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Results for “adventitious root”

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PubMed2026

The CsTBH-CsROP2 Module Regulates Waterlogging Tolerance via Auxin-Mediated Adventitious Root Formation in Cucumber.

Cucumber (Cucumis sativus L.) requires frequent irrigation due to its shallow root system and high transpiration rate of the aboveground parts. However, it is also prone to waterlogging damage. Therefore, understanding its response to waterlogging is crucial for breeding waterlogging-tolerant varieties. Although Rho of Plants GTPases play well-established roles in regulating development and stress signalling, their functions in plant adaptation to waterlogging stress has yet to be fully elucidated. Here, we identified nine CsROP genes in the cucumber genome, which exhibit evolutionary diversification but retain conserved functional domains. Functional analysis revealed that CsROP2 acts as a negative regulator of adventitious root formation. It modulates auxin accumulation in hypocotyl vascular bundles, thereby suppressing adventitious root development and enhancing waterlogging sensitivity. The HD-Zip I transcription factor CsTBH directly binds the CsROP2 promoter and activates its expression. Our study uncovers a CsTBH-CsROP2 module that governs adventitious rooting and waterlogging tolerance by modulating auxin homeostasis. These findings provide new insights into the crosstalk between developmental programmes and stress signalling pathways and offer potential genetic targets for improving stress resilience in cucumber and other crops.

CsROP2↗

PubMed1978

Production of adventitious root primordia on hypocotyls of castor bean seedling, infected with Agrobacterium tumefaciens.

The development of sterile secondary tumours on hypocotyls of castor bean seedlings, inoculated with any of the ten isolates of Agrobacterium tumefaciens tested, were observed below the site of the primary tumours. Histopathological studies performed in the present work indicate that the observed secondary tumours were adventitious root primordia and not the ordinary type of secondary tumours. According to the available literature such findings are reported here for the first time. All the tested isolates of the present study were also able to initiate well defined roots in conjunction with crown gall (teratomas) on Bryophyllum crenata and Kalanchoe marmorata, but not in tomato.

Ricinus communis↗

PubMed2026

MdWRKY75 interacts with MdWOX11 to modulate root growth under salt stress in apple.

The root system is pivotal for plant development, enabling both vegetative growth and tolerance to abiotic stresses like salinity. However, the molecular mechanisms governing root adaptive development in response to salt stress remain poorly understood in apple (Malus domestica Borkh.). In this study, we identified the salt stress-responsive WRKY transcription factor MdWRKY75. Overexpression of MdWRKY75 in transgenic apple negatively regulates adventitious root (AR) formation and salt stress tolerance, whereas reducing MdWRKY75 expression yields the opposite phenotype. Moreover, MdWRKY75 directly binds to the promoter of MdSAUR15 (SMALL AUXIN UP RNA15) and transcriptionally represses the expression of MdSAUR15, which, when overexpressed, promotes AR formation and enhances salt stress tolerance. We further demonstrated that MdWRKY75 interacts with MdWOX11, a WUSCHEL-related homeobox (WOX) transcription factor, both in vitro and in vivo. MdWOX11 expression is upregulated and enhances AR formation under salt stress. Additionally, MdWOX11 reduces the binding of MdWRKY75 to the MdSAUR15 promoter, and alleviates the MdWRKY75-mediated inhibitory effect on MdSAUR15 expression. Collectively, our study provides a MdWOX11-MdWRKY75-MdSAUR15 module regulating root adaptation in response to salt stress in apple.

Malus↗

PubMed1976

Mitotic activity in rice seedlings germinating under oxygen deficiency.

Rice seedlings germinating in oxygen-deficient environments do not manifest visual evidence of seminal or adventitious root growth or normal leaf growth, the coleoptile being the only seedling structure to emerge under these environmental conditions. Microscopic evidence, however, shows that mitotic activity occurs in the different tissues comprising the shoots of the seedlings germinating under oxygen deficiency. Thus evidence is presented here which shows that mitotic activity in rice seedlings germinating in oxygen-deficient environments is not confined solely to the emerging coleoptiles of the germinating seedlings.

Anaerobiosis↗

PubMed1977

A scanning electron microscopic study of IAA-induced tumors in bean (Phaseolus vulgaris L.) embryos.

A scanning electron microscopic study of indole-3-acetic acid (IAA) induced tumour in the hypocotyl region of bean embryos shows a distinct morphological, structural and topographical change from the non-treated bean embryos. The IAA-induced tumour surface, in the hypocotyl region, shows distinct cell enlargement, some cellular proliferation in the parenchymatous tissue, total destruction of the epidermis and stomata and some variation in trichome structures. In dole-3-acetic acid inhibits the normal growth of the epicotyl, and, as age progresses, adventitious roots appear all over the surface. When IAA-depletion occurs, epicotyl growth resumes, which indicates that this tumour formation in bean embryos is an IAA-dependent tumour.

Indoleacetic Acids↗

PubMed1979

The morphology of the vertical and transverse intrinsic musculature of the tongue in the 15-week human fetus.

The attachments, courses and interrelationships of the transverse and vertical intrinsic muscle masses of the tongue were examined in 28 fifteen-week fetal specimens. Observations were made from 30-micron sections cut through the tongue in one of the three standard planes of section. Both sets of muscululature are qualitatively well-developed by this time period in fetal life. The transverse fibers were found to occupy the entire length of the tongue. They attach to the lamina propria of the lateral aspect of the body of the tongue and, in the root, to perimysial and adventitial connective tissue. In addition, some fibers were observed to be confluent with the mm. palatoglossus, tonsilloglossus and pharyngis superior. Medially, transverse fibers were found for the most part to terminate in the dense ventral aspect of the median septum. Vertical fibers are present from a point slightly posterior to the tip of the tongue to the level of the foramen cecum, beyond which they become sparse. All vertical fibers attach superiorly to the dorsal lamina propria. In the free part of the body, their ventral attachment, likewise, is to lamina propria. In the middle part of the tongue and, to a greater extent, in the root (as the inferior and lateral free surface decreases) these fibers attach in either the fascial plane underlying the transverse component or to the perimysium of longitudinally-running muscle bundles.

Connective Tissue↗

PubMed1977

Organogenesis in in vitro cultures of embryonic shoots of Abies balsamea (balsam fir).

Embryonic shoots of 15- to 20-year-old Abies balsamea (balsam fir) trees were soaked in (a) water for 15 min or 24 hr and (b) water with 1000 mg per 1 indolebutyric acid (IBA), N-dimethylaminosuccinamic acid (Alar-85), or 1-phenyl-3-methyl-5-pyrazolone (PPZ), singly or in combination with 100 mg per 1 caffeic acid, for 15 min. After the soaking, the embryonic shoots were transferred to a nutrient medium. Nonsoaked (control) embryonic shoots elongated and often formed a basal callus but never showed organogenesis. The soaked embryonic shoots formed new apical buds, with or without bud scales, adventitious dwarf needles or shoots, and root- and embryo-like structures. One of the embryos germinated and formed an irregular shoot. No differences were found between the various soak treatments, except that the 15-min water soak was ineffective. The 24-hr water soak was as effective as the 15-min growth regulator treatments.

Butyrates↗

PubMed2026

Development of an Efficient Regeneration and Agrobacterium-Mediated Transformation Protocol for Hosta 'Light Star' Using the RUBY Reporter Gene.

Hosta plantaginea is a perennial shade-tolerant herb of the Liliaceae family, with high ornamental and urban greening value. Hosta 'Light Star' is a newly developed ornamental cultivar with yellow-margined leaves and lilac flowers, but no efficient in vitro regeneration or genetic transformation system has been established for this cultivar to date. In this study, we established a highly efficient in vitro regeneration system for Hosta 'Light Star,' and developed an Agrobacterium-mediated genetic transformation protocol using the RUBY visual reporter gene for non-invasive screening of positive transformants. The optimal callus induction medium was MS + 2 mg/L 6-BA + 0.3 mg/L NAA + 0.05 mg/L 2, 4-D, with a callus induction rate of 53.33% for leaf explants (the optimal explant for sterile seedlings). The optimal adventitious bud proliferation medium was MS + 2 mg/L 6-BA + 0.1 mg/L NAA, with a proliferation coefficient of 5.87. The optimal rooting medium was 1/2 MS + 0.5 mg/L NAA + 0.5 mg/L IBA, with a 100% rooting rate. The optimal transplant substrate was perlite:vermiculite = 2:1, with a 100% transplant survival rate after acclimatization. For Agrobacterium-mediated transformation, the optimal infection parameters were as follows: Agrobacterium suspension OD600 = 0.6, infection time of 10 min, and 200 μM acetosyringone; the optimal selection conditions were 300 mg/L cefotaxime for bacteriostasis and 30 mg/L hygromycin for transformant screening. The final stable transformation efficiency was 2.50% (95% CI 1.23-3.77%), with an escape rate of 16.13%. Transgenic plants showed distinct purplish-red coloration in roots, stems, and leaves, with significantly higher betacyanin accumulation than wild-type plants (p < 0.05). Stable integration and expression of the RUBY gene were confirmed by PCR, RT-PCR, and RT-qPCR. This study establishes the first efficient regeneration and Agrobacterium-mediated transformation system for Hosta 'Light Star,' and validates the feasibility of the RUBY reporter gene as a visual marker for Hosta transformation. This system provides a solid technical platform for functional genomic studies, CRISPR/Cas9-mediated gene editing, and molecular breeding of ornamental traits in Hosta.

Transformation, Genetic↗

PubMedDate not supplied

[Osseous echinococcosis. Apropos of 6 anatomo-clinical observations].

Concerning these six cases, the authors recall the rarity of osseous localization of Hydatidiform disease, isolated as a rule, arising in the adult and preferentially involving the ilium, spinal column, femur, humerus and scapula. Being slowly progressive, the condition is revealed by pain, swelling and above all spontaneous fractures (long bones), or signs of root or medullary compression (spinal column), followed sometimes by fistula formation. Radiological diagnosis is often difficult with an osteolytic appearance without appreciable reactional osteogenesis. As for laboratory investigations, they are only of value in orientation; immunofluorescence being the weakest. The extent of osseous lysis frequently demands extensive surgery. From the anatomical standpoint, the hydatid variety of Echinococcosis has a specific intra-osseous development: beginning in the spongy bone, the architecture is intermediary between the hydatid appearance and the alveolar variety. Some cases however comprise a unilocular appearance with a suspicion of adventitial reaction. Extension into the soft parts frequently adopts a macroscopic appearance identical with that of visceral forms. Bony lysis is dependant on osteoclastic resorption or foci of necrosis while reactional osteogenesis is reduced. Medullary cellular reactions are determinant in the anatomical progress: osteitis or histiocyte and plasmodial reaction to the cuticle fragments.

Adult↗