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The effect of supplementation with fresh browse of Ziziphus mauritiana or Combretum aculeatum on feed intake, nitrogen utilization and growth of grazing range sheep.

The influence of 0, 60 or 120 min access to a grove of either Ziziphus mauritiana (Ziziphus) or Combretum aculeatum (Combretum) on forage intake and on the digestibility, growth and excretion of nutrients was determined using 40 Oudah rams grazing for 7 h/day on poor-quality dry season pasture in the Sahel. The effects of browse species and browsing duration (0, 30, 60 or 120 min) on the ruminal ammonia content were also evaluated using 8 mature fistulated rams. Ziziphus increased both total digestible organic matter and total dry matter intakes per (kg live weight)0.75 without decreasing herbage intake, whereas 60 or 120 min access to Combretum reduced herbage intake by 6.3% and 4.2%, respectively. The digestibility of the diet decreased (p < 0.05) with the duration of access to the groves. Despite the provision of more nitrogen (N) than in the control diet, ruminal NH3-N decreased 24 h after browsing commenced. The reduced live weight gain of sheep browsing Combretum may indicate more deleterious compounds in Combretum than in Ziziphus. Ziziphus appears to have more potential than Combretum to increase sheep production in low-input crop/livestock systems.

Animal Feed↗

Mucilages and polysaccharides in Ziziphus species (Rhamnaceae): localization, composition and physiological roles during drought-stress.

The drought-tolerant tree species Ziziphus mauritiana Lamk. and Z. rotundifolia Lamk. were shown to have similar high mucilage concentrations (7-10% dry weight) in their leaves, with large numbers of mucilage-containing cells in the upper epidermis and extracellular mucilage-containing cavities in the leaf veins and stem cortex. The main sugar constituents of the water-soluble mucilage extract were rhamnose, glucose and galactose. During drought-stress in two independent studies, foliar mucilage content was unaffected in both species, but glucose and starch contents declined significantly in crude mucilage extracts from droughted leaves. Enzymatic hydrolysis of the mucilage extract using alpha-amylase and amyloglucosidase released glucose, indicating that a mucilage-associated water-soluble glucan, with alpha-1,4- and alpha-1,6-linkages, may exist which was extracted together with the mucilage. From the current data, it is not possible to localize the glucan to determine whether or not it is associated with mucilage-containing cells. Data from pressure-volume analyses of drought-stressed and control leaves showed that, in line with their similar mucilage contents, the relative leaf capacitance isotherm (change in relative water content per unit change in water potential) was similar in both species. During drought-stress, reduced relative capacitance resulted from osmotic adjustment and decreased wall elasticity. Data suggest that in Ziziphus leaves, intracellular mucilages play no part in buffering leaf water status during progressive drought. In Ziziphus species, growing in environments with erratic rainfall, the primary role of foliar mucilage and glucans, rather than as hydraulic capacitors, may be as sources for the remobilization of solutes for osmotic adjustment, thus enabling more effective water uptake and assimilate redistribution into roots and stems prior to defoliation as the drought-stress intensified.

Adaptation, Physiological↗

Identification of the R2R3-MYB gene family in wild jujube (Ziziphus jujuba var. spinosa) and analysis of its expression under drought stress.

BACKGROUND: R2R3-MYB gene family serves as a pivotal regulatory factor in plant growth, development, and responses to environmental stresses. To investigate its function in the drought stress response of wild jujube (Ziziphus jujuba Mill. var. spinosa), a typical eco-economic forest species, this study performed genome-wide identification and relevant analyses of R2R3-MYB genes. RESULTS: A total of 91 R2R3-MYB genes (designated as ZjMYB1 to ZjMYB91) were identified, which were unevenly distributed across 12 chromosomes. These genes mainly encode hydrophilic and unstable proteins, 97.8% of which are localized in the nucleus. Phylogenetic analysis classified these genes into 25 clades, showing evolutionary conservation and species-specific divergence with the R2R3-MYB protein family. The expansion of the ZjMYB family is mainly characterized by segmental duplication, and all duplicated gene pairs have undergone purifying selection. ZjMYBs are widely involved in plant growth and development as well as abiotic stress responses, with the highest expression level particularly in leaf tissues; a total of 13 genes were specifically annotated as water deficit response-related genes in drought stress and abscisic acid (ABA) signaling pathways. Integrating the above analyses together with transcriptome data and qRT-PCR validation results revealed that ZjMYB5, ZjMYB53, ZjMYB57 and ZjMYB85 function as core drought-responsive genes, which display both tissue-specific and time-dependent expression patterns under drought stress. CONCLUSIONS: This study systematically elucidated the functional characteristics and regulatory network of the R2R3-MYB gene family in wild jujube, providing critical genetic resources and a theoretical basis for dissecting the molecular mechanisms underlying drought tolerance in wild jujube and breeding drought-resistant cultivars.

Ziziphus↗

Simultaneous determination of saponins and fatty acids in Ziziphus jujuba (Suanzaoren) by high performance liquid chromatography-evaporative light scattering detection and pressurized liquid extraction.

The seed of Ziziphus jujube Mill. var. spinosa (Bunge) Hu ex H. F. Chou, Suanzaoren in Chinese, is one of commonly used Chinese medicines. Saponins and fatty oil contains several fatty acids in Suanzaoren are responsible for its therapeutic activities. In this study, a new HPLC coupled with evaporative light scattering detection (ELSD) and pressurized liquid extraction (PLE) method was developed for the simultaneous quantitative determination of 11 major components of 2 saponins and 9 fatty acids, namely jujuboside A, jujuboside B, lauric acid, myristic acid, palmitic acid, palmitoleic acid, stearic acid, oleic acid, linoleic acid, arachidic acid and docosanoic acid in Suanzaoren. Simultaneous separation of these eleven compounds was achieved on a C18 analytical column. The mobile phase consisted of (A) 0.1% aqueous acetic acid and (B) methanol with 0.1% acetic acid using a gradient elution. The drift tube temperature of ELSD was set at 75 degrees C, and nitrogen flow-rate was 1.8l/min. All calibration curves showed good linearity (r(2)>0.9955) within test ranges. This method showed good reproducibility for the quantification of these eleven components in Suanzaoren with intra- and inter-day variations of less than 3.41 and 4.37%, respectively. The validated method was successfully applied to quantify 11 investigated components in nine commercial samples of Suanzaoren.

Calibration↗

Activity-guided fractionation of the seeds of Ziziphus jujuba using a cyclooxygenase-2 inhibitory assay.

Bioactivity-guided fractionation of petroleum ether- and EtOAc-soluble extracts of the seeds of Ziziphus jujuba using a cyclooxygenase-2 assay as a monitor indicated that the triglyceride, 1,3-di-O-[9(Z)-octadecenoyl]-2-O-[9(Z),12(Z)-octadecadienoyl]glycerol (3), and a fatty acid mixture of linoleic, oleic and stearic acids, were the major active components. A new pentacyclic lupane-type triterpene derivative, 3-O-[9(Z)-octadecenoyl]betulinic acid (1), and betulinic acid (2) were also isolated and identified. All isolates as well as pure linoleic, oleic and stearic acids were evaluated for their inhibitory effects against both cyclooxygenases-1 (COX-1) and -2 (COX-2).

Cyclooxygenase 1↗

The ethnobotany of Christ's Thorn Jujube (Ziziphus spina-christi) in Israel.

This article surveys the ethnobotany of Ziziphus spina-christi (L.) Desf. in the Middle East from various aspects: historical, religious, philological, literary, linguistic, as well as pharmacological, among Muslims, Jews, and Christians. It is suggested that this is the only tree species considered "holy" by Muslims (all the individuals of the species are sanctified by religion) in addition to its status as "sacred tree " (particular trees which are venerated due to historical or magical events related to them, regardless of their botanical identity) in the Middle East. It has also a special status as "blessed tree" among the Druze.

Adult↗

Ceanothane- and lupane-type triterpenes with antiplasmodial and antimycobacterial activities from Ziziphus cambodiana.

One new and eight known ceanothane- and lupane-type triterpenes were isolated from the root bark of Ziziphus cambodiana PIERRE (Rhamnaceae). Based on spectral analyses, the structure of the new compound was elucidated as 3-O-(4-hydroxy-3-methoxybenzoyl)ceanothic acid (3-O-vanillylceanothic acid) (1), while the known compounds were identified as lupeol (2), betulinaldehyde (3), betulinic acid (4), 2-O-E-p-coumaroyl alphitolic acid (5), alphitolic acid (6), zizyberanalic acid (7), zizyberenalic acid (8) and ceanothic acid (9). Compounds 1, 5 and 8 exhibited significant in vitro antiplasmodial activity against the parasite Plasmodium falciparum, with inhibitory concentration (IC50) values of 3.7, 0.9 and 3.0 microg/ml, respectively. Compounds 1 and 3-8 showed antimycobacterial activity against Mycobacterium tuberculosis with respective MIC values of 25, 25, 25, 12.5, 50, 50 and 100 microg/ml.

Animals↗

[Optimization study on extraction technology of the seed of Ziziphus jujuba var. spinosa by orthogonal design with multi-targets].

OBJECTIVE: To optimize extraction technology of the seed of Ziziphus jujuba var. spinosa with the targets of the total saponin, total jujuboside A and B and total flavonoids. METHOD: In the method of one-way and orthogonal tests, ethanol concentration, amount of ethanol, extraction time and extraction times were the factors in orthogonal test, and each factor with three levels. RESULT: Ethanol concentration and extraction times had significant effect on all the targets, other factors should be selected in accordance with production practice. CONCLUSION: The best extraction technology is to extract for three times with 8 fold ethanol solution (60%), and 1.5 h each time.

Drugs, Chinese Herbal↗

Physiological and morphological adaptations of the fruit tree Ziziphus rotundifolia in response to progressive drought stress.

The physiological basis of drought resistance in Ziziphus rotundifolia Lamk., which is an important, multipurpose fruit tree of the northwest Indian arid zone, was investigated in a greenhouse experiment. Three irrigation regimes were imposed over a 34-day period: an irrigation treatment, a gradual drought stress treatment (50% of water supplied in the irrigation treatment) and a rapid drought stress treatment (no irrigation). Changes in gas exchange, water relations, carbon isotope composition and solute concentrations of leaves, stems and roots were determined. The differential rate of stress development in the two drought treatments did not result in markedly different physiological responses, but merely affected the time at which they were expressed. The initial response to decreasing soil water content was reduced stomatal conductance, effectively maintaining predawn leaf water potential (Psi(leaf)), controlling water loss and increasing intrinsic water-use efficiency, while optimizing carbon gain during drought. Carbon isotope composition (delta13C) of leaf tissue sap provided a more sensitive indicator of changes in short-term water-use efficiency than delta13C of bulk leaf tissue. As drought developed, osmotic potential at full turgor decreased and total solute concentrations increased in leaves, indicating osmotic adjustment. Decreases in leaf starch concentrations and concomitant increases in hexose sugars and sucrose suggested a shift in carbon partitioning in favor of soluble carbohydrates. In severely drought-stressed leaves, high leaf nitrate reductase activities were paralleled by increases in proline concentration, suggesting an osmoprotective role for proline. As water deficit increased, carbon was remobilized from leaves and preferentially redistributed to stems and roots, and leaves were shed, resulting in reduced whole-plant transpiration and enforced dormancy. Thus, Z. rotundifolia showed a range of responses to different drought intensities indicating a high degree of plasticity in response to water deficits.

Disasters↗

Antimicrobial activities of ziziphus abyssinica and berchemia discolor.

Aqueous, petroleum-ether, chloroform and dichloromethane extracts of both the barks and leaves of Ziziphus abyssinica and Berchemia discolor were tested for activity against Staphylococcus aureus, Escherichia coli and Candida albicans using the hole plate diffusion and the test tube dilution methods. The aqueous extracts showed significant activity against S. aureus and C. albicans.

Candida albicans↗

[Effects of seeds, leaves and fruits of Ziziphus spinosa and jujuboside A on central nervous system function].

The seeds and leaves of Ziziphus Spinosa exert a similar inhibiting effect on central nervous system function, while the fruits have a synergism with pentobarbitol sodium and thiopental sodium on prolongation of sleep and sedation, also decreases coordinated action. Jujuboside A exerts no inhibiting effect, but a synergism with phenylalanine on central nervous system function.

Animals↗

Fatty acids and carotenes in some ber (Ziziphus jujuba Mill) varieties.

Several jujube varieties from the southeast of Spain were analyzed for fatty acid and carotene contents. Triglycerides having medium-chain fatty acids were most abundant in all samples. The main fatty acids were 12:0 (18.3 +/- 9.97), 10:0 (12.5 +/- 19.0), 18:2n6 (9.27 +/- 7.26), 16:1n7 (8.50 +/- 5.77), 16:0 (7.25 +/- 4.35), and 18:1n9 (5.34 +/- 2.52) on total saponifiable oil. The fruits yield 1.33 +/- 0.17 g/100 g saponifiable oil on a dry weight basis. Fatty acid profiles of fruits were found to be influenced by their developmental stage. Multivariable data analyses show that the samples could be grouped on the basis of their fatty acid content. Carotenes were found to be in good agreement with other fruits, varying from 4.12 to 5.98 mg/100 g on a dry weight basis. The contribution to vitamin value reach a mdium of 38 microg RE/100 g on a fresh weight basis.

Carotenoids↗

[Application of biogas fermentation residue in Ziziphus jujuba cultivation].

With field experiment, this paper studied the effects of applying biogas fermentation residue (dregs and slurry) on jujube growth, its fruit quality, and soil fertility. The results showed that biogas fermentation residue could enhance the disease-resistance of jujube plant and its fruit, and improve fruit quality and soil fertility. Compared with applying chemical fertilizers (the control), biogas fermentation residue increased the contents of jujube fruit coarse fiber, vitamin C, amino acids, Fe and P by 27.69%, 24.85%, 19.81%, 10.89% and 5.26%, and of soil organic carbon, total nitrogen and mineral nitrogen by 42.65%, 37.61% and 35.26%, respectively. The soil pH was decreased from 8.75 to 8.21. Biogas fermentation residue could also increase the amount of soil microorganisms. The microbial biomass-C and biomass-N were 59.44% and 56.06% higher than the control, respectively. It was suggested that biogas fermentation residue application could bring better economic and environmental benefits for Z. jujuba cultivation, and also, provide a new approach for no-pollution production of jujube.

Biomass↗

Anxiolytic effect of seed of Ziziphus jujuba in mouse models of anxiety.

The aim of the present study was to investigate the ethanolic extract of Semen Ziziphi jujuba (SZJE) induced anxiolytic effect. The SZJE was orally administered to male ICR mice, at 0.5, 1.0 and 2. 0 g/kg, 30 min before the behavioral evaluation in the black and white test (BWT) and elevated plus maze (EPM). The SZJE at the dosage 0.5-2.0 g/kg increased the first time entry, total changes and times spent in the white chamber of the BWT. The SZJE at the dosage 0.5-1.0 g/kg increased the percentage of time-spent and the percentage of arm entries in the open arms of the EPM and decreased the percentage of time-spent and the percentage of arm entries in the closed arms of the EPM. Furthermore, the SZJE at the dosage of 1. 0 g/kg prolonged the hexobarbital-induced sleeping time in mice and decreased the locomotor activity in rats. These results suggested that SZJE possessed anxiolytic effect at lower dose and sedative effect at higher dose.

Animals↗

Preharvest mycobial population of Indian jujube fruits (Ziziphus mauritiana Lamk.) and their implications in postharvest pathogenesis.

A mycological survey was carried out on the fruit surface of Indian jujube during its growth period (before harvest). A total of 32 microfungi belonging to 20 genera were isolated by surface washing technique. Artificial inoculations with the isolated myco-propagules were also performed in mature jujube fruits and most of them were able to cause rot of varying severity. However, Monodictys castaneae, Eupenicillium lapidosum, Paecilomyces fumosoroseus, Trichurus spiralis and Acremonium spp., although present on the fruit surface, were not able to cause rot, indicating lack of specific macerating enzymes necessary for postharvest pathogenesis.

Fruit↗