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A Barkia

Publications and source records attributed to A Barkia.

27 records · Page 2Linked to original sources
PubMed1987

Relationship in adipose cells between the presence of receptor sites for high density lipoproteins and the promotion of reverse cholesterol transport.

The role of a high-affinity receptor site for high-density lipoproteins (HDL) has been investigated in parental Ob1771 adipose cells and their transformed counterparts after transfer of the complete early region of polyoma virus (Ob17PY cells). Binding of ApoAI, ApoAII and HDL3 occurs in Ob1771 cells and derived membranes, whereas no binding is observed in Ob17PY cells and derived membranes. After thymidine block, growth-arrested Ob17PY cells become able to bind ApoAI, ApoAII and HDL3; this recovery is prevented in actinomycin D- or cycloheximide-treated cells. In contrast to ApoAI, ApoAII or HDL3 binding, both growing and growth-arrested Ob17PY cells do show receptor activities for low density lipoproteins and transferrin, respectively, which are similar in affinity and maximal capacity. Following cholesterol accumulation which takes place in the presence of LDL cholesterol, subsequent exposure to HDL3 or ApoAI promotes cholesterol efflux from Ob1771 cells and growth-arrested Ob17PY cells but not from growing Ob17PY cells. These results show that the presence of a high-affinity receptor site for HDL in intact adipose cells is required for the promotion of reverse cholesterol transport.

Adipose Tissue↗
PubMed1987

Anion-exchange fast protein liquid chromatographic characterization and purification of apolipoproteins A-I, A-II, C-I, C-II, C-III0, C-III1, C-III2 and E from human plasma.

This paper describes a procedure for the rapid isolation of urea-soluble apolipoproteins (apo) from delipidated human very-low- and high-density lipoproteins using anion-exchange fast protein liquid chromatography. The separation was complete within 30 min and peaks corresponding to apolipoproteins A-I, A-II, C-I, C-II, C-III0, C-III1, C-III2 and E were identified by comparing their chromatographic, electrophoretic and immunological behaviour with that of purified standards of each protein. A second purification step is necessary to obtain pure apolipoproteins. Apo E, which is difficult to purify by conventional chromatography, has been obtained in a good yield. The apo C-II that was obtained produced a symmetrical peak on chromatography but three bands in isoelectric focusing. The method can be upgraded to a preparative scale and offers the possibility of direct purification of apolipoproteins both from high-density lipoproteins and (following preliminary gel chromatography) from very-low-density lipoproteins.

Anions↗
PubMed1987

[Urethral instability. Apropos of 26 cases].

The authors report their experience about 26 cases of urethral instability. This dysfunction is not rare; both sexes are involved, children was well as adults. Diagnosis is based on the data of simultaneous urethro-cystometry. Three clinical patterns are recognized: intermittent urinary leakage (6 cases), frequency and urgency (3 cases) and enuresis (17 cases). The urethral instability was pure in 13 cases and associated to detrusor hyperactivity in the other thirteen. But, in one of these the bladder instability was pure. A treatment based on alpha-adrenergic drugs has assured clinical relief and urodynamic stabilization of bladder and urethra.

Adolescent↗
PubMedDate not supplied

[Cystic pyelo-ureteritis apropos of 3 cases].

This anatomo-radiologic entity is rare. It presents diagnostic, pathogenic, therapeutic and prognostic problems which are discussed about three personal cases and the review of the literature.

Adult↗