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Biomedical subjects

A A Bialasiewicz

Publications and source records attributed to A A Bialasiewicz.

106 records · Page 6Linked to original sources
PubMed1985

Evaluation of plasmids in tetracycline resistant strains of Neisseria gonorrhoeae and Ureaplasma urealyticum in a case of severe urethritis.

A young man who acquired gonococcal urethritis in the Far East was not cured by repeated i.v. treatment with a broad-spectrum penicillin. Cultures of pre- and post-treatment urethral specimens grew tetracycline-resistant penicillinase-producing Neisseria gonorrhoeae (PPNG) and tetracycline-resistant Ureaplasma urealyticum. The patient was successfully treated with erythromycin, to which both isolates were sensitive. The strain of N. gonorrhoeae carried a novel plasmid of 10.5 Md molecular mass in addition to plasmids previously observed in this organism. The strain of U. urealyticum carried two distinct plasmids, one with mass 4.9 Md and the other one with mass 8.1 Md. This report demonstrates that ureaplasmas can contain plasmids and raises the question if tetracycline resistance is controlled by plasmids or by the chromosome.

Adult↗
PubMed1985

[Corneal symptoms in keratoconjunctivitis caused by Chlamydia].

Between December 1983 and June 1984 280 patients with keratoconjunctivitis had a laboratory work-up for Chlamydia trachomatis infections. Serologic or culture tests produced positive results in 114 cases. Of these, 3 were neonatal blennorrheas and 111 chlamydial keratoconjunctivitides in adults. Corneal involvement was seen in 57 patients. Diffuse punctate keratopathy was noted frequently. Stromal infiltrates were sometimes found in the limbal area distributed evenly over the cornea. Corneal scars were diagnosed in 11 patients and pannus vasculosus in 2. The clinical range of corneal symptoms in chlamydial infections is illustrated with reference to 4 patients.

Adult↗
PubMed1985

[Determination of resistance and antibiotic therapy in bacterial eye diseases of childhood].

From June 1982 to December 1983 children aged between 3 days and 5 years (average 2-1/2 years) with bacterial conjunctivitis, blennorrhea or perforated corneal ulcers were examined microbiologically. The results of 30 antibiotic susceptibility tests were compared with those of adults. Prevalence of Escherichia coli (6%) and Staphylococcus aureus haemolyticus (17%) was observed; Candida species was not found in the young children. Pediatric diseases revealed themselves to be more pronounced in general. The authors diagnosed 3 chlamydial blennorrheas in newborn aged 7, 8 and 10 days. Mixed infections were found significantly less often in children (21%) than in adults (64%). The authors point out the need for epidemiologic antibiotic susceptibility studies to facilitate decision making for ad hoc therapeutic measures that are sometimes indicated in endophthalmitis or severe blennorrhea. The possible complications of antibiotic polypragmasia and the use of fixed combinations are discussed.

Anti-Bacterial Agents↗
PubMed1985

Immunoreactive parathyroid hormone is present in subretinal fluids of the human eye.

(44-68) human Parathyroid hormone (hPTH) was studied in subretinal fluids (SRF) of 20 human eyes with rhegmatogenous retinal detachments. A (44-68) hPTH carboxyl mid-regional radioimmunoassay was used. Depending on the extent and the duration of the retinal detachments, (44-68) hPTH ranged between normal serum levels (ca. 100-300 pg/ml) up to excessive amounts of 4000 pg/ml with longstanding disease and proliferative vitreoretinopathy. Aqueous humors (AH) of one patient with bilateral iridocyclitis and complicated cataract ranged at 90-132 pg/ml (44-68) hPTH, whereas a control population undergoing surgery for senile cataracts had no detectable (44-68) hPTH in AH. The AH of one diseased eye of heterochromia complicata Fuchs showed excessive amounts of more than 4000 pg/ml (44-68) hPTH.

Adult↗
PubMed1984

The importance and frequency of mixed infections with Chlamydia trachomatis and mycoplasmas in acute gonococcal urethritis.

Chlamydia trachomatis, Ureaplasma urealyticum and Mycoplasma hominis are frequently involved in gonococcal urethritis. We investigated 143 male White and Black patients with gonococcal urethritis (average age 22,5 years). Of these 29% had coexisting Chlam. trachomatis infection, 34% U. urealyticum infection and 13% Mycoplasma hominis infection. Conventional penicillin therapy did not affect Chlam. trachomatis, U. urealyticum or Mycoplasma hominis, which persisted in the lower urogenital tract, causing a so-called 'post-gonococcal urethritis.' Additional therapy with tetracycline or erythromycin was successful in most cases.

Acute Disease↗
PubMed1984

[Evaluation of infectious, inflammatory external eye diseases].

Over a period of 4 months the authors observed 240 outpatients with 302 "red eyes." Oculogenital strains of Chlamydia trachomatis were diagnosed in 12.5% of these cases. Bacterial monoinfections were found in 13.9%, coinfections in 63.9%. The remaining 9.7% included Candida sp. (4%) and Herpes I (1.4%) infections, while 4.3% remained unidentified. Allergic conjunctivitis and "dry eye" syndromes were not considered separately in this study. Specific antibiotic therapy improved the clinical condition due to "saprophytic" bacterial coinfections, indicating the pathogenic importance of this type of infection.

Adolescent↗
PubMed1983

Immunomodulating properties and tissue distribution of aromatic retinoids in the immunologically responsive albino rabbit eye.

The effects of retinoid Ro 10-9359 no normal albino rabbit eyes and antigen-induced intra-ocular inflammations were investigated. The distribution pattern of intravenously applied 3H--Ro 10-9359 correlated well with the sites of pharmacological action. Whereas immunologically naive rabbits showed a uveal uptake of 0.164 ng/g wet wt. tissue when 100 micrograms of Ro 10-9359 was administered intravenously, accumulation may amount up to 17.46 ng/g in secondary ocular immune responses. Ro 10-9359 accumulated markedly during secondary stimulation in the uvea, preauricular lymph nodes and the spleen. The chemotactic peptide NForm-Leu-Leu-Phe used to incite a hypopyon attracted Ro 10-9359 into the anterior chamber in vivo. This study indicates that the aromatic retinoid Ro 10-9359 is able to alter certain immune responses and may be involved in intercellular communication during secondary immune responses in the albino rabbit eye.

Animals↗
PubMed1983

[Massive blenorrhea in a newborn infant caused by a Chlamydia trachomatis subtype].

An 8-day-old baby girl was found to be suffering from massive blennorrhoea caused by a strain of Chlamydia trachomatis. Differential diagnosis included possible bacterial (e.g. Pseudomonas, Staphylococci and Haemophilus sp.) and viral causes of the disease. A 10-day systemic therapy with erythromycin 50 mg/kg/body weight is sufficient to prevent possible complications such as pneumonia. The parents also have to be treated for this disease.

Chlamydia trachomatis↗
PubMed1981

Immunological features of psoriasis. Effects of Ro-109359, concanavalin A, pokeweed mitogen, and methotrexate on cultivated lymphocytes.

Isolated peripheral mononuclear cells of psoriasis patients with different disease characteristics, e. g. head-localised, quiescent guttata, confluent active widespread and erythrodermic, were cultured in a modified Mishell-Dutton system. Using the plaque-forming cell (PFC) assay, single cell antibody formation was studied, and class distribution monitored, adding pokeweed mitogen (PWM), concanavalin A (ConA), methotrexate (MTX) or Ro-109359/31, as well as autologous sera to the culture. PFC-estimation vs. sheep red blood cells (SRBC) and burro red blood cells (BRBC) revealed a distinct suppression of primarily IgG-PFC in some of the PWM-treated patients' cultures; ConA maximally reduced PFC by only 50%, compared to 100% for normal immune cells. Ro-109359/31 reduced mainly IgG-PFC in the co-cultures with PWM or autologous sera. MTX resulted in a reduction of IgG-PFC and IgM-PFC equally. The results were compared with cultured immune cells from normal individuals. The two antigenically different indicators, the partial abolition of ConA induced suppression, broad-based immunoglobulin elevation in the sera, and the mainly IgG-formation hint at the role of polyclonal B-cell activation in the perpetuation of psoriasis, which can be specifically reduced by Ro-109359/31. Suppressor cell dysfunctions remain to be discussed.

Antibody Formation↗
PubMed1980

[Disorders of immune regulation in psoriasis].

Immune abnormalities in psoriasis vulgaris are described. Anti-stratum corneum antibodies deposited with C3 have been found in patients with psoriasis. A lot of other immune abnormalities identified in psoriasis: increased serum IgG and IgA levels, lowered DNCB-sensitization, alteration in polymorphnuclear leukocytes (PMN) function, decreased number of T cells, lack of response to concanavalin A (ConA) and an increase in the polyclonal B cell activation. the authors believe that the immunoregulatory defect is due to the disorder of the skin.

Autoantibodies↗
PubMed1979

Binding of bovine parathyroid hormone to surface receptors of cultured B-lymphocytes.

Binding of parathyroid hormone onto B-lymphocytes is detected by the utilization of the labelled antibody membrane assay. The amount of parathyroid hormone bound to the receptor sites was depending on the quantity of cells in the incubation milieu. Each cell line showed typical characteristics in time course of parathyroid hormone binding and maximal receptor capacity. Fragmentation of intact parathyroid hormone, also varying with the cell line tested, was very rapid, even at 24 degrees C. Within 20 min most of the cell lines destroyed 20% of the native hormone in the incubation mixture, indicating a fragmentation rate of up to 2.25 ng/min at 37 degrees C. Bmax and KD for the different lymphocytes was 5.3--19 . 10(11) M and 1.8--18,5 . 10(11) M, respectively. These values are in the range of reported plasma concentrations and may therefore represent more physiological values for the capacity and affinity of membrane receptors.

Animals↗
PubMed1978

Autoantibodies to parathyroid hormone receptor.

Autoantibodies which block the binding of parathyroid hormone to membrane receptors for the hormone were detected in the sera (especially in the IgG fraction) of 49 out of 50 uraemic patients with secondary hyperparathyroidism (patients with high levels of C-regional parathyroid hormone). These antibodies are species-specific. Their presence in the serum in unaffected by dialysis. Inhibition of binding appears to be related to the rise in C-regional parathyroid-hormone levels and the duration of uraemia. The production of cyclic adenosine monophosphate by parathyroid-hormone-stimulated adenyl cyclase was reduced by the blocking antibodies. The findings show that secondary hyperparathyrodism in uraemia is another example of a receptor-antibody disease, but it is not known whether the antibodies act by modifying the affinity of the receptors for the hormone or by reducing the concentration of receptors available.

Animals↗
PubMedDate not supplied

Parathyroid hormone binding to epithelium of the human lens.

Cell homogenates from human lens epithelium express in binding site for Parathyroid hormone. Kinetic data reveal an affinity/dissociation constant to Kd 17.5 x 10(-11) M and a maximal binding of Bmax 15 x 10(11) M. These data are comparable to previously tested tissues as lymphocytes or renal plasma membranes. No differences can be observed for the association characteristics between senile and juvenile lenticular epithelial cells.

Adult↗