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A Bakunowicz-Lazarczyk

Publications and source records attributed to A Bakunowicz-Lazarczyk.

At least 19 recordsLinked to original sources
PubMed2006

Flow cytometric analysis of HLA-DR antigen in conjunctival epithelial cells of patients with cystic fibrosis.

PURPOSE: Cystic fibrosis (CF) is an autosomal-recessive genetic disorder. The disease affects all secretory epithelia including the eye and belongs to the group of ocular surface epithelial diseases, termed keratoconjunctivitis sicca that develop in dry eye. In the pathogenesis of dry eye, inflammation plays a crucial role. The aim of this study was to investigate the expression of HLA-DR on conjunctival epithelial cells from patients with CF. MATERIALS AND METHODS: Twenty-five patients with CF and 25 normal subjects underwent ocular examination. Tear film break-up time (TBUT), Schirmer test, lissamine green staining, and conjunctival impression cytology were carried out. Cells were processed for flow cytometry, by using monoclonal antibodies to HLA-DR. RESULTS: The Schirmer test and TBUT scores were significantly lower in CF patients compared with controls. A significant increase of HLA-DR expression on epithelial cells was found in patients with CF compared with normal eyes. The Schirmer and TBUT test were positively correlated with HLA-DR expression for the percentage of cells. CONCLUSION: These results suggest that conjunctival epithelial cells play an important proinflammatory role in ocular changes in CF patients. Our findings confirm the presence of an inflammatory background and the immune nature of this disease. HLA-DR measurement might be a useful method for monitoring of inflammatory processes in the conjunctiva and could be helpful in the use of anti-inflammatory drugs in the treatment of ocular findings in CF patients.

Adolescent↗
PubMed2001

Expression of P53 protein in primary uveal melanoma.

Malignant melanomas are the commonest intraocular tumours. The aim of the present study was the immunohistochemical analysis of P53 protein expression in 39 primary ocular melanomas treated surgically in the years 1983-1997. Expression of P53 was estimated semiquantitatively with the use of a light microscope at a magnification of x 400. At least 200 neoplastic cells per sample were analysed. The expression of P53 in 3% of melanoma cells was considered the threshold of positive reaction. The results were subjected to statistical analysis with exact Fischer's test. No statistically significant correlations were found between P53 expression and anatomoclinical properties of tumours, although increased P53 expression was observed in epithelioid-cellular melanomas and in tumours growing in women. A lack of statistically significant relationship between P53 protein level and the parameters examined, and morphological specificity of ocular melanomas hindering immunohistochemical analysis, indicate the necessity for studies on a wider group of tumours or/and use of molecular biology techniques to establish possible relations between P53 gene mutation and ocular melanoma biology.

Adult↗
PubMed2001

Cellular immunity in pediatric uveitis.

To evaluate cellular immunity in children with uveitis, we used the flow cytometry method to study the T lymphocyte subpopulation in peripheral blood. Cytometric analysis of peripheral blood lymphocytes using fluorochrome-marked monoclonal antibodies was carried out in 29 children aged 6-18 years with parasitic uveitis (group A) and with uveitis of another etiology (group B). The control group consisted of 28 healthy children aged 6-15 years. A substantially higher percentage of B lymphocytes (19.0 +/- 6.03) was observed in the children with parasitic uveitis than was observed in the healthy children. Moreover, the absolute values were higher in groups A (1.60 +/- 0.53) and B (1.81 +/- 0.40) than in controls (1.50 +/- 0.38). The lowest percentage of CD4+ lymphocytes (34.25 +/- 6.06) and the highest percentage of CD8+ lymphocytes (31.14 +/- 6.50) was found in children with parasitic uveitis. The lowest index CD4+/CD8+ (1.29 +/- 0.53) in the group of children with parasitic uveitis reflected severe disorders in response regulation. However, the percentage values of natural killer cells was lower in the two groups of patients than in controls. These results suggest that quantitative changes in the subpopulation of peripheral blood lymphocytes are more pronounced in parasitic uveitis.

Adolescent↗
PubMed2000

[A case of 9-year old child with craniopharyngioma].

Craniopharyngiomas are slow-growing tumours arising from vestigal remnants of Rathke's pouch along the pituitary stalk. A case of 9-year old child with craniopharyngioma is presented. Immediate diagnostic and surgical treatment improved clinical condition.

Child↗
PubMed1999

Comparative studies of morphological changes and interleukin concentration in subretinal fluid of patients with retinal detachment.

Morphological evaluation was performed with the light and scanning electron microscope of subretinal fluids (SRF) collected during operations from 36 patients with rhegmatogenous retinal detachment. Duration of the disease was 3 weeks, 5-8 weeks, 10 weeks and 6 months. The immunoenzymatic method was used to establish the presence of interleukins IL-1beta, IL-2, IL-4 and IL-6 in SRF. It was revealed that the differentiation of the morphological picture of SRF depended on the duration of detachment. Most cell-rich SRF came from the 3-week detachment. Mononuclear cells were predominant in that period (macrophages, lymphocytes). In the later period (over 8 weeks) a decrease in the number of cells and a change in cellular composition were noted in the fluids examined, while after six months of detachment normal cellular elements were absent. In all time intervals analyzed, no significant increase in IL-1beta, IL-2 and IL-4 was noted in SRF. However, a significant quantity of IL-6 was found, particularly in the 5- to 8-week detachment. The authors assume that the presence of a large number of inflow cells in 'fresh' detachment can trigger a variety of inflammation-based immunological processes which stimulate the development of vitreoretinal proliferation.

Adult↗
PubMed1999

[Sub-retinal fluid interleukin-10 (Il-10) and interleukin-13 (Il-13) concentration in patients with retinal detachment].

PURPOSE: To evaluate the presence of IL-10 and IL-13 in subretinal fluid of patients with rhegmatogenous retinal detachment. MATERIAL AND METHOD: The studies comprised 14 patients on with retinal detachment operated. The presence of cytokines was evaluated using immunoenzymatic assay. RESULTS: IL-10 and IL-13 were found in all subretinal fluid samples. Our results suggest that the presence of IL-10 and IL-13 has the influence on suppression of inflammatory agent release and activity during retinal detachment.

Adolescent↗
PubMed1999

[The evaluation of contrast sensitivity in children and adolescents with insulin-dependent diabetes mellitus].

PURPOSE: The evaluation of contrast sensitivity in children and adolescents with type I diabetes mellitus with and without retinopathy, taking into account metabolic control. MATERIAL AND METHODS: We examined 100 young patients (71 without retinopathy and 29 with background retinopathy on fluorescein angiography) and 60 control non-diabetic subjects matched for age and sex, without visual or systemic symptoms. Contrast sensitivity was measured in spatial frequency of 3, 6, 12 and 18 cycles/degree (c/d). RESULTS: Contrast sensitivity was significantly lower (p < 0.001) in four spatial frequencies in all diabetic patients and in IDDM patients without retinopathy than in control group. Patients with IDDM and retinopathy had abnormal contrast sensitivity at spatial frequency 18 c/d when compared with patients without retinopathy. There was no correlation between contrast sensitivity and HbA1c values. CONCLUSIONS: Contrast sensitivity measurement in children and adolescents with type I diabetes is useful in the evaluation of the nature of early abnormalities in retinal function of diabetics.

Adolescent↗
PubMed1999

[Fluorescein angiography in children and adolescents with type I diabetes mellitus].

PURPOSE: The evaluation of changes in ophthalmoscopic examination and fluorescein angiography in children and adolescents with insulin dependent diabetes mellitus. MATERIAL AND METHODS: 100 patients with type I diabetes mellitus aged 9.3-21.5 years (15.76 +/- 2.69), with diabetes duration of 1-13.5 years (6.57 +/- 2.52) were examined. RESULTS: Retinal changes in ophthalmoscopic examination were observed in 12 cases. Fluorescein angiography allowed to detect diabetic retinopathy in 29 patients. There was no correlation between the incidence of retinopathy and the age of patients. Vascular abnormalities were related to the duration of diabetes. Retinopathy was not found in children < 10 years of age.

Adolescent↗
PubMed1999

[Helicobacter pylori as supposed factor of uveitis in children: a case report].

MATERIAL: A case of a 14-year-old boy with recurrent idiopathic bilateral uveitis. METHODS: Detection of Helicobacter pylori infection basing on endoscopic examination of upper alimentary tract and eradication of this factor using specific therapy leading to extinction of active uveitis in both eyes. RESULTS: Helicobacter pylori may be the etiological agent of uveitis in children with unknown reason of the disease.

Adolescent↗
PubMed1998

[The results of surgery on squint with pathological muscle attachment].

UNLABELLED: The aim of our study was to evaluate the results of the operation on squint with pathological attachment of lateral rectus revealed during surgery. MATERIAL AND METHODS: The study involved 22 children (12 girls and 10 boys) out of 320 children operated on for squint in the years 1993-1996, who had pathological attachment of lateral rectus. Alternating convergent squint was found in 12 children, unilateral in 10. Muscle exposure showed dislocation of lateral rectus attachment--upwards by c. 1.5 mm in 11 children, downwards and obliquely by c. 2 mm in 10 children, in 1 child the upper part of the attachment was shifted forward by 1 mm. During the surgery, in addition to the reduction of squint angle, physiological attachment of the rectus was reconstructed. RESULTS: The operation restored parallel position of the eyes in 22 children, very good vision was obtained in 12 (54%) with alternating convergent squint, significant improvement in 5 (22%) with unilateral convergent squint, and only slight improvement in the remaining 5 (22.7%) with high initial amblyopia and squint angle from +15 degrees to +30 degrees.

Child↗
PubMed1997

[Scanning electron microscopy (SEM) of subretinal fluid in idiopathic retinal detachment].

PURPOSE: To evaluate the cells of subretinal fluid (SRF) by the use of the scanning electron microscope and to determine relationships between morphologic features of SRF and duration of retinal detachment. MATERIALS AND METHODS: Subretinal fluid was taken from 7 patients with retinal detachment. The cytologic preparations were analyzed in electron scanning microscope (IEOL). RESULTS: The highest count of cells (predominantly macrophages and lymphocytes) were seen in SRF obtained in the third week after retinal detachment. Retinal detachment, which lasted more than 3 weeks, caused lower SRF cells count and changes in cells composition. After 6 months normal cells were not present in SRF.

Adult↗
PubMed1997

[Concentration of selected cytokines in subretinal fluid in patients with retinal detachment (IL-8, TNF-alpha, IFN-gamma)].

PURPOSE: The aim of this study was to evaluate the presence of IL-8, TNF-alpha, IFN-gamma in subretinal fluid of patients with rhegmatogenous retinal detachment. MATERIAL AND METHODS: We studied 15 patients operated on retinal detachment. The presence of cytokines was evaluated using immunoenzymatic assay. RESULTS: IL-8, TNF-alpha and IFN-gamma were found in all subretinal fluid samples. The concentration of cytokines in patients with retinal detachment lasting to 2 months was higher than in cases of retinal detachment over 2 months.

Adolescent↗
PubMed1997

[Levels of selected cytokines in aqueous humor of patients with cataract extraction].

AIM: The purpose of the investigation was to evaluate the presence of IL-1 beta, IL-2, IL-6, IL-8, TNF-alpha, IFN-gamma in aqueous humor of patients with cataract. MATERIALS AND METHOD: Aqueous humor was taken from 29 patients during extracapsular cataract extraction. The presence of cytokines was evaluated using immunoenzymatic methods. RESULTS: IL-2, IL-6, IL-8, TNF-alpha and IFN-gamma were found in all aqueous humor samples. There was no evidence for the presence of IL-1 beta. CONCLUSION: Cytokines may play role in the inflammation process after extracapsular cataract extraction.

Aged↗
PubMed1997

[Biological activity of subretinal fluid in selected in vitro tests].

PURPOSE: The aim of this study was to evaluate the ability of SRF to activate autologous leucocytes derived from the peripheral blood. MATERIAL AND METHOD: We studied 49 patients with idiopathic retinal detachment. Leucocyte migration inhibition, proliferation of T-lymphocytes after PHA stimulation, nonspecific suppressor activity induced by Con A, T-lymphocytes IL-2 production and basophils histamine release were assessed. RESULTS: Lymphocytes obtained from patients with retinal detachment lasting 3-4 weeks exhibited in vitro in the presence of SRF: increased ability to produce IL-2, increased proliferation after PHA stimulation and increased nonspecific T-suppressor activity induced by Con A. SRF enhanced also leucocyte migration inhibition and release of histamine from basophils.

Adult↗
PubMed1996

[Evaluation of posterior capsule opacification after extracapsular cataract extraction with and without implantation of intraocular lens].

PURPOSE: The aim of the study was evaluation of the rate of posterior capsule opacification after extracapsular cataract extraction with and without implantation of intraocular lens. MATERIAL AND METHOD: The clinical material comprised 77 patients operated on in the years 1990-1994. Only the cases in which visual acuity exceeded 5/10 were included into the study. 107 eyes were examined, among them 75 after implantation of posterior chamber intraocular lens. RESULTS: Opacification of the posterior capsule occurred in 37 eyes, but only in 25 of 75 eyes in the patients with implantation of the artificial lens. We found that following extracapsular cataract extraction without implantation of intraocular lens, opacification of the posterior capsule occurs more frequently, especially during 1-2 years after the surgery. CONCLUSION: Cortical-nuclear cataract and not much advanced age are factors predisposing for greater rate of opacification of the posterior capsule after extracapsular cataract extraction.

Adult↗
PubMed1996

[Surgery of orbital tumors with the Krönlein-Berke-Reese method in material from the Ophthalmology Clinics in Bałystok].

PURPOSE: To analyse the results of surgical treatment of tumors located in the posterior part of the orbit. MATERIAL AND METHODS: 12 patients (5 women and 7 men, aged 10-70 years) with tumors located in the posterior part of the orbit, operated on in the years 1985-1995 in Ophtalmology Clinic in Bialystok with the use of Krönlein-Berke-Reese lateral orbitotomy method. Tissues of removed tumor were evaluated histopathologically. RESULTS: In 7 cases tumor was removed completely, including 3 cases with simultaneous optic nerve removal. In 3 other cases tumor was removed partially, whereas in 2 cases eventration of the orbit was necessary. Persistent postsurgical defects of the eyeball mobility were not found in any of the patients. In 2 patients with partially removed tumor a constant blepharoptosis was observed, which required further operation. Visual acuity in patients with preserved optic nerve remained unchanged in 6 out of 7 cases. In 1 case-completely removed cavernoma-visual acuity decreased from 5/7 to 5/16. CONCLUSIONS: Lateral orbitotomy enables successful removal of tumors located in the posterior part of the orbit. Moreover, this method is relatively safe, especially in comparison with cranial approach to tumors badly located in the orbit.

Adolescent↗