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Biomedical subjects

A Fuks

Publications and source records attributed to A Fuks.

At least 91 records · Page 5Linked to original sources
PubMed1981

Immunological cross-reactivity between simian virus 40 large T antigen and D2 hybrid T antigen.

A specific antiserum was raised in rabbits against D2 hybrid T antigen that had been purified from HeLa cells infected with the adenovirus/simian virus 40 hybrid, Ad2(+)D2. The specificity of this serum was compared with that of a conventional hamster antiserum against simian virus 40-induced tumors by immunoprecipitation and by a new radioimmune assay that can detect nanogram quantities of D2 hybrid T antigen.

Adenoviruses, Human↗
PubMed1981

Factors affecting the release of proteases from peripheral blood monocytes.

Serum amyloid A (SAA) protein is degraded by serine proteases associated with the external plasma membrane of peripheral blood monocytes. The plasma membrane-associated enzymes are serine proteases of high molecular weight. In addition, cells grown in suspension release enzymatic activity into the surrounding medium. The origin and nature of the released SAA degrading enzymes remains obscure, but they appear to belong to the general class of serine proteases. Conditions of culture were determined which minimized release of the secreted enzymes and will ultimately permit the characterization of the properties of the membrane associated enzymes uncontaminated with the cytoplasmic proteases. It seems likely that there are two compartments of proteases-one surface-associated and one cytoplasmic.

B-Lymphocytes↗
PubMed1978

Possible detection of HLA-DR alloantigenic specificities in man with unabsorbed rabbit antisera.

Thirty-six rabbits were immunized with precipitin lines formed between detergent solubilized membrane fractions from HLA-D-homozygous cells and a rabbit antiserum to human B cells (anti-p 23/30). Thirty-one of the rabbits produced B-cell specific cytotoxic antibodies and twelve of the antibodies were also precipitating in gel diffusion. In cytotoxicity tests six of the antisera showed clear preferential reactivity with the immunizing HLA-D (DR) antigen. Thus immunization of rabbits might prove valuable for the production of HLA-DR typing reagents.

Animals↗
PubMed1977

HLA-D associated alloantisera react with molecules similar to Ia antigens.

Two human alloantisera specific to bone marrow-derived lymphocytes (B cells) were shown to precipitate polypeptide chains of 29,000 and 34,000 daltons from human lymphoblastoid B cell lines. These molecules are similar to murine Ia antigens and are also precipitated by a rabbit B-cell specific heteroantiserum. Since the alloantisera are thought to recognize determinants coded by HLA-D or closely linked loci, these data support the hypothesis that these B-cell specific alloantigens are the human counterpart of the mouse Ia antigens and may be the products of HLA-D.

B-Lymphocytes↗
PubMed1976

Heterogeneity of the protein moiety of carcinoembryonic antigens.

N-terminal amino acid sequences were determined for the protein moiety of carcinoembryonic antigen (CEA) isolated from three colon cancers that had metastasized to the liver. The results showed that the polypeptide portion of CEA preparations isolated from different tumors are not identical. Furthermore, each CEA preparation appeared to be heterogeneous within itself, since multiple amino acid residues were recovered at most of the positions following stepwise cleavage of the protein moiety of CEA molecules by automatic Edman degradation.

Amino Acid Sequence↗