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A Böcking

Publications and source records attributed to A Böcking.

At least 91 records · Page 5Linked to original sources
PubMed1989

Bowenoid papulosis. Classification as a low-grade in situ carcinoma of the epidermis on the basis of histomorphologic and DNA ploidy studies.

The nature of bowenoid papulosis was investigated by a comparative investigation of 12 biopsy specimens of this lesion, 19 biopsy specimens of Bowen's disease, 14 biopsy specimens of squamous cell carcinoma and 10 biopsy specimens of seborrheic keratosis. In addition to conventional histomorphologic and cytomorphologic studies, nuclear DNA measurements on single cells isolated from tissue blocks were performed using a TV image analysis system combined with an automatic microscope. Two parameters, the "5c exceeding rate" (5cER) and the "2c deviation index" (2cDI), were computed from the single-cell DNA values to arrive at a "DNA diagnosis" and a "DNA malignancy grade" (DNA-MG). All specimens of bowenoid papulosis and Bowen's disease were morphologically diagnosed as in situ carcinomas of the epidermis; a DNA diagnosis of malignant was rendered in all of these specimens due to the detection of aneuploid nuclei (5cER greater than or equal to 1). DNA diagnoses of malignant were also rendered on all specimens of squamous cell carcinoma (100% sensitivity) while DNA diagnoses of benign were rendered in all specimens of seborrheic keratosis (100% specificity). The mean DNA-MG for bowenoid papulosis (0.69) was significantly lower than that for Bowen's disease (1.04) and squamous cell carcinoma (1.15). The mean morphologic (Broder's) grade of malignancy was also lower for bowenoid papulosis than for Bowen's disease and squamous cell carcinoma. HPV 16 DNA was detected in 10 of 12 specimens of bowenoid papulosis. Thus, the results of DNA image cytometry and morphologic investigation suggest that bowenoid papulosis is a low-grade carcinoma in situ of the epidermis.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1989

Representativity and reproducibility of DNA malignancy grading in different carcinomas.

The reproducibility of the determination of the "DNA malignancy grade" (DNA-MG) was tested in 56 carcinomas of the colon, breast and lung while its representativity was tested on 195 slides from 65 tumors of the colon, breast and lung. DNA measurements were performed on Feulgen-stained smears with the TAS Plus TV-based image analysis system combined with an automated microscope. The variance of the DNA values of tumor cells around the 2c peak, the "2c deviation index" (2cDI), was taken as a basis for the computation of the DNA-MG, which ranges on a continuous scale from 0.01 to 3.00. The representativity, analyzed by comparison of the DNA-MGs measured in three different areas of the same tumor greater than or equal to 1.5 cm apart from each other, yielded an 81% agreement. No significant differences between DNA-MGs of these areas were found. The intraobserver and interobserver reproducibilities of the DNA grading system, investigated by repeated DNA measurements, were 83.9% and 82.2%, respectively. In comparison, histopathologic grading of the 27 breast cancers studied yielded 65% intraobserver and 57% interobserver reproducibilities and 66% representativity.

Breast Neoplasms↗
PubMed1989

Diagnostic and prognostic value of DNA cytometry in gynecologic cytology.

A survey of the diagnostic and prognostic value of DNA cytometric measurements in gynecologic tumors is given. In slight-to-moderate epithelial dysplasias of the uterine cervix, morphologic studies alone cannot make a definite distinction between benignity and malignancy, nor can they identify all precancerous lesions. DNA cytometry may help in these cases to detect prospective malignancy. Cytologic and histologic grading of malignancy often does not provide correct information about the prospective behavior of an individual tumor; its reliability is hampered by low interobserver reproducibilities. DNA cytometry may supplement subjective morphologic grading by providing objective and reproducible prognostic indices. The advantages and disadvantages of TV-based image cytometry and flow cytometry for application in routine gynecologic pathology and the different attempts at diagnostic DNA interpretation are discussed.

Breast Neoplasms↗
PubMed1989

DNA grading of malignancy in breast cancer. Prognostic validity, reproducibility and comparison with other classifications.

The prognostic significance of the "DNA malignancy grade" (DNA-MG) was tested in a series of 104 breast cancer patients in comparison with TNM staging, histomorphologic grading according to Bloom and Richardson, mean nuclear area (MNA) and DNA-histogram classification according to Auer. The reproducibility and representativity of the grading systems were investigated, and their results in primary tumors and lymph node metastases were compared. The scalar DNA-MG was assessed on monolayer smears prepared from paraffin-embedded tissues; the smears were automatically Feulgen stained and used for rapid interactive DNA cytometric evaluation by an automated microscope and a TV image-analysis system. TNM staging showed the highest correlation with survival, followed by histomorphologic grading and DNA-MG; MNA and the DNA-histogram classification failed to give statistically significant prognostic information. Both histomorphologic grading and DNA-MG were identified as parameters adding independent prognostic information to the TNM staging. However, only DNA-MG demonstrated an acceptable reliability, with small 95% ranges between repeated measurements within the primary tumor (+/- 0.3 DNA-MG) and a strong correlation between the results in the primary tumor and its lymph node metastases. These findings show that the DNA-MG is a valid and reliable prognostic index that adds significant prognostic information to TNM staging.

Aged↗
PubMed1989

Diagnosis of prospective malignancy in koilocytic dysplasias of the cervix with DNA cytometry.

DNA cytometric measurements were performed with a TV image analysis system on 90 routine cytologic cervical smears from 18 women after automatic restaining of the smears with the Feulgen method. In the cytologic follow-up all the patients revealed borderline lesions (mild to moderate dysplasia) of the epithelium with koilocytosis. Since DNA aneuploidy is widely accepted as a marker for malignancy, an attempt was made to obtain a DNA diagnosis of prospective malignancy in these cases on the basis of the detection of single aneuploid cells. Because koilocytosis strongly suggests human papillomavirus infection, euploid polyploidy might result in up to 8c (c = haploid amount of DNA). Therefore, the aneuploidy detection threshold was set to 9c, excluding values around 16c and 32c (+/- 12.5%) to avoid false-positive diagnoses in cases of higher polyploidy values. The DNA diagnosis of malignancy was made in 13 patients. In 12 of them, carcinoma in situ was diagnosed in the histologic follow-up, resulting in 100% sensitivity of the DNA diagnosis. In five patients no cells greater than 9c were measured, leading to a benign DNA diagnosis. In the histologic follow-up, six patients revealed normal epithelium or moderate dysplasia. Thus, the specificity of the DNA diagnosis was 83.3%.

Adult↗
PubMed1988

Cellular invasion on the surface of intraocular lenses. In vivo cytological observations following lens implantation.

Cellular growth on intraocular lenses can be observed by using a specular microscope. We examined in vivo the cellular pattern on the surface of implanted lenses during the early postoperative period in 63 patients. In every case inflammatory cells were noted on the lens surface at some point during the observation period. These often showed a spindle-like appearance resembling fibroblasts. The density of the cell population increased during the following days but usually did not exceed 100 cells/mm2. From the end of the first week, multinucleated giant cells may appear. In one case, a patient with a severe postoperative iritis and hypopyon demonstrated an extremely pronounced and long-lasting cellular proliferation.

Cataract Extraction↗
PubMed1988

Diagnostic and prognostic value of DNA image cytometry in myelodysplasia.

The DNA content of erythropoietic cells from 10 patients with refractory anaemia (RA) with megaloblastic changes, who subsequently developed acute non-lymphoblastic leukaemia (ANL), and from seven patients with megaloblastic marrow aspirates due to pernicious anaemia were compared by DNA image cytometry. The DNA distribution, the rate of aneuploid cells exceeding 5c (5cER), and the square deviation index of DNA values from the normal 2c-peak (2cDI) were recorded. Both variables were of diagnostic and prognostic importance for epithelial tumours, malignant lymphomas, and dysplastic lesions. A rate of 5cER greater than 0 was found in eight of 10 myelodysplastic, but in none of seven control cases. Hypodiploidy was equally pronounced in both groups of patients. The 5cE had the highest discriminative value of all variables calculated. The 2cDI was not significantly different in either group. In pernicious anaemia the 2cDI depended mainly on the percentage of S cells, reflecting the defect of DNA synthesis. In RA with megaloblastosis the 2cDI correlated with the percentage of G2 cells, reflecting G2 arrest. In the myelodysplastic group the 2cDI correlated positively with the length of time until ANL developed, indicating the prognostic relevance of 2cDI. Our findings show that in megaloblastic anaemia DNA image cytometry can distinguish myelodysplasia from pernicious anaemia and that it also provides prognostic information.

Acute Disease↗
PubMed1987

Cytological grading of therapy-induced tumor regression in prostatic carcinoma: proposal of a new system.

Proposed in this article is a new system for the cytological grading of therapy-induced regression of prostatic carcinoma. Reversible and irreversible criteria in cytoplasm, nuclei, and nucleoli are distinguished and rated independently of each other on a scale of 0 to 2; the cellular regression grades r1-r3 are the sum of rating numbers. Criteria that are invalid for grading regression are pointed out. Results of the clinical testing of this grading system are presented.

Biopsy, Needle↗
PubMed1987

[Biomicroscopy of an intraocular lens. Comparison of specular microscopy findings with the cytologic specimen].

Seventeen months after implanting an anterior chamber intraocular lens the lens had to be removed because of secondary glaucoma and retinal hemorrhage. The lens had been examined 5 days prior to surgery. It appeared that histiocytic giant cells and fibroblasts had settled on its surface. This was subsequently confirmed by cytological studies. It therefore appears possible to describe an in vivo differential cytology and to diagnose the type of inflammatory reaction in vivo.

Aged↗
PubMed1986

[AIDS in a woman having had sexual relations with a patient with hemophilia A. Characteristic findings in DNA image cytometry].

A 37-year-old female patient reported marked weight loss, prolonged alopecia, recurrent infections and watery diarrhoea. Examination revealed Salmonella infection, candidiasis and immunological signs of previous toxoplasmosis. Between 1978 and 1981, the patient had had close sexual relations to a patient with haemophilia A. Due to this fact, AIDS was suspected. Serological tests for HIV were not available at the time. The findings in DNA image cytometry (nuclear DNA inclusion bodies, polyploid lymphocyte nuclei and binuclear lymphocytes) suggested a viral infection of the lymphoid cells. Electron microscopy revealed in hepatocytes and cerebral cells intranuclear inclusion bodies whose size and contents were not compatible with an infection caused by cytomegalovirus, herpes virus or Epstein-Barr virus. In autopsy, infections of various organ systems such as pneumonia, tracheobronchitis, urocystitis, pyelonephritis, Candida oesophagitis and enteritis were found.

Acquired Immunodeficiency Syndrome↗
PubMed1986

DNA image cytometry in acquired immune deficiency syndrome (AIDS).

In nine cases with the acquired immune deficiency syndrome (AIDS), including four stage I cases, three stage II cases and two stage III cases, DNA image cytometry was performed on Feulgen-stained lymph node imprint smears. Diploidy was found in three cases, tetraploidy in three cases and octoploidy in two cases. Aneuploid DNA distribution patterns were not seen. The lymphoid cells showed an enormously increased proliferation rate. Two cases in stage I revealed characteristic intranuclear DNA inclusions in lymphoid cells. These results indicate that DNA image cytometry may be useful as an adjunct to surgical pathology in certain cases to assist in the differential diagnosis between AIDS and benign conditions of the lymphoid system as well as between AIDS and malignant lymphomas, which usually have aneuploid DNA patterns.

Acquired Immunodeficiency Syndrome↗
PubMed1986

[Precancerous stages of the oral mucosa--possible identification of obligatory precancerous conditions].

Macroscopically and microscopically precancerous lesions of the oral mucosa cannot be diagnosed with sufficient accuracy. Dysplasias represent a particular diagnostic difficulty. The reproducibility of grading of dysplasias may be increased by the quantification of morphological parameters using image analysis systems. However an unequivocal identification of obligatory precancerous lesions cannot be attained by this method. Measurement of the nuclear DNA content represents a non-morphological method for the identification of obligatory precancerous lesions. The diagnostic criterion of malignancy is aneuploidy. Structural or numerical chromosomal aberrations that are absent in benign tumours and normal cells typify aneuploidy. Aneuploidy is manifested by an atypical nuclear DNA distribution that can be identified objectively by suitable algorithms. Dysplasias of the squamous epithelium with aneuploid nuclear DNA contents are regarded as obligatory precancerous lesions. DNA measurements, computation of data and printout of a 'DNA diagnosis' are possible with a TV-image analysis system and an automated microscope within 30 min.

Carcinoma in Situ↗
PubMed1985

Diagnosis and grading of malignancy in squamous epithelial lesions of the larynx with DNA cytophotometry.

For diagnostic purposes 14 histologically benign, 15 dysplastic, and 16 malignant squamous epithelial lesions were subjected to DNA cytophotometry. Results were computed according to an algorithm for DNA diagnosis and grading of malignancy. In cases of unequivocally malignant or benign lesions all DNA diagnoses agreed with the histologic diagnoses. In one case the allegedly faulty DNA diagnosis of cancer was proven to be correct on clinical follow-up. Within the group of histologically mild to moderate dysplasias the algorithm identified four cases as malignant that were proved to be malignant either in the follow-up or at another site of the same lesion. With the aid of the DNA malignancy grade two groups of patients with squamous epithelial carcinomas of the larynx could be discerned with a highly significant difference according to their survival times.

Adult↗
PubMed1985

Early detection of precancerous lesions in dysplasias of the lung by rapid DNA image cytometry.

Hematoxylin-and-eosin-stained cytologic smears of sputum from 28 patients with dysplastic and suspicious cell findings were subjected to DNA image cytometry after Feulgen restaining. The nuclear DNA contents were measured with a TV-based image-analysis system, the Leitz TAS plus, combined with an automatic microscope. Computation of DNA data was performed according to an algorithm for the diagnosis and grading of malignancy. Of the 19 cases that were proven to be malignant in the follow-up, either by histologic examination, sputum cytology, fine needle aspiration biopsy or autopsy, the algorithm identified 17 as malignant in a stage (dysplasia) in which cytology was not yet able to present a definite diagnosis of malignancy. Only two cases of bronchial carcinoma were not detected in the state of dysplasia by this procedure. The periods between the DNA diagnosis of malignancy in dysplasia and the morphologic evidence of cancer varied from three days up to six months. Of the 11 cases that had been classified as benign by the algorithm, 9 were confirmed as benign during the clinical follow-up. Rapid DNA image cytometry appears able to separate squamous dysplasias of the lung into precancerous and nonprecancerous lesions.

Adenocarcinoma↗
PubMed1985

DNA grading of malignancy and tumor regression in prostatic carcinoma under hormone therapy.

The prognostic value of the DNA malignancy grade (DNA-MG) and of the DNA regression index (DNA-RI) for survival was tested in 19 patients with prostatic carcinoma under hormone therapy. Measurements were performed on repeated Feulgen-stained transrectal aspiration biopsy smears by DNA image cytometry. The DNA-MG was demonstrated to be an objective scalar index of high prognostic value for the individual patient. The prospective tumor response to hormone therapy could be predicted from the DNA-MG. Independently of the DNA-MG, the DNA-RI provided additional prognostic information concerning tumor regression and survival of prostatic cancer patients under conservative therapy. The determination of DNA-RI allows the early identification of an ineffective conservative therapy.

Aged↗