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Biomedical subjects

A Grzybowski

Publications and source records attributed to A Grzybowski.

45 records · Page 3Linked to original sources
PubMedDate not supplied

The ability of granulocytes to absorb latex particles and Staphylococcus aureus in patients hypersensitive to aspirin.

An examination of the ability of granulocytes to absorb latex particles and Staphylococcus aureus was performed in 32 patients with atopic asthma, 27 patients with atopic asthma and hypersensitive to aspirin, and 20 patients hypersensitive to aspirin only. The control group included 20 healthy subjects. The results of our investigation demonstrate a defect of the ability of granulocytes to absorb latex particles and S. aureus in patients with atopic asthma and hypersensitivity to aspirin.

Adolescent↗
PubMedDate not supplied

Granulocyte migration in vivo and in vitro in healthy children of parents with infectious asthma.

Our study was carried out on 28 healthy children aged 6-11 years (mean = 9 years) with at least one parent having infectious asthma, and 13 children aged 7-14 years (mean = 9 years), both of whose parents had infectious asthma. The age of the affected parents was 26-45 years (mean = 36 years). The control group comprised 20 healthy subjects aged 25-37 years (mean = 32 years) and 20 healthy children aged 6-14 years (mean = 9 years) with no family history of atopic diseases. In all subjects, the test of granulocyte migration in vitro was carried out using the method of Clausen, modified by us. The in vivo test was performed according to the Southam method. The results of our investigations demonstrate a defect of granulocyte migration in patients with infectious bronchial asthma. In healthy children, both of whose parents had infection, the migration of the granulocytes was impaired.

Adolescent↗
PubMedDate not supplied

The ability of granulocytes of bone marrow tissue and vascular pools in atopic patients to absorb latex particles and Staphylococcus aureus.

In 68 patients with atopic asthma the ability of the granulocytes from the marrow tissue and vascular pools to engulf neutral latex particles and Staphylococcus aureus bacteria was studied. In patients with atopic asthma at the time of a lull in the disease, an impairment of engulfing of latex particles and S. aureus by the granulocytes in the marrow and vascular pools was demonstrated. The ability of absorbing bacteria and latex by the granulocytes in the tissue pool was similar in the group of patients and in the control group. At the time of asthmatic dyspnoea the ability of engulfing of S. aureus and latex by marrow pool granulocytes was rising steeply, while vascular and tissue pool granulocytes showed an even greater impairment of this ability than at the time of absence of dyspnoea.

Adolescent↗
PubMedDate not supplied

The migration, phagocytosis and reduction ability of granulocytes from skin infiltrations of patients with atopic asthma.

The migration, phagocytosis and reduction ability of granulocytes from the skin of atopic asthmatics (n = 30) and healthy control subjects (n = 20) were evaluated. In all subjects, granulocyte migration was evaluated in vitro using the Clausen test and in vivo using the Matusiewicz and Brzezińska test, and Park's tests of phagocytosis of neutral latex particles and the reduction of nitroblue tetrazolium (NBT) were also performed. It was demonstrated that patients with atopic asthma had a defect in skin granulocyte migration. Our studies also showed that the ability of granulocytes from skin infiltrations to reduce NBT was increased. The ability of granulocytes from skin infiltrations to migrate was inversely proportional to the degree of oxidative burst in patients with atopic asthma.

Adolescent↗
PubMedDate not supplied

Synthesis of hyaluronic acid by human peritoneal mesothelial cells: effect of cytokines and dialysate.

OBJECTIVE: To assess effects of the inflammatory cytokines (IL-1-beta, TNF-alpha, TGF-beta 1) and dialysate effluent on synthesis of hyaluronic acid by human peritoneal mesothelial cells (HMC) in in vitro culture. METHODS: Dialysate effluent was collected after the overnight dwell of Dianeal 1.5% from patients during CAPD training. HMC were obtained from omentum from nonuremic donors or were harvested from the dialysate effluent from CAPD patients. Synthesis of hyaluronic acid was studied on monolayers of HMC, which were deprived of serum 48 hours prior to experiment. Effects of cytokines were tested in a medium with low serum concentration (0.1%) or in medium mixed (1:1 v/v) with the autologous dialysate. Hyaluronic acid level in medium was measured with radioimmunoassay. RESULTS: Cytokines enhanced synthesis of hyaluronic acid by HMC, and the strongest effect was induced by IL-1. Effluent dialysate stimulates synthesis of hyaluronic acid stronger than 10% FCS. Effluent dialysate and IL-1 synergistically enhance synthesis of hyaluronic acid by HMC. CONCLUSION: Effluent dialysate from CAPD patients stimulates production of hyaluronic acid by HMC and acts synergistically with cytokines.

Cells, Cultured↗