Heterogenous and autogenous-tendon transplants. An experimental study of preserved bovine-tendon transplants in dogs and autogenous-tendon transplants in dogs.
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Autogenous patellar tendon grafts were transplanted into the knees of 40 New Zealand White adult rabbits. Grafts were subsequently analyzed for rate of collagen synthesis, collagen content, collagen type, histologic change, and cyanogen bromide cleavage patterns of collagen to closely assess the nature of collagen in tendon grafts up to 2 years from the time of transplantation. Tendon grafts were placed in rabbit knees as free fragments or were attached to synovium. These studies show that tendon grafts, even without vascularization or stress, remain viable after intraarticular transfer. Vascularization produces a trend toward increased collagen synthesis, but statistical analysis suggests that control levels of collagen synthesis continue after tendon transfers into rabbit knees. Cyanogen bromide cleavage peptides showed appropriate collagen formed by unstressed autogenous tendon transplants removed from rabbit knees up to 2 years from transplantation. All tendon grafts degenerated initially, but began to form histologically healthy looking connective tissue by 18 to 24 weeks after transplantation. Overall, the results are encouraging with regard to the fate of intraarticular tendon grafts.
Alessandro Codavilla (1861-1912), Director of the famous Rizzoli Institute of Bologna, was one of the leading figures in orthopedic surgery at the turn of the century. The surgical transplantation of tendons and muscles was being widely practiced in most of the leading centers at that time with very variable success. Codavilla is credited by many of his contemporaries including Bielsalski, Mayer and Steindler for his classic and fundamental studies in the physiological aspects of tendon transfer. Published in 1899, this article generally established the physiologic concepts of modern tendon transplantation surgery.
Flexor tendon grafting in two stages, using silastic rods to prepare a new bed, is well known. In our opinion four indications are important: first -- loss of tendon and gliding surface due to infection or complex injuries; second -- lesions of the digital nerves; third -- defects of the skin and fourth -- replantation of amputated digits. We are convinced that two stage tendon grafting is a helpful concept for achieving reasonable functional results in special cases and complex injuries.
The authors describe three methods for making tendon transplants by using chemical sterilizing and preserving means having no unfavorable effect on their biological and plasty properties. The methods are simple, reliable and fairly suitable for a wide supply of clinical institutions with tendon transplants.
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Staged flexor tendon repair utilizing temporary silastic rod implants has proved to be an effective method for restoring the grip function of severely injuried hands. Since its inauguration by CARROLL and BASSETT in 1958 this method has been successfully practiced by numerous hand surgeons. In this review the patho-physiological principles of the development of the so-called "pseudo tendon sheath" are described. The various findings as far as the formation of the new tendon sheath is concerned, are presented. The theories about incorporation of the subsequent graft and its revascularization are described. Following a detailed description of the surgical procedure our own results with 272 silastic rod implants in 184 patients are presented. Because of the kind of patients selected for the procedure, excellent results were not obtained. However, in 62% of cases results were graded "good" and in 23%, "satisfactory", while 15% were "poor". It seems to be justified to utilize two-stage flexor tendon repair for restoring grip function in severely injured hands in spite of the considerably high technical requirements, the prolonged course of rehabilitation and consequently increased cost of treatment.
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Authors have investigated the ultrastructural morphology of transformation of homologous human tendon transplants. Tenoblasts and mast cells were found next to collagenous fibres originating in the transplant and disintegrating in the voluminous ground substance. The formation of new collagen tissue was of different grades; areas of only a few fibres and areas with oriented fibres were both present. Twenty months after implantation parts characterised by apparently mature structure were found as well as abundant new fibre formation and mast cells.
Four autologous tendon grafting techniques, bipedicle, pedicle and free grafts of the deep digital flexor tendon and free grafts from the lateral digital extensor tendon were described. The vascularization and remodelling of the transplant and the reactions in the donor, as well as the recipient tendon, were studied by means of microangiographic and histochemical methods. The results indicated that regardless of the technique used the grafts were revascularized and replaced by a highly orientated and organized tendon. The use of free grafts may therefore be worthwhile in treatment of severe tendon lesions in valuable racehorses.
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