 successive occasions. Teat canal swabs and milk sample cultures of the same quarters became and remained bacteriologically negative for at least a week after the last treatment. Six quarters, which according to the International Dairy Federation criteria were suffering from subclinical mastitis, became negative after local teat canal therapy. Scanning electron micrographs of one infected teat canal revealed the presence of cocci in depressions and crevices on the epithelial surface, suggesting that such cocci are not always flushed out into milk samples. Teat canal therapy should make a marked contribution to the control of bovine mastitis.

Animals↗
PubMed1979

[Histochemical behavior of succinate dehydrogenase and lactate dehydrogenase as well as ribonucleic acid in the epithelium of lactic ducts and alveoli of cow udder].

The activities of succinate dehydrogenase (SDR) and lactate dehydrogenase (LDH) as well as the ribonucleic acid levels in the epithelium of both the lactic ducts and alveoli of lactic glands were examined in heifers, close to full term, lactating cows, some of them with subclinical mastitis lesions, and udder biopsies of two cows. The activities of SDH and LDH as well as RNA levels were pronounced in the glandular epithelium of alveoli and in the epithelium of the lesser lactic ducts. All three parameters were also pronounced but at lower levels in the cells of the apical stratum of the two-layer epithelium in the greater lactic ducts. Relationships were found to exist between the enzyme activities and RNA levels and the lactation cycle. Their patterns and manifestations were more or less typical of those areas of the lactic gland which were affected by mastitis.

Age Factors↗