PubMed Health⌕ Search

Biomedical subjects

A Artola

Publications and source records attributed to A Artola.

At least 19 recordsLinked to original sources
PubMed2005

Diabetes mellitus concomitantly facilitates the induction of long-term depression and inhibits that of long-term potentiation in hippocampus.

Memory impairments, which occur regularly across species as a result of ageing, disease (such as diabetes mellitus) and psychological insults, constitute a useful area for investigating the neurobiological basis of learning and memory. Previous studies in rats found that induction of diabetes (with streptozotocin, STZ) impairs long-term potentiation (LTP) but enhances long-term depression (LTD) induced by high- (HFS) and low-frequency stimulations (LFS), respectively. Using a pairing protocol under whole-cell recording conditions to induce synaptic plasticity at Schaffer collateral synapses in hippocampal CA1 slices, we show that LTD and LTP have similar magnitudes in diabetic and age-matched control rats. But, in diabetic animals, LTD is induced at more polarized and LTP more depolarized membrane potentials (V(ms)) compared with controls: diabetes produces a 10 mV leftward shift in the threshold for LTD induction and 10 mV rightward shift in the LTD-LTP crossover point of the voltage-response curve for synaptic plasticity. Prior repeated short-term potentiations or LTP are known to similarly, though reversibly, lower the threshold for LTD induction and raise that for LTP induction. Thus, diabetes- and activity-dependent modulation of synaptic plasticity (referred to as metaplasticity) display similar phenomenologies. In addition, compared with naïve synapses, prior induction of LTP produces a 10 mV leftward shift in Vms for inducing subsequent LTD in control but not in diabetic rats. This could indicate that diabetes acts on synaptic plasticity through mechanisms involved in metaplasticity. Persistent facilitation of LTD and inhibition of LTP may contribute to learning and memory impairments associated with diabetes mellitus.

Animals↗
PubMed2004

Wear behaviour of the pair Ti-6Al-4V-UHMWPE of acrylic bone cements containing different radiopaque agents.

The objective of this study was to improve the wear behaviour of acrylic bone cements by substituting the conventional inorganic radiopaque agents (BaSO(4), ZrO(2)) for different iodinated radiopaque monomers which can co-polymerize with the methyl methacrylate monomer, MMA. To this aim, the wear behaviour of the pair Ti-6Al-4V-UHMWPE (ultra high molecular weight polyethylene) was studied in the absence and in the presence of cement particles (the third body).

Alloys↗
PubMed2004

Propagation of fatigue cracks in acrylic bone cements containing different radiopaque agents.

In this work three iodine-containing monomers were proposed as new radiopaque agents for acrylic bone cements. In previous studies the addition of iodine-containing methacrylate monomers provided a statistically significant increase in tensile stress, fracture toughness and ductility, with respect to the barium sulphate (BaSO4)-containing cement. However, since fatigue resistance is one of the main properties required to ensure a good long-term performance of permanent prostheses, it is important to compare the fatigue properties of these new bone cement formulations with the radiolucent and BaSO4-containing bone cements. Because the acrylic cements have initial cracks, fatigue crack propagation studies were performed. It can be observed that these acrylic cements followed the Paris-Erdogan model. The results showed that the addition of some organic radiopacifiers (DISMA, TIBMA) increased the fatigue crack propagation resistance as compared to the radiolucent cement, being similar to the BaSO4-containing cement. The radiolucent cement showed a low crack propagation resistance.

Complex Mixtures↗
PubMed2003

A radiopaque polymeric matrix for acrylic bone cements.

As part of the search for an alternative to inorganic radiopaque agents, this work studies the possibility of modifying bone cement formulations by incorporating a radiopaque monomer, that is, 4-iodophenol methacrylate (IPMA), in the liquid phase. The monomer was synthesized in the laboratory, and cements were prepared by the standard method. The influence on the different cement characteristics of various monomer concentrations was studied. It was seen that the setting time decreased as the percentage of monomer increased. The radiopacity attained in the 15 vol.% IPMA formulations was about the same as that for a cement containing 10 wt.% barium sulphate. Dynamic and static mechanical properties were measured. The materials did not show significant differences in the glass transition temperature. However, static mechanical properties showed enhanced compressive strength, tensile strength, and elastic modulus with respect to conventional cements formulated with barium sulphate. Histological studies showed a good response of muscular tissue to implanted specimens.

Barium Sulfate↗
PubMed2003

Elimination of barium sulphate from acrylic bone cements. Use of two iodine-containing monomers.

The aim of this work is to present a new approach to joint arthroplasty failure related to bone cement mantle. As barium sulphate is considered one of the main causes of mechanical weakness in the cement, we substituted this inorganic radiopacifier of the solid component for radiopaque monomers in the liquid component. We obtained two different cements, one containing 5 vol% 2-[2',3',5'-triiodobenzoyl] ethyl methacrylate (TIBMA) and the other containing 3,5-diiodine salicylic methacrylate (DISMA). In both cases, the mechanical properties of these new cements were better than those of the barium sulphate-containing cement. The radiopacity obtained was comparable to that of the aforementioned cement and all the samples showed good biocompatibility.

Barium Sulfate↗
PubMed2003

Increased spike broadening and slow afterhyperpolarization in CA1 pyramidal cells of streptozotocin-induced diabetic rats.

Diabetes mellitus is associated with impairments of cognitive function both in humans and animal models. In diabetic rats cognitive deficits are related to alterations in activity-dependent synaptic plasticity in the hippocampus. Many similarities with the pathophysiology of normal brain aging have been noted, and the view emerges that the effects of diabetes on the brain are best described as "accelerated brain aging."In the present study we examined whether CA1 pyramidal neurons from streptozotocin-induced diabetic rats display an increased slow afterhyperpolarization, often considered as a hallmark of neuronal aging. We found no differences in resting membrane potential, input resistance, membrane time-constant, and action potential amplitude and duration between CA1 pyramidal neurons from streptozotocin-induced diabetic and age-matched control rats. During a train of action potentials, however, there is an increased broadening of the action potentials in diabetic animals, so-called "spike broadening." The amplitude of the slow afterhyperpolarization elicited by a train of action potentials is indeed increased in diabetic animals. Interestingly, when the slow afterhyperpolarization is elicited by a Ca(2+) spike, there is no difference between control and diabetic rats. This indicates that the increased slow afterhyperpolarization in diabetes is likely to be due to an increased Ca(2+) influx resulting from the increased spike broadening. These data underscore the notion that the diabetic brain at the neuronal level shares properties with brain aging.

Action Potentials↗
PubMed2001

Laser in situ keratomileusis for treatment of residual myopia after photorefractive keratectomy.

PURPOSE: To evaluate the results of laser in situ keratomileusis after photorefractive keratectomy. METHODS: Eighty eyes of 80 patients with residual myopia after photorefractive keratectomy were reoperated with laser in situ keratomileusis. The study was retrospective. Laser in situ keratomileusis was performed using the automated corneal shaper microkeratome and Chiron Technolas 217-C d1 excimer laser. Data measured after laser in situ keratomileusis included uncorrected visual acuity, best-corrected visual acuity, refraction, haze, pachymetry, and keratometry. The follow-up was at least 12 +/- 1.6 months (range, 12 to 15 months). RESULTS: After laser in situ keratomileusis the mean spherical equivalent was -0.24 diopters +/- 0.78. (range, -3 to +1.5) at 12 months, and the mean uncorrected visual acuity was 0.76 diopters +/- 0.24 (range, 0.1 to 1). Sixty-five eyes (81.3%) had various degrees of haze after laser in situ keratomileusis. One eye (1.2%) lost 2 lines of best-corrected visual acuity. CONCLUSIONS: Laser in situ keratomileusis enhancement may be a good alternative to correct residual myopia and astigmatism after primary photorefractive keratectomy. Corneal haze is a common problem in these eyes, and the treatment after laser in situ keratomileusis enhancement should be the same as the treatment after primary photorefractive keratectomy.

Adolescent↗
PubMed2001

Laser in situ keratomileusis for undercorrection and overcorrection after radial keratotomy.

PURPOSE: To evaluate laser in situ keratomileusis (LASIK) in the management of overcorrection and undercorrection after radial keratotomy (RK). SETTING: Instituto Oftalmológico de Alicante, Alicante, Spain. METHODS: This prospective study comprised 20 eyes of 13 patients. The eyes were divided into 2 groups: 11 eyes that were overcorrected after RK and 9 eyes that were undercorrected after RK. Both groups were treated with LASIK after a mean interval of 22 months +/- 8.4 (SD) (range 12 to 45 months). The follow-up was 6 months. RESULTS: Six months after LASIK, there was a significant decrease in the spherical equivalent in both groups. Ninety-one percent of eyes in the overcorrected group and 89% in the undercorrected group were within +/-1.0 diopter of the intended correction. There was a significant improvement in the uncorrected visual acuity in both groups. In the overcorrected group, 91% showed no change or improvement in their best corrected visual acuity; in the undercorrected group, 89% showed no change or improvement. CONCLUSIONS: Laser in situ keratomileusis was relatively safe in treating both overcorrection and undercorrection after RK. With proper intraoperative precautions, LASIK can effectively treat the residual refractive defects after RK.

Adult↗
PubMed2001

Haze after laser in situ keratomileusis in eyes with previous photorefractive keratectomy.

In cases with previous photorefractive keratectomy (PRK), there is a risk of developing severe haze after laser in situ keratomileusis (LASIK). We report 3 patients (4 eyes) who developed severe haze after LASIK treatment for residual myopia following PRK. Both PRK and LASIK procedures were performed using the VISX 20/20 excimer laser. We report the grade of haze, amount of regression, and visual acuity after the patients were treated with topical steroids. In 2 eyes, the uncorrected visual acuity was 1.0 after 1 year with grade I haze. In the other 2 eyes, there was a residual refractive error, and the best spectacle-corrected visual acuity was 0.7 with grade II haze.

Administration, Topical↗
PubMed2000

Isotherm Model Analysis for the Adsorption of Cd (II), Cu (II), Ni (II), and Zn (II) on Anaerobically Digested Sludge.

Adsorption of Cd (II), Cu (II), Ni (II), and Zn (II) from aqueous solutions on anaerobically digested sludge has been investigated. Experimental data has been fit to Langmuir, Freundlich, and Redlich-Peterson isotherms to obtain the characteristic parameters of each model. Based on the maximum adsorption capacity obtained from the Langmuir and the Redlich-Peterson isotherm the affinity of the studied metals for the sludge has been established as Cu (II)>Cd (II)>Zn (II)>Ni (II). Adsorption tests from multimetal systems confirm the affinity order obtained in the individual metal tests. The adsorption capacity for Cu (II) measured in individual tests is not reduced by the presence of the other above referred metals. Desorption of Zn (II) and Cd (II) previously bound to the sludge in front of Cu (II) and HCl solutions is also reported. Copyright 2000 Academic Press.

Journal Article↗
PubMed2000

Synaptic activity modulates the induction of bidirectional synaptic changes in adult mouse hippocampus.

Activity-dependent synaptic plasticity is critical for learning and memory. Considerable attention has been paid to mechanisms that increase or decrease synaptic efficacy, referred to as long-term potentiation (LTP) and long-term depression (LTD), respectively. It is becoming apparent that synaptic activity also modulates the ability to elicit subsequent synaptic changes. We provide direct experimental evidence that this modulation is attributable, at least in part, to variations in the level of postsynaptic depolarization required for inducing plasticity. In slices from adult hippocampal CA1, a brief pairing protocol known to produce LTP can also induce LTD. The voltage-response function for the induction of LTD and LTP in naive synapses exhibits three parts: at a postsynaptic membrane potential during pairing (V(m)) </= -40 mV, no synaptic modification is obtained; at V(m) between -40 and -20 mV, LTD is induced; and, finally, at V(m) > -20 mV, LTP is generated. This function varies with initial synaptic efficacy. In depressed synapses, Theta(-), the V(m) above which LTD is generated, is shifted toward more depolarized V(ms) and Theta(+), the LTD-LTP crossover point or, equivalently, the V(m) above which LTP is induced, toward more polarized V(ms). Conversely in potentiated synapses, Theta(-) is shifted toward more polarized V(ms). Therefore synaptic activity changes synaptic efficacy and accordingly adjusts the voltages for eliciting subsequent synaptic modifications. The concomitant shifts in the voltages for inducing LTD and LTP in opposite directions promote synaptic potentiation and inhibit synaptic depression in depressed synapses and vice versa in potentiated synapses.

Animals↗
PubMed2000

Selective zonal ablations with excimer laser for correction of irregular astigmatism induced by refractive surgery.

OBJECTIVE: To demonstrate the safety and efficacy of selective zonal ablations with excimer laser for the correction of irregular astigmatism induced by refractive surgery. DESIGN: Thirty-one eyes of 26 patients. Retrospective and noncomparative case series. METHODS: The authors reviewed the medical results of the two series of consecutive cases of irregular astigmatism induced by refractive surgery. One group showed a topography map with a defined pattern: decentered ablation, decentered steep central island, central irregularity, and peripheral irregularity. The other group showed an irregular astigmatism without defined pattern. Selective zonal ablation was performed with a broad-beam excimer laser. Laser ablations were adjusted according to the Munnerlyn formula. A phototherapeutic keratectomy (PTK) mode was used in all cases without using viscous masking solution. MAIN OUTCOME MEASURES: Uncorrected visual acuity, best spectacle-corrected visual acuity (BCVA), and Holladay diagnostic summary (Eye Sys 2.000) were considered before and after surgery to obtain the qualitative and quantitative characteristics of the visual acuity and corneal topography. The corneal uniformity index (CUI) was used to evaluate the corneal surface changes induced by the selective zonal ablations in the correction of irregular astigmatism. It proved to be a useful tool in the understanding of astigmatic changes when it is not possible to apply the vector analysis. RESULTS: In group 1, the irregular astigmatism was significantly improved in 96.4% of cases. The CUI was improved from 58+/-0.3% to 87+/-0.8%, P < 0.005 (Student's t-test). The improvement of the BCVA was from 20/40+/-20/100 to 20/25+/-20/100, P < 0.005 (Student's t-test). The results obtained in group 2 were not clinically significant in terms of improvement of CUI and BCVA. CONCLUSIONS: Using the corneal topographic map as a guide, selective zonal ablations with excimer laser can be used to create a more regular corneal surface, when a defined pattern of topographic irregularities can be defined.

Adult↗
PubMed2000

Choroidal neovascularization in myopic eyes after photorefractive keratectomy.

PURPOSE: To evaluate the incidence, characteristics, and results of treatment of choroidal neovascularization (CNV) in myopic eyes corrected by photorefractive keratectomy (PRK). SETTING: University Miguel Hernández, Instituto Oftalmológico de Alicante, Alicante, Spain. METHODS: This study was of the incidence of CNV in 5936 consecutive eyes that had PRK for the correction of myopia. Mean follow-up was 38.5 months +/- 17.4 (SD). RESULTS: Extrafoveal CNV developed in the right eye of a 44-year-old woman 26 months after PRK for the correction of -12.00 diopters (D) of myopia. The follow-up after PRK was 38 months. Best corrected visual acuity (BCVA) before PRK was 20/40 (spherical equivalent [SE] -12.00 D). After PRK, BCVA was 20/32 SE -1.75 D). The CNV was treated by direct argon-green laser photocoagulation and did not recur in the subsequent 12 months). After CNV treatment, BCVA was 20/32 (SE -2.25 D). CONCLUSION: The incidence of CNV after PRK for myopia was low. Choroidal neovascularization is a possible complication in myopic eyes, and the risk exists before PRK. After PRK, the risk of CNV in myopic patients did not increase.

Adolescent↗
PubMed2000

Retinal detachment in myopic eyes after photorefractive keratectomy.

PURPOSE: To analyze the incidence and characteristics of retinal detachment (RD) in myopic patients who had photorefractive keratectomy (PRK). SETTING: Universidad Miguel Hernández, Instituto Oftalmológico de Alicante, Alicante, Spain. METHODS: The incidence of RD in 5936 consecutive eyes that had PRK to correct myopia was studied. Mean follow-up was 38.5 months +/- 17.4 (SD). RESULTS: Retinal detachment occurred in 5 eyes (0.08%); 2 in women and 3 in men. The mean interval between PRK and RD was 21. 00 +/- 15.89 months (range 9 to 48 months). The mean best corrected visual acuity (BCVA) after PRK and before RD development was 20/81 (range 20/200 to 20/25). After RD repair, the mean BCVA was 20/460 (range 20/2000 to 20/29). In 4 of the 5 eyes, BCVA after RD was within 1 line of the preoperative value; in 1 eye, it decreased from 20/40 to 20/2000. The mean spherical equivalent (SE) before RD treatment was -1.35 +/- 1.08 diopters (D) (range 0 to -3.00 D) and after RD treatment, -2.95 +/- 0.83 D (range -2.00 to -4.00 D). Differences between SE before and after RD treatment were statistically significant (P =.01, paired Student t test). CONCLUSIONS: The incidence of RD after PRK to correct myopia was 0.08%. In 4 of 5 eyes, there was little or no visual loss; but in the group as a whole, there was a significant increase in myopic SE.

Adolescent↗
PubMed1999

Induced endothelial cell loss in phacoemulsification using topical anesthesia plus intracameral lidocaine.

PURPOSE: To determine whether lidocaine 1% injected into the anterior chamber during phacoemulsification damaged the corneal endothelium. SETTING: Ophthalmology Service, University Hospital, San Juan de Alicante, Spain. METHODS: In 19 eyes of 19 patients, small incision phacoemulsification and foldable lens (AcrySof MA60BM) implantation was performed using topical anesthesia supplemented by lidocaine 1% (0.3 mL) in the anterior chamber. All cases were operated by the same surgeon. Specular microscopy was performed before surgery and 1 and 3 months postoperatively. RESULTS: Mean endothelial cell loss was 5.41% +/- 8.50 (SD) at 1 month and 5.93 +/- 8.50% at 3 months. CONCLUSIONS: The endothelial damage was similar to that described by other authors, suggesting that phacoemulsification surgery can be performed successfully using topical anesthesia supplemented by lidocaine 1% in the anterior chamber.

Aged↗
PubMed1999

Photorefractive keratectomy for residual myopia after cataract surgery.

PURPOSE: To evaluate the effectiveness, predictability, and safety of photorefractive keratectomy (PRK) for correcting residual myopia and myopic astigmatism after cataract surgery with intraocular lens implantation. SETTING: Refractive Surgery and Cornea Unit, Instituto Oftalmológico de Alicante, Alicante, Spain. METHODS: Thirty consecutive eyes (30 patients) had PRK for residual myopia after cataract surgery. Surface PRK with a VISX Twenty-Twenty excimer laser was used in all patients. Follow-up was 1 year. RESULTS: Before PRK, no eye had an uncorrected visual acuity (UCVA) of 20/40 or better. Twelve months after PRK, 16 eyes (53.33%) had a UCVA of 20/40 or better. After PRK, best corrected visual acuity (BCVA) improved 1 line or more in 14 eyes (46.66%) over the preoperative values, and 15 eyes (50.00%) had the same BCVA as before PRK. Mean pre-PRK refraction of -5.00 diopters (D) +/- 2.50 (SD) decreased significantly to -0.25 +/- 0.50 D at 12 months (P < .001). At 12 months, the spherical equivalent was within +/- 1.00 D of emmetropia in 27 eyes (90.00%). No vision-threatening complications occurred. CONCLUSION: Photorefractive keratectomy was an effective, predictable, and safe procedure for correcting residual myopia and myopic astigmatism after cataract surgery.

Aged↗
PubMed1998

Metallic fragment deposits during phacoemulsification.

PURPOSE: To describe the nature and origin of foreign metallic intraocular bodies appearing after phacoemulsification. SETTING: Department of Ophthalmology, University Hospital of San Juan, Alicante, Spain. METHODS: Two metallic particles, 1 extracted during surgery and the other from a patient in whom surgery had been performed, were studied by scanning electronic microscopy and X-ray dispersive energy spectroscopy. Also evaluated were all metallic elements used in phacoemulsification including phaco tips, Sinskey hooks, and handpieces. A morphologic analysis at various magnifications was performed and the composition of the elements studied. RESULTS: Scanning electronic microscopy showed irregularities of the phaco tip and Sinskey hook after their use. Spectrographic analysis found that the phaco tip was mainly aluminum and titanium; the Sinskey hook, iron, chromium, cobalt, and nickel; the handpiece, iron, chromium, and nickel; and the 2 metallic particles, iron, chromium, and nickel, the same as the handpiece. CONCLUSION: The metallic particles extracted corresponded to those of the phaco handpiece. Vibration during prolonged use of the phacoemulsifier probably caused these particles to chip off the handpiece.

Alloys↗