Inhibition of rat cholinesterase by tritolyl phosphates.
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Three contaminants were identified during drug screening of postmortem blood samples which had been stored in glass bottles with a black rubber seal. Two of these contaminants, cyanoethyl dimethyldithiocarbamate and N-phenyl-2-naphthylamine, were found to come from the rubber seal of some bottles. The third contaminant was not a single compound but rather a mixture of aryl phosphates with a composition very similar to technical grade tritolyl phosphate. The origin of these phosphates is at present unknown.
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Tri-p-ethylphenyl phosphate is unique amongst the organophosphorus compounds which produce neurotoxic effects in not being an inhibitor of cholinesterase. The dysfunction it produces is also marked by some unusual features. Thus it produces a characteristic high-stepping gait which develops at varying periods after intramuscular injection but more regularly following oral administration. A careful comparison of the character, onset and development of the effects of diisopropyl phosphorofluoridate (dyflos), tri-o-cresyl phosphate and tri-p-ethylphenyl phosphate has illustrated the differences between the two former substances and tri-p-ethylphenyl phosphate. It has also confirmed a previous suggestion that this substance acts in a different manner from the other two, a suggestion supported by the histological evidence.
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