[Study of the immune antibody spectrum in blood and plasma donors].
Explore the source record for details and available documents.
Antibodies, Bacterial↗
Biomedical subjects
A F Moroz
Publications and source records attributed to A F Moroz.
[Antibacterial activity of a number of preparations and their combination with a proteolytic enzyme against the microflora of burn and surgical wounds].
Antibacterial activity of antibiotics (azlocillin, tobramycin and piperacillin), antiseptics (metacid, chlorhexidine and quinosoline) and some other drugs (dioxidine and mafenide acetate) against the main representatives of the burn and surgical wound microflora, i. e. Staphylococcus, P. aeruginosa, Proteus, Providencia, Serratia, Klebsiella and E. coli was studied. Tobramycin and piperacillin were highly active against P. aeruginosa, Staphylococcus and Proteus. It was shown that in the presence of terrilytin, a proteolytic enzyme the activity of the combinations of chlorhexidine with dioxidine or mafenide acetate against all microorganisms occurring in burn and surgical wounds markedly increased.
Administration, Topical↗
[Effect of a corpuscular polyvalent Pseudomonas aeruginosa vaccine on the proliferation and differentiation of hematopoietic stem cells and erythropoiesis].
P. aeruginosa corpuscular polyvalent vaccine stimulates hematopoiesis in sublethally stimulated mice. The stimulating effect is dose-dependent. The most effective method of immunization is the intravenous injection of the vaccine. The degree to which the stimulation of hematopoiesis is manifested varies in different strains of mice. The stimulation of hematopoiesis is not linked with an increase in the content of erythropoietin. P. aeruginosa polyvalent corpuscular vaccine and monovaccine, prepared from P. aeruginosa strain 1313 and incorporated into the polyvalent vaccine, protect lethally irradiated mice in the postradiation survival test.
Animals↗
[Protective effect of a corpuscular polyvalent Pseudomonas aeruginosa vaccine in generalized chronic Pseudomonas aeruginosa infection in mice with cyclophosphamide-induced leukopenia].
The prophylactic effect of immunization with P. aeruginosa polyvalent corpuscular vaccine has been shown on the model of P. aeruginosa generalized chronic infection in mice with leukopenia induced by the intraperitoneal injection of cyclophosphamids. This effect is manifested by the increased resistance of the animals to sublethal doses of P. aeruginosa strain, as well as by more intense general and specific immunological responses in the infected animals (the increase of specific antibody titers, the number of leukocytes in the blood serum and the phagocytic activity of the cells of peritoneal exudate).
Animals↗
[Development of a latex agglutination method for the diagnosis of Pseudomonas aeruginosa infection].
The present investigation has revealed the possibility of using different kinds of monodispersed polystyrene latex, produced in the USSR, as carriers in the process of the preparation of antibody diagnosticums intended for the detection of water-soluble slime antigens of Pseudomonas aeruginosa strains belonging to the most widespread serological types. The optimum conditions for the preparation of latex reagents and for making the latex-agglutination test have been experimentally established. The new diagnosticums+ have been shown to be highly species- and type-specific, which permits making judgment on the presence or absence of slime antigens of P. aeruginosa strains belonging to definite serovars in the clinical material under study. The preparations thus obtained have been found to retain their sensitivity for 16 months (the term of observation).
Antibodies, Bacterial↗
[Hospital infection due to Pseudomonas aeruginosa in resuscitation departments].
The bacteriological survey of resuscitation and intensive care units has revealed the presence of P. aeruginosa strains in the microflora in 69.4% of cases. The circulation of 1-2 P. aeruginosa strains, identical in their serovar and pyocin type, is indicative of the presence of hospital infection and, therefore, the endogenous character of the contamination of patients. P. aeruginosa hospital strains are the main causative agents of infectious complications in the patients treated in resuscitation units.
Bronchi↗
[Development of a polyvalent erythrocyte diagnostic agent based on the antigens of Pseudomonas aeruginosa slime].
The study has revealed the fundamental possibility, and established the concrete conditions of obtaining standard erythrocyte polyvalent diagnosticum for the detection of antibodies to the antigens of P. aeruginosa, belonging to 5 types most frequently occurring in clinical practice, in the passive hemagglutination test. The data on high type- and species-specificity of the new erythrocyte diagnosticum have been obtained, which indicates that slime antigens have been correctly chosen as sensitins and confirms the necessity of using the polyvalent preparation. Preliminary data on the clinical trial of the erythrocyte diagnosticum show the expediency of its use for the control of specific immune response in donors in the process of obtaining anti-P. aeruginosa hyperimmune plasma, as well as the possibility of using this method for the serological diagnosis of P. aeruginosa infection. The diagnostic preparation has been found to retain its activity for 10 months (the term of observation).
Animals↗
[Possible use of plasmids for studying the effect of space flight factors on biological objects].
The action of space flight factors on the phenotype and certain molecular and genetic parameters of E. coli plasmids (R, Col, Hly and others) was studied. The E. coli strains were grown and multiplied in the "Cytos" apparatus during the orbital flight of "Salyut-7" in June 1982. A synchronous experiment in the "Cytos" apparatus was performed under the Earth conditions. It was shown that space flight factors had no effect on the properties of the test strains and plasmids (antibiotic resistance, capacity for production of colicin, hemolysin, restriction endonuclease, conjugative capacity, frequency of conjugative transfers and molecular weight).
Anti-Bacterial Agents↗
[Infectious complications and hospital infection in the resuscitation and intensive therapy departments].
Explore the source record for details and available documents.
Cross Infection↗
Preliminary results of Cytos 2 experiment.
Cytos 2 experiment, carried out during the French-Soviet manned flight (July 1982), has studied the antibiotics sensitivity of bacteria cultivated in vitro during the orbital flight. The results show an increase of the antibiotics resistance and a larger thickness of the cellular envelope for the inflight cells. The increase of antibiotics resistance can be related to a stimulating effect of space on the cell growth rate or to changes of the cellular envelope structure.
Anti-Bacterial Agents↗
[Protective action of a corpuscular polyvalent Pseudomonas aeruginosa vaccine against representative enterobacteria].
Animal experiments have demonstrated that P. aeruginosa vaccine is capable of protecting animals from experimental P. aeruginosa infection, as well as rendering a protective effect with respect to some representatives of the family Enterobacteriaceae. The comparative study of the antigenic spectra of the vaccine strains and some representatives of Enterobacteriaceae (Enterobacter, Serratia, Citrobacter and Klebsiella) has revealed no direct relationship between the degree of this protective effect and the presence of common antigenic determinants in them.
Animals↗
[Effect of R plasmids on the growth characteristics of Shigella sonnei and Escherichia coli].
Revealing of growth characteristics in plasmid and plasmid-free strains was studied with the use of two different hosts: E. coli 15-3 and Sh. sonnei 11-941 containing conjugative R plasmids differing in the set of the resistance markers. It was shown that the R plasmids had no noticeable effect on the period of the lag phase and the time of the microbial cell generation. It was also shown that the number of the viable cells in separate cultures of the plasmid-free strain of Sh. sonnei 11-941 and its plasmid variants was of the same order. Counting of the viable cells in mixed cultures of the plasmid-free strain of E. coli 15-3 and its plasmid variant on the complete nutrient medium revealed an insignificant increase (by 10 per cent) in the proportion of the bacteria carrying the plasmid after 6-hour growth (during early stationary growth phase) and later, up to 24 hours. The results of the study suggested that development of nutrient media for microbial strains containing R plasmids does not require additional cultivation conditions.
Cell Division↗
[Effectiveness of a polyvalent corpuscular Pseudomonas aeruginosa vaccine, antibiotics and their combinations in experimental chronic Pseudomonas aeruginosa infection].
The data on the development of the experimental model of P. aeruginosa chronic infection in mice, produced by their intraperitoneal inoculation with the infective agent, and on the study of the properties of this model are presented. The model has been used in the experimental study of the preventive action of P. aeruginosa polyvalent corpuscular vaccine. The comparative study, carried out with the use of the proposed model, has been made with a view to evaluating the effectiveness of different methods for the treatment of P. aeruginosa chronic septic infection by means of antibiotics (polymixin B and tobramycin), P. aeruginosa polyvalent corpuscular vaccine and their combination. The combined use of this vaccine with antibiotics (polymixin B or tobramycin) has proved to give the most pronounced curative effect with respect to P. aeruginosa chronic infection.
Animals↗
[Isolation and study of the properties of a polyvalent corpuscular Pseudomonas aeruginosa vaccine. An evaluation of the biological properties of a polyvalent corpuscular Pseudomonas aeruginosa vaccine].
P. aeruginosa killed polyvalent corpuscular vaccine, tested in a trial on 42 volunteer donors, is safe, low reactogenic and possesses pronounced immunogenicity, as the injection of the vaccine induced a rise in the titer of antibodies to P. aeruginosa up to 1:1280 in 95% of the immunized donors. To obtain specific antibodies in the blood plasma of the donors in an amount sufficient for the protective activity of the plasma becoming manifest, it is expedient to use the vaccination schedule providing for subcutaneous injections of 0.5, 0.5 and 1.0 ml at intervals of 7 days. Intense immunity thus induced in the donors lasts for 3-4 months. The hyperimmune plasma obtained from the donors has an antibody titer of at least 1:320 and shows a 90-100% protective effect in mice infected intraperitoneally with P. aeruginosa. The preliminary results of the clinical trial of anti-P. aeruginosa plasma have demonstrated its efficiency as a part of the complex treatment of patients with purulent septic complications of P. aeruginosa etiology.
Adult↗
[Biological characteristics of bacteria during the Cytos-2 Soviet-French space experiment].
The experiment "Cytos-2" made it possible to reveal a certain increase in the antibiotic resistance of bacteria constituting the opportunistic pathogenic microflora of man (Staphylococcus, Escherichia coli and Pseudomonas aeruginosa) to oxacillin, erythromycin, colistin and kanamycin under the conditions of space flight. The electron-microscopic study of the postflight staphylococcal cultures revealed the thickening of the cell membrane in staphylococci. The conditions of the experiment did not affect the stability of the biochemical characteristics of P. aeruginosa cultures, as well as their virulence, immunogenic properties and type specificity.
Anti-Bacterial Agents↗
[Production and study of the properties of a polyvalent corpuscular Pseudomonas aeruginosa vaccine. IV. The effect of immunization with Pseudomonas aeruginosa vaccine on the immune status of volunteer donors].
The influence of immunization with P. aeruginosa vaccine on the immune status of volunteer donors has been studied. Immunization with P. aeruginosa vaccine in doses of 0.5-0.5-1.0 ml at intervals of 7 days has been found to lead to the 13-fold increase of the titer of specific antibodies, lasting for 3-4 months, which ensures the possibility of obtaining anti-P. aeruginosa hyperimmune plasma with standard titers. The injection of P. aeruginosa vaccine to donors leads to the activation of humoral immunity simultaneously with the increase of the absolute and relative number of rosette-forming B-lymphocytes without essential changes in the amount of rosette-forming T-lymphocytes.
Adult↗
[Antibiotic resistance of hospital strains of Pseudomonas aeruginosa in resuscitation and intensive therapy units].
Explore the source record for details and available documents.
Anti-Bacterial Agents↗
[Exotoxin A production during Pseudomonas aeruginosa PA-7 cultivation in Martin's broth].
The activity of exotoxin A in culture filtrates prepared from cultures obtained by growing P. aeruginosa strains PA-7 and PA-103 in Martin's broth containing iron at a concentration of 0.08 microgram/ml, 0,05 M sodium glutamate and 1% of glycerin has been shown to be 1.5 times higher than that in filtrates prepared from cultures obtained by growing the above strains in a medium containing soybean tryptic digestion (USA). The optimun conditions for the production of exotoxin A by these strains are achieved during their cultivation in a fermenter at a temperature of 32 degrees C for 18 hours with simultaneous stirring (800 r. p. m.) and oxygenation (450 m3/h). Under these conditions the biological activity of the filtrates is 200 LD50/ml, their ADP-ribosyltransferase activity is 9500 c. p. m. and a sharply defined precipitation line appears in the double diffusion test in gel with monospecific antiserum to purified toxin, used in a dilution of 1:8.
ADP Ribose Transferases↗