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Biomedical subjects

A Ferraro

Publications and source records attributed to A Ferraro.

At least 37 records · Page 2Linked to original sources
PubMed1989

Tissue specificity of chromatin glycoproteins recognized by concanavalin A.

Chromatin glycoproteins recognized by Concanavalin A have been isolated from pig liver, kidney and heart by the use of immobilized lectin. Two groups of proteins differing in affinity for DNA have been analysed. Glycoproteins are mainly present in the group of proteins which are tightly bound to DNA. Mono and bidimensional electrophoretic patterns of total tightly bound proteins reveal a similarity among the three organs examined, while the corresponding patterns of the glycoproteins are typical for each organ. The tissue specificity of chromatin glycoproteins, together with their capability to interact not only with DNA but possibly also with other nuclear components, suggest a role for these proteins in the mechanism of genome expression.

Animals↗
PubMed1988

Calorimetric analysis of sodium dodecylsulfate-chromatin interaction.

Microcalorimetric titrations of whole chromatin and histones with sodium dodecylsulfate were performed at pH 7 and 25 degrees C. Enthalpy variations at low detergent concentration (less than 0.02%) are much more negative for histones than for chromatin. At 0.065% sodium dodecylsulfate the difference between the two curves becomes constant and, after correction for monomerization effects, amounts to +130 kcal/mol of nucleosomal unit. Core particles show heat effects similar to those of histones. These findings suggest that the chromatin structure is not stabilized exclusively by electrostatic interactions and that hydrogen bonds responsible for the additional stability may be contributed by non histone chromatin proteins.

Animals↗
PubMed1986

Thiol proteins in chromatin.

Total half-cystine residues in proteins of pig liver chromatin have been measured. About half of them are present in the reduced state. Thiol groups of non-histone chromatin proteins, which amount to about 40 nmol/mg of protein, are preferentially located in chromatin fragments which are more easily solubilised either by DNAse I or by DNAse II. The data obtained are compatible with an involvement of SH and SS groups in chromatin structure and function.

Amino Acids↗
PubMed1986

[Computerized tomography in spinal osteoblastoma. Observations in 10 cases].

In order to stress the role of computed tomography (CT) in the evaluation of benign spinal osteoblastoma, the authors examined by CT 10 patients with surgically and histologically proved osteoblastomas of the spine. Basing on surgical and histopathological findings CT proved to be a very important method for attesting the presence of the tumors (usually showed by radiographic examination), and specially for their staging and follow-up. CT in fact showed the real extent of the tumor into the spinal canal and towards the paraspinal soft tissues, and allowed the early detection of post surgical relapses. The authors conclude that CT is a primary diagnostic modality for preoperative planning and follow-up of benign osteoblastoma.

Adolescent↗
PubMed1984

The interaction of bisbenzimide with DNA.

The interaction of bisbenzimide (Hoechst 33258) with DNA has been studied by microcalorimetry, fluorimetry and fluorescence polarization measurements. The binding does not involve intercalation, takes place in at least two modes and occurs in the major groove at low dye/DNA molar ratio (first mode of binding), in agreement with previous proposals by other Authors. Furthermore it has been shown that i) at high dye/DNA molar ratio the binding (second mode) occurs in both grooves; ii) at pH 7.0 the protonation of a dye nitrogen accompanies the binding in both modes; iii) the quantum yield of the dye-DNA complex is sensitive to the DNA conformation change caused by an increase in ionic strength. Basically the same type of binding takes place on chromatin, as detected by calorimetry. However, at high dye/DNA molar ratio about 30% of DNA is not accessible to the dye. The decreased accessibility appears to be mainly related to the nucleosomal structure of chromatin.

Animals↗
PubMedDate not supplied

Bone tumors of the foot: epidemiology and diagnosis.

The authors reviewed 260 cases of bone tumors localized in the foot and treated at the Tumor Center of the Rizzoli Orthopaedic Institute: 191 were benign and 58 malignant and 11 metastasis. There was predilection for the hindfoot (57%), followed by the forefoot (33%) (prevalently the metatarsals). Localizations in the midfoot were rare (10%). Osteoid osteoma was observed in 26% of all of the tumors of the foot and in 35% of the benign forms. Among malignant neoplasms Ewing's sarcoma (27.5%) was the most frequent. Conservative surgery, which was always carried out in benign tumors, was also performed in some of the malignant ones, having an early diagnosis and a correct preoperative study. The indications and the limits of the different imaging techniques available are reported (bone scan, arteriography, CAT, MRI), and the role of biopsy in the definitive diagnosis of these neoplasms in discussed.

Adult↗
PubMedDate not supplied

Supracondylar process of the humerus: four cases.

Four cases of supracondylar process of the humerus are presented. Two of the patients were asymptomatic and in one of these the supracondylar process was fractured. In a third there was median nerve compression and in the last there was ulnar nerve entrapment. The anatomical features, clinical symptoms and therapy are described. Good results were obtained after resection of the supracondylar process; neurological symptoms regressed one month after surgery.

Adult↗
PubMedDate not supplied

Fucose-carrying nuclear glycoproteins: distribution and tissue specificity.

Glycoproteins recognized by Ulex europaeus lectin I, specific for fucose residues, have been identified in chromatin prepared from pig liver, heart and kidney nuclei. They are present among the proteins dissociated from DNA with salt solutions and, in higher amount, among the proteins dissociated with urea and guanidine. In any case these fucose-containing glycoproteins appear to have a marked tissue specificity, which suggests that they have specific regulatory roles in the processes taking place in the nuclei.

Animals↗
PubMedDate not supplied

Chondrosarcoma of the hand and foot.

Among possible localizations of chondrosarcoma of the limbs, the hand and foot represent the rarest sites. Five-hundred six cases of chondrosarcoma have been observed at the Tumor Center of the Rizzoli Orthopaedic Institute. Of these, 4 were localized in the hand and 10 in the foot. The authors compared their cases with those reported in the international literature. The authors emphasize the particular aspects of the tumor when localized in these sites which may be responsible for late diagnosis and difficulty in differential diagnosis involving synovial chondromatosis and, above all, chondroma, from which chondrosarcoma may originate.

Adult↗
PubMedDate not supplied

3'-5' cAMP phosphodiesterase in pig liver nuclei.

cAMP phosphodiesterase was partially purified from pig liver nuclei and some of its properties were investigated. The enzyme is a low km PDE. Inhibition by xanthine derivatives is in line with the behaviour of PDE from other mammalian sources, while small differences are observed towards nucleotides inhibitors. The enzyme is a chromatin-bound protein although no specific location related to transcriptional function could be demonstrated.

3',5'-Cyclic-AMP Phosphodiesterases↗