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At least 19 recordsLinked to original sources
PubMed1975

Accumulation and excretion of neutral lipids in the metacercaria of Leucochloridiomorpha constantiae (Trematoda) maintained in vitro.

Histochemical and thin-layer chromatographic analyses were made on neutral lipids in Leucochloridiomorpha constantiae (Trematoda) metacercariae incubated in non-nutrient Locke's solution at 37.5 or 42 C for 24 hr. As determined by Oil Red O staining, lipid droplets accumulate mainly in the intestine of incubated worms. Attempts to distinguish specific neutral lipid fraction of metacercariae obtained directly from snails is free sterol. During incubation, metacercariae accumulate triglycerides and to a lesser extent cholesterol esters, and excrete free sterol into the medium.

Animals↗
PubMed1978

In vivo and in vitro excystation of Zygocotyle lunata (Trematoda) metacercariae and histochemical observations on the cyst.

Encysted metacercariae of Zygocotyle lunata (Trematoda) excyst within 2 hr postexposure in the lower ileum of the domestic chick. Optimal in vitro excystation of this species occurs following pretreatment of the cyst for 15 min in 1% acidified pepsin, treatment in 0.02 M sodium dithionite (a reductant) for 1 to 2 min and then 2 hr treatment in an excystation medium containing 1% sodium glycocholate plus 1% trypsin in Earle's BSS adjusted to pH 8.8 with tris and maintained at 41 C. The cyst of this species is a dome-shaped hemisphere containing an inner and outer wall. The outer wall contains mainly acid mucopolysaccharides, whereas the inner wall is mainly proteinaceous. The cyst contains a ventral lid which only was visualized during excystation.

Animals↗
PubMed1975

[Contribution to the study of Microphallidae Travassos 1920 (trematoda). XXXII. Microphallus breviatus n. sp., a species with an abbreviated evolutive cycle from a Mediterranean pond in the Languedoc].

Contribution to the study of Microphallidae Travassos, 1920 (Trematoda). XXXII. - Microphallus breviatus n. sp., a short life-cycle species of a mediterranean pond of Languedoc. The whole larval life-cycle of M. breviatus takes place in one host, Hydrobia ventrosa (Montagu), Mollusc Hydrobiidae. Hepato-pancreatic sporocysts produce morphologically altered xiphidio-cercariae which become encysted metacercariae in the sporocyts themselves. This species is defined in the genus by the anatomic characteristics of its cercariae and metacercariae, allied with its uncommon biology including two hosts only.

France↗
PubMed1975

[Evolutive cycle of Paratimonia gobii, Prevot and Bartoli 1967 (trematoda-monorchiidea)].

The cycle of Paratimonia gobii PREVOT & BARTOLLI, 1967 (Trematoda-Monorchiidae), a parasite of Pomatoschistus microps (Teleostei gobiidae), has been discovered and carried out experimentally. The mollusc, first intermediary host, is Abra ovata (Lamellibranchiata, Scrobiculariidae). The gymnocephalous cercaria leaves the mollusc, then sucked in by the respiratory stream, becomes encysted in the inhaling siphon of another Abra. The accumulation of metacercaria results in the autonomy of this siphon and it is this cut-off organ which is becoming part of the alimentary chain of the final host allows the continuation of the cycle.

Animals↗
PubMed1977

Histochemical and thin layer chromatographic analyses of neutral lipids in metracercarial and adult Cotylurus sp. (Trematoda: Strigeidae).

Histochemical and thin layer chromatographic studies were made on neutral lipids of Cotylurus sp. (Trematoda) metacercariae obtained from Physa heterostropha snails and from adults grown in the upper ileum of the domestic chick. Oil Red O staining demonstrated neutral lipids in the intestinal lumina, eggs and vitellaria of adults, and the excretory system of metarcercariae. As determined by TLC, excysted metacercariae incubated in a non-nutrient salt solution excreted free fatty acids, sterols, and sterol esters into the medium. TLC analysis detected free sterols, free fatty acids and sterol esters in metacercariae and adullts, and triglycerides detected in adults were not found in metacercariae.

Animals↗
PubMed1975

Nasitrema attenuata (Trematoda) infection of Tursiops truncatus and its potentialities as an aetiological agent of chronic pulmonary lesions.

Two cases of Nasitrema sp (Trematoda) infection of the bottlenosed dolphin, Tursiops truncatus, are reported and N. attenuata is briefly redescribed. The adults of the trematode occurring in the nasal sinuses and posterior nasal passage of the dolphins are considered as practically harmless for the host but thier eggs, aspirated deep into the bronchial tree, may initiate a foreign-body of inflammatory reaction in the lungs and continuous aspiration of such eggs may provoke a chronic pneumonia condition.

Animals↗
PubMed1992

Study on the surface morphology of the developmental stages of the liver fluke, Opisthorchis viverrini (Trematoda: Opisthorchiidae).

The external morphology of some developmental stages of the fluke Opisthorchis viverrini (Trematoda: Opisthorchiidae), parasitizing humans in Southeast Asia was studied for the first time using electron microscopy. The surface structure of the egg, as well as the rediae, cercaria, metacercaria, and adult found in naturally infected hosts from Laos are described herein and their morphological characteristics discussed.

Animals↗
PubMed1979

Thin-layer chromatographic analysis of phospholipids in Echinostoma revolutum (Trematoda) adults.

Thin-layer chromatographic analyses were made of phospholipids extracted from adult Echinostoma revolutum (Trematoda) and from the nonnutrient medium in which worms were maintained. Identity of phospholipids was based on comparison with authentic lipid standards and on specific chemical detection tests. The most abundant phospholipids in the extract were phosphatidyl choline, phosphatidyl ethanolamine and phosphatidyl serine; lesser amounts of cerebrosides and lysophosphatidyl choline were detected also. Phospholipids detected in the incubation medium were essentially as described for extracts.

Animals↗
PubMed1977

Effect of anoxic incubation on the oxygen uptake of Isoparorchis hypselobagri (Digenea: Trematoda).

Anoxic incubation for varying periods of Isoparorchis hypselobagri from the swim bladder of the catfish Wallago attu, led to an accumulation of oxygen debt on the part of the parasite. With increasing length of anoxic incubation, there is a corresponding increase in post-anaerobic oxygen uptake, and the longer the period of incubation is, the greater is the time taken to return to a normal rate of oxygen uptake.

Air Sacs↗
PubMed1975

[Influence of CO2 and pH on the attachment response of the cercaria of Diplostomum spathaceum (trematoda) (author's transl)].

1. The attachment of the cercaria to artificial substrates (offered via dialyzing membranes) in definite media was investigated under conditions of variable pH and [CO2]. 2. A decrease of the pH of the substrate releases only attachments in CO2 containing media and consequently acts via CO2 systems of the medium. 3. As effective components of CO2 systems, dissolved CO2 + H2CO3 are confirmed. 4. The sensitivity of the reaction on gradients of the CO2 partial pressure (in solution) could be established by offering substrates with lowered pH in CO2 containing media. Thus, by raising the CO2 partial pressure from ca. 0,04% to 0,15% maximal fixation rates were obtained (Fig. 3). 5. The carboanhydrase inhibitor acetazolamide, when added to the medium, had no direct influence on the CO2 receptors.

Acetazolamide↗
PubMed1975

Gas content of swim bladder of Wallago attu and oxygen consumption in Isoparorchis hypselobagri (Trematoda).

The swim bladder gas of Wallago attu contains O2 (22-58 mm Hg) and the presence or absence of Isoparorchis hypselobagri does not influence the O2 content of the swim bladder. Glucose increases the O2 consumption of I. hypselobagri by 50%. With the passage of in vitro culture time, the rate of O2 consumption gradually decreases to the extent of 64% on the 40th day. The optimal temperature for O2 consumption is 30 degrees C beyond which the rise in temperature is detrimental to the fish trematode as manifested by the decrease in their O2 consumption.

Air Sacs↗
PubMed1976

Qualitative analysis of substances excreted by Isoparorchis hypselobagri (Trematoda) during aerobic in vitro culture.

Isoparorchis hypselobagri from the swim bladder of the catfish, Wallago attu was aerobically cultured in vitro. The dialyzed incubate was microchemically analysed for either individual or group of compounds excreted by the parasite. Amino acids, amines, amides, ammonia, aldehyde, glycine, alpha-alanine, tyrosine, alpha-aminocarboxylic acids, haemoglobin, nitrogen, reducing materials, and lactic, pyruvic, succinic, propionic and acetic acids were found in the incubate, but acetaldehyde, formaldehyde, O-dioxo and oxomethylene, and formic acid were not detected.

Aerobiosis↗