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Biomedical subjects

A Ferencz

Publications and source records attributed to A Ferencz.

50 records · Page 3Linked to original sources
PubMed1979

Development of specific cellular immunoreactivity in typhoid fever.

Development of cellular immunoreactivity to Salmonella typhi and Salmonella paratyphi-A was studied by the leukocyte migration inhibition test in 9 patients with typhoid fever and in 2 patients with paratyphoid fever. Cellular reactivity could be demonstrated from the first days of the disease in all the subjects. The most pronounced migration inhibition was observed during the febrile period. It is suggested that specific cellular reactivity may play a pathogenetic role in typhoid fever.

Adult↗
PubMed1978

Neurological infections caused by herpes simplex virus in adults.

Over a period of 33 months, 281 neuro-infections were tested for herpes simplex virus. In 26 cases herpes simplex virus was identified as the aetiological agent by isolation of the virus from the cerebrospinal fluid and by serological tests. The illness took the form of severe encephalitis in four cases, less severe encephalitis or meningoencephalitis in 12 cases, aseptic meningitis in seven cases, meningo-encephalitis with radiculitis in one case, ascending radiculomyelitis in one case and radiculitis in one case. The diagnostical and therapeutical problems of herpes simplex neuro-infections are discussed.

Adolescent↗
PubMed1976

Effect of whole-body irradiation on the synethesis of ribonucleic acid associated with nuclear ribonucleoprotein particles of rat liver.

Synthesis of the RNA of rat liver nuclear RNP particles ("informofers") was studied within 12 hrs after 1930 rad whole-body gamma-irradiation. 14C-orotic acid was administratered intravenously and nuclear RNP particles were extracted by 0.1 M and by 0.3 M salt solutions at pH 8.0. Radioactivity of the RNP particles 1.5--2.0 times higher than that of the unirradiated controls up to 6 hrs after irradiation, exhibiting a maximum at the first hour. The labelling of the 0.3 M RNP particles was higher and chased less rapidly than that of the 0.1 M RNP particles. The RNA to protein ratio and the protein composition of the RNP the RNP particles did not change after irradiation as examined by CsCl density gradient centrifugation and by acrylamide gel electrophoresis, respectively. In the cytoplasm enhanced synthesis of rapidly labelled RNA sedimenting with 40 S cytoplasmic RNP paricles could also be demonstrated after irradiation. It is concluded that the synthesis of messenger-type RNA associated with liver nuclear RNP particles increases and more RNA is transferred from the nucleus to the cytoplasm after whole-body irradiation.

Animals↗
PubMed1975

Comparative effect of heparin on RNA synthesis of isolated rat-liver nucleoli and purified RNA polymerase A.

The polyanion heparin has been employed to study the interaction of rat liver DNA-dependent RNA polymerase A and its template under various conditions. Heparin very efficiently inhibits polymerase molecules, which are not bound to DNA or are associated with the template in a loose, i.e. non-specific fashion. Purified nucleoli, isloated from rat liver nuclei, contain RNA polymerase A in abundant quantities of which only a portion is bound in a transcriptional complex. Excess enzyme, which is contained in the nucleolus in a quasi free form, can be transferred to an exogenously added template and can be completely inhibited by the prior addition of heparin. The enzyme contained in a transcriptional complex, however, initiated in vivo and completing these RNA chains in vitro, is fully resistant to heparin. In contrast to these results it has been found that RNA polymerase A extracted from nuclei and purified by various chromatographic steps does not form heparin-resistant complexes, even after the enzyme has been bound to the DNA template. Moreover it has been found that purified RNA polymerase A transcribes truly native DNA extremely poorly, indicating that the enzyme is highly deficient in the act of initiation on duplex DNA. It is therefore questionable whether the interaction of the purified enzyme and isolated DNA represents binding to true initiation complexes as is observed in the intact nucleolus.

Animals↗
PubMedDate not supplied

Threshold level of NF-kB activation in small bowel ischemic preconditioning procedure.

Ischemic preconditioning (IPC), which is obtained by exposure to brief periods of vascular occlusion, improves organ tolerance to prolonged ischemia. The aim of this study was to evaluate the threshold level of NF-kB activation in small intestine during an IPC procedure. Various intestinal IPC were performed on 20 Wistar rats in seven groups: group I (GI, nonpreconditioned); group II (GII, 1-minute ischemia and 1-minute reperfusion); group III (GIII, two cycles of 1-minute ischemia and 1-minute reperfusion); group IV (GIV, 2-minutes ischemia and 2-minutes reperfusion); group V (GV, two cycles of 2-minute ischemia and 2-minute reperfusion); group VI (GVI, 5-minute ischemia and 10-minute reperfusion); group VII (GVII, two cycles of 5-minute ischemia and 10-minute reperfusion). Bowel biopsies were collected after laparotomy (control) as well as at 30, 60, and 120 minutes following IPC. We determined the cytoplasmic and nuclear NF-kB by a chemiluminescence-based ELISA method. Our results showed low, constant NF-kB levels in GI. In the preconditioned groups (GII-GVII), NF-kB was significantly elevated at 30 minutes following IPC (P < .05 vs control). After 1 hour, NF-kB activity decreased to the control level. However, 2 hours after IPC both forms of NF-kB were elevated significantly again, which was independent of the number of IPC cycles (P < .05 vs control). Our experiments revealed that one cycle of 1-minute ischemia and 1-minute reperfusion is a critical threshold level for NF-kB activation during small bowel IPC. Longer and more IPC cycles did not result in further elevation of NF-kB activation.

Animals↗