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Biomedical subjects

A Böcking

Publications and source records attributed to A Böcking.

At least 127 records · Page 7Linked to original sources
PubMed1976

Esterase. XXIII. Electron microscopical demonstration of non-specific esterases in the jejunum of the mouse (Mus musc.) with two quinoline derivatives.

The intracellular distribution of non specific esterases in various villous cells of mouse jejunum was investigated using two substrates, 8-acetoxiquinoline (Q-O-2) and 8-acetyl mercaptoquinoline (Q-S-2) respectively. With the more selectively staining Q-S-2 a uniform reaction was demonstrated in all enterocytes which was mainly located in the endoplasmic reticulum and the nuclear envelope. Possibly it is a matter of a single enzyme being hardly detachable from the membranes. With the non selectively staining Q-O-2 several esterases were demonstrated Es-2, adn Es-9 among them. The main reaction was found in columnar and Goblet cells, in both at all cellular membranes and in the mitochondrial matrix, additionally in cisterns and vesicles of the endoplasmic reticulum and of the Golgi apparatus and at lipid droplets of the former, finally at mucous droplets of the latter. An extracellular reaction was found in the intercellular cleft and between the microvilli of the brush border.

Animals↗
PubMed1976

Esterase. XXII. Cellular and subcellular localisation of the Es-9 esterase in mouse kidney.

Among the various esterases of the mouse kidney the Es-9 esterase is outstanding in that it is fully repressed in the cortical proximal tubulus in the absence of testosterone but is constitutive in the terminal parts of the proximal tubulus. Thus, this enzyme is regulated in a different way in closely neighboured sections of the tubular system. Comparing the disc electrophoretic patterns of the kidney esterases with the distribution of esterases in ultrathin sections as revealed by electron microscopy it is concluded that the Es-9 esterase is located in the mitochondria.

Animals↗
PubMed1975

Esterase XIX. Biochemical and ultrahistochemical investigations of the non specific esterase in nuclei from mouse liver.

The present investigations reveal that the non specific esterase from mouse liver nuclei is exclusively located in the perinuclear cistern and that the nuclear chromatin does not contain any esterase as a rule. However, esterase is associated with lipid droplets which are seldomly found within nuclei. Two different esterases are demonstrated with the employed substrates in the nuclear envelope. It is exemplified that the esterase, found in isolated nuclei, can neither be understood as qualitative nor as quantitative equivalent of the esterase demonstrated ultrahistochemically at nuclear membranes.

Animals↗
PubMedDate not supplied

Cytological vs histological evaluation of percutaneous biopsies.

Fine-needle aspiration cytology yields sufficient diagnostic accuracy to compete with histology from punch biopsy for cancers of the lung and the prostate. For tumors of the breast, pancreas, thyroid, salivary glands, and kidneys, fine-needle aspiration cytology yields sufficient sensitivity and specificity when performed preoperatively; the exact tumor typing will follow surgical excision. In palpable lymph nodes, adrenals, and lungs, fine-needle aspiration biopsy is sufficient if metastases from a known primary tumor are suspected. Indications for punch biopsies and histological investigation are tumors of the liver, posterior and anterior mediastinum, retroperitoneum, soft tissues, and bone. In these conditions, cytological investigation alone provides insufficient typing accuracy. Suspected lymphomas in a retroperitoneal or mediastinal location should be punctured and may be classified from punch biopsies if the node is not easily reached by surgery. There are no indications for percutaneous biopsies in tumors of the skin, testes, and ovaries. Percutaneous fine-needle aspiration biopsies in general are associated with significantly lower complication rates than punch biopsies.

Biopsy, Needle↗
PubMedDate not supplied

CT-guided percutaneous large-bore biopsies in benign and malignant pediatric lesions.

Thirty-nine consecutive pediatric patients underwent large-bore biopsies of 41 lesions under computed tomography (CT) guidance; general anesthesia was not required. Adequate material was obtained in all patients. There were no false-negative or false-positive results. Typing accuracy was 100% in malignant disease and 92% in benign lesions. No significant complications occurred. Percutaneous large-bore biopsy is a safe, reliable, and time-saving method that obviates the need for open surgery and general anesthesia in the diagnosis of primarily non-resectable lesions, or lesions that do not require resection. Another major advantage over open surgery in malignant disease is the absence of delay in starting urgently needed chemotherapy.

Adolescent↗
PubMedDate not supplied

Single pass aspiration biopsy for repeat sampling--evaluation in 726 cases: technical note.

A simple technique is described for performing repeat samplings in fine-needle aspirations (FNA) without the need for further needle insertions. This technique is advocated for puncture of deeply situated masses and has proved useful in FNA of 726 lesions in various regions yielding a low rate of inadequate material (7.3%), false-negative results (2.5%), and complications. When compared with standard techniques, this technique has the essential advantage that the aspirated material can be judged visually for adequacy before the biopsy needle is withdrawn.

Biopsy, Needle↗
PubMedDate not supplied

["Primary" pulmonary hypertension and systemic lupus erythematosus in adolescence].

Report is given on a 15 years old girl suffering from severe "primary" pulmonary hypertension (PPH) of the plexogenic type without underlying pulmonary parenchymal or cardiac disease. Diagnosis was established by cardiac catheterization and lung biopsy. At the same time she had clinical symptoms and laboratory signs of systemic lupus erythematosus (SLE). Looking back there had been for some years symptoms suggestive of both diseases. PPH is characterized by several histopathological criteria whereas its aetiology is unknown. A causal connexion between PPH and connective tissue diseases, especially SLE, is probable because of their repeatedly observed coincidence. Raynauds phenomenon, sometimes included in some connective tissue syndrome, and vascular alterations in PPH could be explained by sustained vasoconstrictive mechanisms caused by hyperractive pulmonary vessels. Another possible pathogenetic role relates to immune complexes causing vascular changes. It is assumed that, besides the classical pulmonary involvement, vascular changes of "primary" pulmonary hypertension themselves may represent a typical albeit rare pulmonary manifestation of SLE or other connective tissue disease.

Adolescent↗
PubMedDate not supplied

Summary statement on quantitative cytochemistry (DNA and molecular biology): Task Force 8.

OBJECTIVE: To reach consensus on the application of quantitative cytochemical analysis of chromosomal and DNA aneuploidy in cervical cytopathology. CONCLUSION: The current Pap test has limited specificity to predict cancer and its truly progressive pre-malignant lesions. Infection with human papillomavirus may trigger genetic instability, hyperproliferation and immortalization of the cervical mucosa and cause cervical cancer. Several related molecular markers have been shown to be informative about this neoplastic process. Quantitative analysis of chromosomal and DNA aneuploidy has been shown to be an important tool for identifying (progression to) high grade squamous intraepithelial lesions. A high degree of standardization (material handling, calibration and quality control, measurement and interpretation of results) is required for accurate and reproducible measurements. Areas for further study are presented.

Aneuploidy↗
PubMedDate not supplied

Semiautomated monolayer preparation of bronchial secretions using AutoCyte PREP.

OBJECTIVE: Development of a method for semiautomated preparation of purified, representative and conventionally stained monolayer smears from bronchial secretions suitable for subjective and/or automated cytodiagnosis. STUDY DESIGN: Bronchial secretions from 50 patients with and 48 without carcinoma cells of different types were collected in Saccomanno's fixative. After routine pick-and-smear processing, residual material was subjected to a mucolytic agent (ammonium thioglycolate). Separation of cells was performed by differential centrifugation through aqueous sucrose. The pellet was automatically processed by the AutoCyte PREP system. RESULTS: Slides revealed well-preserved, slightly shrunken, homogeneously distributed cells devoid of mucus, cellular debris and bacteria in monolayer arrangement nearly without overlap. Granulocytes were eliminated to a large extent. Comparison with pick-and-smear specimens showed more tumor cells per square centimeter of slide surface in 100% of AutoCyte PREP slides. The number of tumor cells per AutoCyte PREP slide was higher in 46% and lower in 54%. Selecting slides at random and requiring at least 10 abnormal cells to establish a tumor diagnosis were achieved in 82.7% if only one, in 88.0% if two and 94.0% if seven or eight AutoCyte PREP slides were investigated. CONCLUSION: The semiautomated method yielded conventionally stained, purified monolayer smears from bronchial secretions with cellular morphology suitable for evaluation by cytologists and screening machines. Representativity of AutoCyte PREP monolayers was superior to that of pick-and-smear slides.

Adenocarcinoma↗
PubMedDate not supplied

Histological effects of local microwave hyperthermia in prostatic cancer.

Recent investigations have shown that hyperthermia can reduce the volume of the prostate and improve local symptoms in patients with carcinoma of the prostate. Histological examinations of the effect of hyperthermia on prostatic cancer tissue, however, have not been performed systematically until now. Thus, we initiated a study to investigate the effects of heat on prostatic cancer as a prerequisite for further clinical trials on hyperthermia as treatment for prostatic cancer. Twenty patients with untreated prostatic cancer underwent local hyperthermia (915 MHz), each receiving four sessions of 60 min each. The intraprostatic temperature was 42-43 degrees C. Histological specimens of the prostate were taken before the treatment and 1-2 weeks after the last hyperthermia session. Hyperthermia produced hyperaemic alterations of the prostatic stroma and a diffuse oedema with interstitial lymphoplasmacellular infiltration. Definite signs of tumour cell necrosis, however, could not be seen in any of the patients. Hence the shrinkage of prostatic tumours described earlier cannot be explained by histologically proven tumour cell destruction. Thus hyperthermia is not adequate as a single treatment for prostatic cancer. Hyperthermia may, however, be useful as part of integrated therapy regimens together with cytostatic or hormonal agents and radiotherapy because of hyperaemic, chemo- and radiosensitizing effects.

Adenocarcinoma↗
PubMedDate not supplied

Carcinoma of the extrahepatic biliary system: correlation of clinical, pathological, histological and DNA-cytometric parameters with prognosis.

BACKGROUND: Studies concerning the prognosis of all carcinomas of the extrahepatic biliary system together (EBC) do not exist, so far. A detailed and valid histopathological grading-system is lacking and the prognostic relevance of DNA-cytometric parameters is not clear. METHODS: 81 patients with EBC underwent surgical treatment. Clinical, pathological, histomorphological and DNA-cytometric parameters were evaluated. The influence on prognosis was tested in univariate and multivariate analysis. RESULTS: Apart from significant perioperative mortality (17%) survival was markedly dependent on the presence of tumor remnants in resection margins. After incomplete and complete resection, the median survival was 5 and 19 months, respectively (p < 0.0001). Further parameters with significant prognostic relevance were tumor localization, pTNM-stage, period of complaints, presence of gallstones, histological type and DNA-index. Multivariate analysis however demonstrated only two independent prognostic factors: status of resection margins and tumor localization. CONCLUSIONS: Histomorphological- and DNA-cytometric parameters failed to demonstrate an additional significant influence on the prognosis in EBCs. Apart from perioperative mortality, the survival of patients with EBC predominantly depends on tumor localization and the status of resection margins.

Adenocarcinoma↗
PubMedDate not supplied

Diagnosis of bronchial carcinoma on sections of paraffin-embedded sputum. Sensitivity and specificity of an alternative to routine cytology.

The diagnostic accuracy of sputum cytology for the diagnosis of bronchial carcinoma using paraffin-embedded, serially sectioned and hematoxylin and eosin-stained specimens was tested in 4,297 sputum samples from 1,889 patients, 219 of whom had bronchial carcinoma. The diagnostic sensitivity depended mainly on the number of investigated samples and was 85.4% with three sufficient sputa. The sensitivity was not influenced by the histologic types, location or TNM stage of the tumor. The specificity of the method was 99.5%. In three cases localization of sputum cytologically diagnosed bronchial carcinomas was not possible immediately (occult carcinomas, pTx); in two of these cases the bronchial carcinomas were located during follow-up. The third patient died without verification of the cytologic diagnosis. According to our results, sputum cytology on serial sections is a valuable instrument for mass screening of high-risk groups for the early detection of bronchial carcinoma. Lower sensitivities of sputum cytology in mass screening programs for the early diagnosis of lung cancer are discussed critically.

Carcinoma, Bronchogenic↗