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Biomedical subjects

A Forsgren

Publications and source records attributed to A Forsgren.

At least 109 records · Page 6Linked to original sources
PubMed1987

Leukocyte migration in vivo and in vitro in patients with psoriasis.

Leukocyte migration in vivo was studied with a skin chamber technique in 21 patients with active psoriasis vulgaris and 18 with cleared psoriasis vulgaris. Measuring over 24 h, no difference was found between healthy volunteers and most patients with active psoriasis, although a subgroup of patients with long-lasting relapses showed subnormal migration values. In patients with cleared psoriasis on the other hand the in vivo leukocyte migration values were increased. In addition, leukocyte migration in vitro under agarose was studied, but no difference was found between healthy controls and patients with psoriasis, active or cleared.

Adult↗
PubMed1986

Antibodies reactive with the B1 molecule inhibit cell cycle progression but not activation of human B lymphocytes.

The B1 cell surface molecule (CD20) is a 35-kDa phosphoprotein expressed by B lineage cells during most stages of differentiation. Some monoclonal antibodies reactive with B1 induce activation while others, anti-B1a, inhibit B lymphocyte function. To further determine the requirement of B1 molecule function in proliferation and differentiation the effects of anti-B1a antibody binding on early cellular activation events were examined. Immunoglobulin secretion of lymphocyte cultures stimulated with pokeweed mitogen was maximally inhibited if the antibody (1-10 micrograms/ml) was added during the first 24 h of culture. However, even high antibody concentrations were unable to inhibit increases in free intracellular Ca2+ concentrations immediately following cross-linkage of cell surface immunoglobulin, or inhibit cell enlargement and the expression of transferrin and interleukin 2 receptors. Antibody binding to B1 inhibited RNA synthesis (37-80%) and progression through cell cycle following activation. In contrast, proliferation induced by phorbol myristate acetate was not inhibited by antibody binding to the B1 molecule. The findings that the earliest activation events and phorbol myristate acetate-induced proliferation were not inhibited by antibody binding to B1 suggest that inhibition is due to the blocking of a step of the activation process required for cell cycle progression and differentiation, rather than blocking initial signal transduction across the membrane or providing a negative or suppressive signal.

Antibodies, Monoclonal↗
PubMed1986

The immunosuppressive effect of streptozotocin-induced diabetes in rats.

Streptozotocin-diabetic isogenous Brown-Norway (BN) rats received heterotopic Wistar-Furth (WF X BN) F1 heart transplants. The functional graft survival time after different duration of diabetes and during insulin-treated diabetes was recorded. Some diabetic rats were challenged with sheep red cells, and their spleens were used in PHA stimulation and plaque-forming assays. Prolongation in heart transplant survival was found to be independent of the duration of diabetes. The prolongation disappeared promptly when insulin was administered to the diabetic rats. A depression of cellular immunoresponsiveness as measured by PHA stimulation assay was recorded during diabetes. Valid results were not obtained in the plaque-forming assay.

Animals↗
PubMed1986

Faecal carriage of group B streptococci.

A study of 1,138 primarily healthy subjects of various ages and sex was conducted to determine the faecal isolation rate of group B streptococci. Five percent of 284 neonates (less than or equal to 5 days old) and 4% of 267 healthy children (1-15 years old) were found to be faecal carriers. Adults were more frequently faecal carriers than children, group B streptococci being isolated in 15% of 361 adults and 11% of 226 pregnant patients. The isolation rate was independent of sex at all ages. Although group B streptococci were found more frequently in rectal than in faecal specimens from pregnant women (p less than or equal to 0.001), the isolation rate for faecal specimens could be increased by using a more selective broth. Forty-four percent of strains isolated from faeces of 105 subjects belonged to serotype III, 27% to type Ia, 15% to type Ib, 11% to type II and 3% were nontypeable. The same serotype of group B streptococci was usually present at different sites in each subject.

Adolescent↗
PubMed1986

Quinolones affect thymidine incorporation into the DNA of human lymphocytes.

The incorporation of [3H]thymidine into DNA was increased in phytohemagglutinin-stimulated human lymphocytes exposed to four of the new quinolone derivatives (ciprofloxacin, norfloxacin, ofloxacin, and A 56620) at concentrations achievable in clinical situations. However, proliferation of phytohemagglutinin-stimulated lymphocytes was not influenced by ciprofloxacin at concentrations of 0.5 to 10 micrograms/ml.

DNA↗
PubMed1986

Group B streptococci at delivery: high count in urine increases risk for neonatal colonization.

Of 858 pregnant women studied in matched rectal, urethral and urine cultured specimens, 186 (22%) were found to be colonized by group B streptococci (GBS). GBS were detected significantly more often in rectal specimens (159) than in urethral specimens (108) or in urine specimens (64). This is supporting evidence for the gastrointestinal tract as the main habitat of GBS. Of 1786 women whose urine was sampled at delivery, GBS were isolated from 128 (7%), in 22 of whom (1% of the total) GBS were present in quantities greater than or equal to 10(4) colony forming units (cfu)/ml urine. Neonates born to women with greater than or equal to 10(4) cfu GBS/ml urine were apparently at greater risk for neonatal infection, as they were more commonly and more heavily colonized than were the newborns of women with lower quantities of GBS in urine, or if positive urethral or rectal specimens were considered. The incidence of preterm delivery or obstetric infection was not higher among women in whom GBS were isolated in specimens from any of the 3 sites; foetal distress was more common among their children, but not neonatal respiratory or infectious diseases of which the incidence was low and difficult to assess statistically.

Bacteriuria↗
PubMed1986

The effectiveness of a short perioperative course with pivampicillin/pivmecillinam in transurethral prostatic resection: clinical results.

In a randomized control study comprising 261 patients undergoing transurethral prostatic resection (TUR), the effect of a short perioperative course with the oral combination of pivampicillin/pivmecillinam (PAPM) was analysed in 129 patients. The study was divided in 2 parts: the first 60 patients received 450 mg and the following 69 patients, 900 mg every 12 h until removal of catheter but not longer than for 1 week. 132 controls received parenterally 1 g of cefotaxime (CFT) daily throughout the study. During the first part of the study the frequency of bacteriuria in the PAPM group was 43% preoperatively and 30% 10 days postoperatively, during the second part 47% and 12%, respectively (p less than 0.025). In the CFT group the frequency of bacteriuria was reduced from 52% preoperatively to 28% postoperatively. The prophylactic effect (i.e. the protection against acquired bacteriuria) was 96% and 92% in the PAPM and the CFT groups, respectively. Preoperative bacteriuria was eliminated in 40% of the patients during first part and 69% during second part in the PAPM group, while corresponding figures were 48% and 46% in the cefotaxime group. There were 2 cases of septicemia and 5 of upper urinary tract infections throughout the study evenly distributed between the two groups. Oral pivampicillin/pivmecillinam 900 mg every 12 h was found to be a good alternative for perioperative antibiotic prophylaxis at TUR.

Administration, Oral↗
PubMed1986

The effectiveness of a short perioperative course with pivampicillin/pivmecillinam in transurethral prostatic resection: bacteriological results.

We analysed the bacteriological findings in 261 patients undergoing transurethral prostatic resection (TUR) and receiving either an oral course of pivampicillin/pivmecillinam (PAPM) or parenteral cefotaxime (CFT) in a randomized clinical trial. 123/261 patients had bacteriuria before TUR; 80% of the bacteria were gram-negative strains and 20% gram-positive. 88% of the strains were sensitive to PAPM and 93% to CFT but only 58% to ampicillin. The sensitivity of recurring bacteria was not influenced by the short course of PAPM or CFT. The faecal flora was influenced by the treatment with PAPM in terms of growth of Pseudomonas aeruginosa and fungi in some patients, but no resistant strains of Enterobacteriaceae were observed. The peak serum concentrations of ampicillin and mecillinam were obtained 2 hours after intake of the drug and were 4.5 micrograms/ml and 1.7 micrograms/ml respectively. The prostate tissue concentration of ampicillin and mecillinam (AM) was low.

Administration, Oral↗
PubMed1986

In vitro aminoglycoside resistance of gram-negative bacilli and staphylococci isolated from blood in Sweden 1980-1984.

The in vitro susceptibility to gentamicin, tobramycin, amikacin and netilmicin in septicaemia isolates was followed during 1980-1984 in 6-8 Swedish laboratories. The bacterial distribution was similar over the years and was dominated by Escherichia coli and staphylococci. Resistance to gentamicin was found in 2.3-3.6%, to tobramycin in 1.4-3.4%, to amikacin and netilmicin in 0.5-0.9%. Production of aminoglycoside modifying enzymes was observed among resistant strains.

Amikacin↗
PubMed1985

Antibiotic treatment during surgery for diffuse peritonitis: a prospective randomized study comparing the effects of cefuroxime and of a cefuroxime and metronidazole combination.

In a prospective randomized open study of patients operated upon for diffuse peritonitis, the effects of two different antibiotic regimens were evaluated. Cefuroxime given as a single drug (Group I; n = 59) was compared with a combination of cefuroxime and metronidazole (Group II; n = 63). Bacteriological cultures, both aerobic and anaerobic, were obtained peroperatively and in the event of any complication. The antibiotic sensitivities of isolated bacteria, and the serum and tissue concentrations of cefuroxime were determined. Postoperative infectious complications occurred in 22 per cent of Group I patients (cefuroxime), and in 17.5 per cent of Group II (cefuroxime plus metronidazole). The mortality rates were 5 per cent for Group I and 8 per cent for Group II. Tissue concentrations of cefuroxime were well above the MIC (minimal inhibiting concentration) values for most of the bacteria isolated. From a few patients in Group I, however, cultures were obtained with isolates sensitive to metronidazole but resistant to cefuroxime. Our findings suggest that, in the antibiotic treatment of patients operated for diffuse peritonitis, an agent which is primarily effective against aerobic bacteria (but not entirely without effect on anaerobes) is as effective as combination therapy covering both aerobic and anaerobic bacteria.

Adolescent↗
PubMed1985

Effect of ciprofloxacin on phagocytosis.

Certain aspects of the relationship between host defence mechanisms and the new quinoline derivative ciprofloxacin in comparison to norfloxacin and ofloxacin were studied. Ciprofloxacin did not affect chemotaxis of human polymorphonuclear leucocytes in agarose. In leucocytes exposed to ciprofloxacin, norfloxacin and ofloxacin neither the chemiluminescent response to opsonized zymosan and formyl-methionyl-leucyl-phenylalanine nor the phagocytic or bactericidal activity was affected. However, killing of Staphylococcus aureus by human polymorphonuclear leucocytes exposed to subinhibitory concentrations of ciprofloxacin was enhanced. The results show that the quinolines tested do not directly influence phagocytic cells, but a subinhibitory concentration can make bacteria more susceptible to phagocytosis and killing.

Chemotaxis, Leukocyte↗
PubMed1985

Impact of short perioperative courses of cefotaxime on aerobic bacterial flora in patients undergoing transurethral prostatic resection.

The influence of short-term prophylactic courses of cefotaxime on the microbial environment was studied. The distribution of bacterial species and their antibiotic resistance was recorded in isolates collected over a six-year period, during which time cefotaxime was used for perioperative prophylaxis in patients undergoing transurethral prostatic resection. In three consecutive studies covering the six-year observation period, the species distribution and antibiotic resistance patterns remained essentially unchanged for both cefotaxime and ampicillin. Examination of the faecal coliform flora in 23 patients given cefotaxime revealed no marked qualitative or quantitative change in the flora. It is concluded that short-term prophylactic courses of cefotaxime do not promote the emergence of resistance in the aerobic bacterial flora.

Aged↗
PubMed1985

Experience with a skin chamber technique for leucocyte migration studies.

An in vivo skin chamber method using lesions obtained by suction was evaluated. Neither dyspigmentation nor scarring were seen after 2 mth. The number of leucocytes accumulated in the collection chamber was correlated to the area of the lesion. Reproducibility remained essentially unchanged over an extended period and was 19% for one chamber and 13.6% for determinations with two chambers. No correlation was found between results obtained with the skin chamber technique and with chemotaxis or random migration as determined by an underagarose technique. When factors influencing in vivo and in vitro migration were studied, it was found that nonsteroidal anti-inflammatory drugs when given to arthritis patients or healthy volunteers inhibit leucocyte migration in vivo while in vitro migration was unchanged. Activated serum and LTB4 attracted leucocytes both in vivo and in vitro. The attraction by serum appeared at least partly to be caused by C5a. Polymorphonuclear leucocytes harvested from skin chambers were chemotactically deactivated and their bactericidal capacity reduced. The chemiluminescent response to formyl-methionyl-leucyl-phenylalanine and opsonized zymosan was increased. Exposed to zymosan activated serum, blood leucocytes showed a similar functional modification as leucocytes harvested from a skin chamber, and our findings suggest that the altered function of leucocytes in an inflammatory focus is largely the result of their exposure to chemotactic factors.

Anti-Inflammatory Agents↗
PubMed1985

A skin chamber technique for leukocyte migration studies; description and reproducibility.

An in vivo skin chamber method, using lesions obtained by suction, was evaluated for studying leukocyte migration. No dyspigmentation or scar was seen after two months. The number of leukocytes accumulated in the collection chamber was 6.9 X 10(7)/cm2 and was correlated to the area of the lesion (r = 0.964). Reproducibility, essentially unchanged over an extended period, was 19% for one skin chamber and 13.6% for determinations with duplicate chambers; by comparison, with an under-agarose technique, the coefficient of variation for migration was low on consecutive days (6%), but much higher (29%) when determined over a longer period. No correlation was found between the skin chamber technique and chemotaxis or random migration determined with the under-agarose technique (r = -0.38 and 0.12 respectively). Zymosan-activated serum attracted a higher number of leukocytes than did fresh serum, whereas heat-inactivated serum attracted a lower number. This attraction seems to be partly caused by C5a, as a higher C5a-concentration was detected in zymosan-activated serum and in fresh serum after 24 hours in a collection chamber than in heat-inactivated serum.

Adult↗