Biomedical subjects
Publications and source records attributed to A A Turdyev.
The influence of a tortoise spleen extract (TSE) on lipids of liver endoplasmic reticulum of irradiated (8 Gy) mice has been studied. The administration of TSE immediately after irradiation changes the content of lipid fractions in microsomal membranes preventing the accretion of cholesterol, nonesterified fatty acids, and triacylglycerol. The preparation under study exerts an antioxidant effect.
The administration of a spleen extract of Testudo horsfieldi to mice exposed to lethal radiation of 8 Gy was shown to increase the survival rate and to stimulate haemopoiesis and formation of endocolonies in the spleen. The gel filtration method was used to obtain an active fraction which was subjected to chromatography on cation exchanger to give two more fractions. Active was the fraction with basic components.
A study was made of a phenomenal radioresistance of Testudo horsfieldi. The authors described the features of the postirradiation death at different seasons. A threshold number of the affected systems in the turtles was one order lower than that of mammals. A positive correlation was found between specific affection of the systems and the degree of their radioresistance.
Phase changes in the content of biomembrane lipids and in the rate of their peroxidation were observed in liver microsomes of Testudo horsfieldi irradiated with a dose of 129.10(-1) C/kg. It is suggested that the decrease in the formation of peroxidation products of a diene series at the initial stage of radiation sickness are conditioned by the decrease in the activity of the key enzyme systems which regulate biosynthesis of polyunsaturated fatty acids, while the increase in the sphingomyelin level activates the lipid-dependent enzymes which repair the injured membranes.
It was shown on mice exposed to 8 Gy radiation that a spleen extract from T. horsfieldi has a high therapeutic effect. Bone marrow cellularity is restored as early as 8 days following irradiation and injection of the extract; the survival rate is 71.2% against 4.2 in the control.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
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A study was made of the effect of a drug prepared from a spleen extract of Testudo horsfieldi on energy metabolism and lipid composition of liver mitochondria of irradiated mice (2.06 X 10(-1) C/kg). It was shown that the decompensated low-energy state of liver mitochondria of the exposed mice was changed into the state of the compensated low-energy shift: the physicochemical properties of lipid microenvironment of mitochondria proteins were partially restored.
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CM-5 fraction of tortoise spleen extract injected after irradiation in dose CD50/30 (540 R) raises a survival of mice to 73.4%. The number of endocolonies of spleen increases to 4.0 in experiment against 0.3 in control and the average life of experimental animals increases to 12.7 days against 8.8 in control. In vitro it has been found a considerable increase of RNA-synthetic activity of bone marrow cells stimulated by CM-5 fraction in post-irradiation period. The dose alteration factor for CM-5 fraction is 1.46 according to CD50/30 standard at survival test.