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Biomedical subjects

A F Moroz

Publications and source records attributed to A F Moroz.

At least 19 recordsLinked to original sources
PubMed1991

[Serotypes of Pseudomonas aeruginosa strains isolated in obstetric institutions in Azerbaijan].

Examinations of Pseudomonas aeruginosa strains isolated from clinical material from newborns and puerperae and from the environmental objects (washings off, water) have shown that 45.45% of these strains belonged to serotype 04, 24.55% to serotype 02, and 16.38% to other serotypes. Serotype 04 was the most incident in all the tested samples, it was detectable in newborns' biotopes and on environmental objects. Serotype 02 strains ranked second in incidence; they were detected in clinical material from the newborns but not on environmental objects.

Azerbaijan↗
PubMed1991

[Treatment of endophthalmitis with a single intravitreous administration of cefotaxime and gentamicin in an experiment].

Experimental endophthalmitis was treated with single intravitreous administrations of cefotaxime (claforan) and gentamicin. It was found that a single administration of cefotaxime to the vitreous body prevented development of endophthalmitis in rabbits previously infected with Staphylococcus aureus, E. coli and Pseudomonas aeruginosa. Vitrectomy in the treatment of endophthalmitis was shown to be promising and provide satisfactory anatomical and functional results.

Animals↗
PubMed1991

[The therapeutic use of diucifon-activated immunocytes in adoptive transfer in mice with burn trauma complicated by Pseudomonas aeruginosa infection].

The effectiveness of the therapeutic use of diucyphone-stimulated syngeneic spleen cells was studied in mice with a burn trauma complicated by P. aeruginosa infection. For this purpose CBA mice were subjected to a burn of degree IIIa, covering 30% of their body surface, the wound was infected with P. aeruginosa strain, and 24 hours later spleen cells were taken from some of these animals. The spleen cells, incubated for 3 hours with or without diucyphone at a concentration of 10-100 micrograms/ml, were washed and reinfused intravenously to the remaining animals. The injection of diucyphone-treated spleen cells was shown to greatly increase the survival rate of the animals, to accelerate healing processes and to enhance the proliferative response of spleen cells to mitogens (phytohemagglutinin, concanavalin A) and interleukin-2. A conclusion was made on the efficacy of the therapeutic use of diucyphone-activated immunocompetent cells in cases of immunodeficiency induced by burn infection.

Adjuvants, Immunologic↗
PubMed1990

[Experimental development of aseptic adhesive films].

Antibacterial activity of surgical adhesive films containing various antiseptics in the adhesive layer was studied experimentally. It was shown that the adhesive films containing 0.1 to 1% (in terms of active iodine) of iodopyrone had a stable bactericidal action and were effective against to the main causative agents of surgical infections, i. e. Staphylococcus spp., E. coli, Klebsiella spp., Pseudomonas aeruginosa and others.

Anti-Infective Agents, Local↗
PubMed1990

[The diagnostic effectiveness of erythrocyte immunoreagents made from different Pseudomonas aeruginosa antigens].

The diagnostic effectiveness of the passive hemagglutination (PHA) tests with the use of erythrocyte diagnostica (ED based on exotoxin A (ETA) and poly- and monovalent ED based on lipopolysaccharides (LPS) isolated from 5 most widespread P. aeruginosa serogroups was compared. 97 patients with different purulent septic diseases and 100 practically healthy adult donors were examined. The treatment of serum samples with 2-mercaptoethanol decreased the diagnostic effectiveness of serological examination. The simultaneous determination of the activity of antibodies to ETA and LPS essentially increased the number of positive results of the PHA tests in patients with bacteriologically confirmed P. aeruginosa infection, but not in patients from whom other bacteria were isolated. Considering the sensitivity and specificity of serological examination, as well as the decrease of the volume of necessary work and the consumption of immunoreagents, the optimum test conditions proved to be ensured by the use of two ED: LPS-based polyvalent ED and ETA-based ED.

Antigens, Bacterial↗
PubMed1990

[The assessment of the toxigenicity of clinical strains of Pseudomonas aeruginosa by an immunoenzyme method using nitrocellulose filters].

The enzyme immunoassay (EIA) on nitrocellulose filters was adapted for the detection of exotoxin A in 104 P. aeruginosa strains isolated from patients and the environment in surgical wards in hospitals of Moscow and Alma-Ata. The method was shown to be highly sensitive: it permitted the detection of 5.ng of P. aeruginosa exotoxin A. The screening of 104 P. aeruginosa clinical strains by means of EIA on nitrocellulose filters revealed that these strains exotoxin A in 88.5% of cases.

Collodion↗
PubMed1989

[Use of a polyvalent erythrocyte diagnostic agent for determining antibodies to Pseudomonas aeruginosa in clinical practice].

The use of polyvalent erythrocyte diagnosticum prepared on the basis of 5 polysaccharide antigens of P. aeruginosa slime, isolated from strains belonging to the most widespread serovars, makes it possible to check up the humoral response of donors after their immunization with P. aeruginosa polyvalent corpuscular vaccine with the aim of obtaining anti-P. aeruginosa donor plasma. Antibody titers, determined in the passive hemagglutination test with the use of the proposed diagnosticum and corresponding to a serum dilution of 1:320 and greater, age tentatively diagnostic, which may be indicative of P. aeruginosa in the development of purulent septic complications in patients. The use of the passive hemagglutination test with the newly developed polyvalent erythrocyte diagnosticum makes it possible to check up the specific response of patients having P. aeruginosa infection in the process of their treatment with anti-P. aeruginosa hyperimmune plasma used as a part of complex therapy.

Antibodies, Bacterial↗
PubMed1989

[Erythrocyte diagnostic kits for detection of Pseudomonas aeruginosa exotoxin A].

The use of formulated chick red blood cells loaded with IgG preparations and affinity-purified antibodies, in comparison with initial immune serum to P. aeruginosa exotoxin A (ETA), has been shown to increase the sensitivity of antibody erythrocyte diagnosticum (AbED) 17-fold and to ensure the detection of ETA at a concentration of 1.2 mg of protein per ml. The passive hemagglutination (PHA) test with AbED has proved to be a more sensitive method for the detection of ETA than the antibody neutralization test with the use of antigenic erythrocyte diagnosticum, the latex agglutination test, the coagglutination test and the enzyme immunoassay. The PHA test has permitted the detection of ETA in the culture fluid of 80% of P. aeruginosa cultures under study.

ADP Ribose Transferases↗
PubMed1989

[Protective properties of anatoxin obtained from a homogeneous preparation of Pseudomonas aeruginosa exotoxin A].

The protective properties of formulated toxoid obtained from the highly purified preparation of P. aeruginosa exotoxin A have been studied in the test of the active immunization of mice. The study has revealed that the preparation when introduced in 1 or 2 injections in a dose of 15 micrograms, shows faint protective potency with respect to P. aeruginosa strains differing in virulence. Immunization with this toxoid in 3 and 4 injections has been found to ensure 60-100% and 50-60% protection of mice infected with P. aeruginosa toxigenic and proteolytic strains respectively. Immunization with toxoid has been found to induce the appearance of short-term antibacterial immunity which loses its capacity to protect the immunized animals, challenged with both toxigenic and proteolytic P. aeruginosa strains, as early as on day 28. The immunization of mice with toxoid in 4 injections has been shown to induce the development of antitoxic immunity capable of neutralizing up to 150 LD50 of purified exotoxin A.

ADP Ribose Transferases↗
PubMed1988

[Protective artificial polysaccharide-protein antigens made from the O-specific polysaccharides of Pseudomonas aeruginosa bacteria of the immunotypes 1, 2 and 7].

O-specific polysaccharide (PS) obtained from P. aeruginosa lipopolysaccharide (LPS), immunotypes 1, 2 and 7, were condensed in the presence of aminated bovine serum albumin (amBSA). As a result, artificial polysaccharide-protein antigens were synthesized and their protective properties were studied by the subcutaneous immunization of mice with their subsequent challenge with the cells of the homologous strain injected intraperitoneally in a dose of 3-5 LD50. The most effective immunization was achieved by using homologous LPS, taken in a subimmunogenic dose, as adjuvant. Heterologous LPS, taken in the same doses, showed no stimulating effect. The protective properties of the conjugates were also found to depend on the length of the PS chain. The conjugate of amBSA and PS of immunotype 2 with a molecular weight of about 10 KD was found to possess the highest protective potency. The decrease of the molecular weight of PS of immunotype 7 from 50 KD to 10 KD by selective acid hydrolysis led to obtaining the conjugate with high protective potency. The possibility of using the structurally linked pair antigen-adjuvant for the development of artificial vaccines is discussed.

Animals↗
PubMed1988

[Physico-chemical and biological characteristics of Pseudomonas aeruginosa elastase].

Elastase isolated from P. aeruginosa clinical strain hydrolyzes elastin, casein, hemoglobin, ovalbumin, gelatin, fibrin, collagen. The optimum pH ensuring the activity of the enzyme is 7.8-8.0. Elastase shows maximum stability at pH 6.6-9.0. Heating at 80 degrees C for 10 minutes results in its practically complete inactivation. Elastase is a highly radiosensitive enzyme. Chelating agents and zinc, cobalt, mercury ions suppress its activity. Sodium and ammonium chlorides selectively inhibit the elastolytic, but not proteolytic activity of the enzyme. Elastase shows pronounced dermonecrotic and keratolytic action.

Animals↗
PubMed1988

[Isolation and primary characteristics of elastase from a clinical strain of Pseudomonas aeruginosa].

The homogeneous preparation of elastase has been obtained from P. aeruginosa clinical strain. The molecular weight of the isolated enzyme is 33,000 daltons, and its isoelectric point is 6.8. Two media manufactured in this country (dialyzed bovine heart hydrolysate and a dried semisynthetic medium) ensuring good production of the enzyme have been proposed. The optimum time for the cultivation of the producer strain (30-40 hours) has been established. Elastase has been shown to be widely spread among P. aeruginosa clinical strains.

Animals↗
PubMed1988

[Production of an diagnostic erythrocyte agent from Pseudomonas aeruginosa exotoxin A].

The sensitization of formolized sheep red blood cells with exotoxin A by means of chromium chloride or glutaraldehyde is more effective with respect to their sensitivity in the passive hemagglutination test than loading by means of amidol, tannin and rivanol. The use of chromium chloride decreases the consumption of exotoxin A 2, 8, 16 and 16 times in comparison with the use of amidol, tannin, rivanol or glutaraldehyde respectively. The high specificity of erythrocyte diagnosticum obtained from exotoxin A by means of chromium chloride is indicated in the study of hyperimmune sera to 22 different antigens of enteric bacteria and staphylococci in the passive hemagglutination test and to 10 different enterobacterial and staphylococcal antigens in the antibody neutralization test.

ADP Ribose Transferases↗
PubMed1987

[Isolation of different variants of Pseudomonas aeruginosa anatoxin and their characteristics].

The conditions for the detoxification of the crude preparations of P. aeruginosa exotoxin A, obtained by the cultivation of strain PA-7 in Martin's broth, have been studied, and the schemes for obtaining nontoxic, stable, specifically antigenic preparations of toxoid from exotoxins A with different degrees of purification have been developed. Toxoid obtained by formalin treatment on the level of a crude preparation with its subsequent purification and additional detoxification with formalin in the presence of lysin has been shown to possess high immunogenic potency. The preparation has been found to induce immune response and to ensure the protection of experimental animals challenged not only with the lethal dose of exotoxin A, but also with P. aeruginosa toxigenic and protease-producing strains.

ADP Ribose Transferases↗
PubMed1987

[Immunoenzyme method of determining Pseudomonas aeruginosa exotoxin A by using a conjugate of beta-lactamase with specific antibodies].

Specific antibodies for immunoenzymatic assay of P. aeruginosa exotoxin A were isolated with affinity chromatography on exotoxin A-Sepharose 4B. A conjugate of the antibodies to exotoxin A with beta-lactamase from Bacillus licheniformis 749/c was prepared by linking glutaric aldehydes. The conjugate showed high immunospecific and enzymatic activity and was used for competitive solid phase immunoenzymatic assay. Sensitivity of the assay provided determination of exotoxin A in concentration of 20 ng/ml. It was used for determining P. aeruginosa exotoxin A in culture fluids.

ADP Ribose Transferases↗