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Results for “Verticillium wilt”

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PubMed1978

Influence of soil pH and calcium nutrition on resistance of alfalfa to bacterial and verticillium wilt.

Alfalfa plants of a resistant, a susceptible and a highly susceptible strains were grown in unlimed soil at pH 5.8 and in limed one at pH 6.9 and inoculated by the pathogens of vascular wilt, Corynebacterium insidiosum and Verticillium albo-atrum. Two types of liming were performed: 1) before inoculation and 2) after inoculation. Liming of the soil led to an increase in number of resistant plants. In susceptible plants the external symptoms of disease on the plant tops were delayed or alleviated. This phenomen was more conspicuous with Verticillium wilt than with bacterial wilt. The favourable effect of liming was less distinct in resistant strains than in susceptible ones. For an increase in resistance, post-infection liming of the soil was more effective in the case of bacterial wilt, while pre-infection liming provided the best results in the Verticillium wilt. The nitrogen content in the dry matter of roots from plants grown in limed soil was higher by more than a quarter as compared to roots from plants growing in unlimed soil.

Calcium↗

PubMed1979

The cell-wall phosphatase of cotton (Gossypium) is inhibited by kelthane.

Kelthane [4,4'-dichloro-alpha-(trichloromethyl)benzhydrol] was previously shown to decrease the limited tolerance of susceptible varieties of cotton (Gossypium) to Verticillium wilt. Kelthane was shown in the present study to inhibit the cell-wall p-nitrophenyl phosphatase of cotton. In view of information already establishing the cell wall as a primary site of action of Verticillium wilt, the data are interpreted as suggesting an as yet undefined interaction between Kelthane, cell-wall phosphatase and verticillium-resistance mechanisms of the cell wall.

4-Nitrophenylphosphatase↗

PubMedDate not supplied

[Multiple forms of pectin trans-eliminase from Verticillium dahliae Klebahn -- cotton wilt agent].

From the culture liquid filtrate of Verticillium dahliae--cotton wilt agent--pectin trans-eliminase (EC) was isolated. The enzyme was isolated and examined, using ultrafiltration, gel filtration, ion exchange chromatography, isoelectrofocusing, and electrophoresis. The fungus was found capable to produce several forms of pectin trans-eliminase that differed in their molecular weight, charge, synthesis and release regulation, substrate action (position of bonding breakdowns in the pectin polymer molecule). Pectin trans-eliminase activity was also detected in cell walls of the fungal mycelium. Possible origin of multiple forms of the enzyme is discussed.

Chromatography, Affinity↗

PubMed2026

Genome-Wide Characterization of the ZIP Transporter Family in Sea Island Cotton (Gossypium barbadense L.) and Expression Profiling Under Heavy Metal and Pathogen Stresses.

G. barbadense represents an indispensable germplasm resource for high-quality textile fiber and disease resistance; nevertheless, systematic information regarding its ZRT/IRT-like protein (ZIP) gene family remains limited. Here, a total of 46 GbZIP genes were identified across the G. barbadense genome. Comprehensive bioinformatic investigations revealed uneven chromosomal distribution and confirmed that segmental/whole-genome duplications, supplemented by localized tandem duplications, drove family expansion. Members clustered within the same phylogenetic clades shared conserved motif organization and gene architecture, while promoter regions harbored abundant cis-acting elements associated with phytohormone and stress signaling. Transcriptome profiling indicated distinct expression patterns across vegetative/reproductive tissues, fiber and ovule developmental stages, and diverse abiotic stress conditions (cold, hot, drought, and salt). Quantitative Real-Time PCR (qRT-PCR) further validated that several GbZIP candidates exhibited temporal expression variations upon exposure to cadmium toxicity, V. dahliae infection, and combined Cd-V. dahliae stress. Specifically, GbZIP13, GbZIP18, GbZIP27, and GbZIP36 displayed prominent broad-spectrum responses to all three stress conditions, whereas GbZIP16, GbZIP29, and GbZIP30 showed stress-specific regulatory divergence. Overall, this study aims to systematically analyze the evolutionary characteristics and expression patterns of the GbZIP family, and to specifically evaluate the response differences under Cd stress, V. dahliae stress, and combined stress, in order to identify potential key candidate genes.

Gossypium barbadense↗