PubMed Health⌕ Search

Biomedical subjects

A Holst

Publications and source records attributed to A Holst.

48 records · Page 3Linked to original sources
PubMed1977

[Extracorporeal perfusion with baboon liver in the treatment of hepatic coma (author's transl)].

In 11 patients with hepatic coma (stage IV and V according to Abouna) extracorporeal haemoperfusion using the Scribner shunt (radial or profunda femoris artery) was performed over 12 to 27 hours with 22 baboon and one human livers. Eight patients emerged from coma, six of them showed sufficient regeneration of the diseased liver. Four patients were discharged as cured, one patient died of acute pancreatic necrosis, a further one due to bleeding from an old gastric ulcer. In the 2 remaining patients the coma recurred within 48 hours. Tree patients never came round from coma. After perfusion no antibodies against baboon proteins were demonstrable in the patients. Thus there is very little danger of an anaphylactic reaction when perfusion is repeated. The titre of preformed cytotoxic antibodies against baboon cells in patients' serum rises only after 1-2 weeks and decreases again after 4 weeks. In our experience extracorporeal liver perfusion with baboon or human livers is the most promising method for treatment of hepatic coma.

Adult↗
PubMed1977

[Active enhancement of canine liver allograft by pretreatment with polyspecific spleen alloantigen (author's transl)].

Because it is not possible to use donor specific antigens for the induction of immunological enhancement in cadaveric organtransplantation, attempts were made to use polyspecific antigens in the enhancement of orthotopic canine liver allotransplants. Of 34 mongrel recipients, 17 controls survived for 6.9 +/- 1.5 days. Six recipients (group 3) were given 750 mg/kg polyspecific, semisoluble antigen prepared from 20 spleens (PSEA 20) together with 10 mg/kg prednisolone on days 15, 8 and 1 before transplantation. The mean survival time of this group was 10.1 +/- 2.0 days (P less than 0.01 compared with the control group). Six other recipients (group 4) were treated similiarly, except that the antigen had been prepared from a pool of 70 spleens (PSEA 70). Survival was variable here: 3 survived for more than 3 weeks and the other 3 died on days 1, 7 and 8 post-operatively, with signs of accelerated rejection. Donors and recipients were not identical for LD determinants, but one donor recipient pair with near identity showed a higher degree of enhancement. The recipients displaying accelerated rejection had markedly higher lymphocytotoxic and haemagglutinating antibodies. Animals surviving for longer periods had low antibody titres. In addition, all recipients progressive rejection were found to show inhibition of leucocyte migration. After three antigen doses rosette-forming lymphocytes were present in increased numbers in peripheral blood, and remained unchanged thereafter.

Animals↗
PubMed1977

Detection of serum-blocking factors by inhibition of allorosette formation in rats with long-surviving renal allografts following short-term postoperative ALS treatment.

Thirty-nine (LEW x BN)F1 kidneys were transplanted to LEW rats. Twenty-four untreated recipients survived for a mean time of 16.1 +/- 1.7 days (group 1). Fifteen recipients received 4 ml of antilymphocytic serum per rat (group 3). In the last group 10 recipients survived for more than 4 months. The spleen cells of these permanently surviving 10 rats were obtained by splenectomy and used in a graft-versus-host assay, and this assay showed that the reactivity of these cells was normal. Following splenectomy the animals were given an (LEW x BN)F1 skin allograft, followed 18 days by a second. After another 18 days (LEW x Buf)F1 "third party" skin allografts were transplanted to the same animals. Animals of group 2 rejected their first grafts with a mean survival time of 12.2 +/- 1.2 days, whereas the second grafts were rejected normally as were the third party grafts. Attempts were made to detect lymphocytotoxic antibodies and haemagglutinins before and after the transplantation of skin grafts and none could be found up to day 53. The sera of group 2 inhibited allorosette formation by 38%. This serum-blocking factor was donor specific. It is probable that the survival of the kidney transplants following antilymphocytic serum treatment was brought about by the development of blocking antibodies.

Animals↗
PubMed1977

Proper use of skin tests with food extracts in diagnosis of hypersensitivity to food in children.

This study was undertaken to determine the proper use of skin tests with food extracts in diagnosis of hypersensitivity to food in children. Cutaneous reactions evoked by graded amounts of food extracts were compared with results of double-blind food challenge and in vitro release of histamine from leucocytes. A 3 mm or greater weal reaction in skin tests by puncture technique using food extracts of 1:20 w/v concentration was found to indicate the degree of hypersensitivity likely to be associated with clinically significant hypersensitivity reactions to food. Proper use of this simple technique will facilitate accurate diagnosis of food hypersensitivity in children by identifying the group among whom all positive reactions to food challenges will be found. Nevertheless, double-blind food challenge is essential to establish a diagnosis of symptomatic hypersensitivity to food.

Adolescent↗
PubMed1976

[Treatment of comatose patients by extracorporeal hemoperfusion with activated charcoal].

Hemoperfusion with activated charcoal is indicated in intoxications and in hepatic comma because the charcoal absorbs toxic substances. Good results have been obtained so far with activated charcoal liver perfusion in barbiturate intoxications, but less satisfactory results in hepatic comma. The indication for perfusion should also be made in clinically hopeless cases. The principal complications with activated charcoal perfusion are a decrease in thrombocytes and microemboli of charchoal particles. These complications can be avoided by coating the charcoal particles. A flow-rate of 200-300 ml/min is best in humans because this flow-rate ist guaranteed by an AV shunt in the forearm.

Capsules↗
PubMed1976

[Immune status of indefinitely surviving rat renal allograft recipients after short course of ALS-treatment Detection of a serum blocking factor by allorosette formation inhibition test (author's transl)].

53 LBNF1 kidneys were grafted into L-rats. 24 untreated recipients served as control and survived 16.1 +/- 1.7 days. 14 recipients were treated with 4 ml alloantiserum (ADS) each, and the other 15 animals with 4 ml ALS each. 9 and 10 of these 14 and 15 animals survived for more than 4 months. We performed GvH-reaction with spleen cells of these 19 animals. It showed normal cellular immune response. After splenectomy first donor specific skin was transplanted, 18 days later second skin of same origin (LBNF1) and 18' days after third party skin (LBufF1). The first grafts survived 13.1 +/- 1.9 days in the recipients of ADS treatment and 12.2 +/- 1.2 days in the recipients of ALS. Second skin grafts were rejected delayed in the former recipients as the first grafts, in the later more accelerated than the first grafts. The third grafts survived as the controls. Before and after skin grafting we could not detect lymphocytotoxin and hemagglutinin. The sera of animals with ADS therapy inhibited the spontaneous allorosette formation to 46.0 +/- 4.4% and of ALS therapy 37.7 +/- 9.2%. Our results suggest that after ALS treatment blocking antibodies were produced in the recipients and they cause the enhancement of kidney allograft.

Animals↗
PubMed1976

[Active enhancement of canine kidney allografts (author's transl)].

In 32 beagles heterotopic renal allotransplantations and bilateral nephrectomies were carried out. Control animals (5 dogs) survived 9.4 +/- 1.6 days. 22 recipients were pretreated with donor-specific semisoluble spleen antigen; 5 recipients pretreated with the antigen alone (780 mg/kg body weight) survived 16.6 +/- 2.3 days. A pretreatment with antigen combined with 5 mg/kg body weight Prednisolone resulted in no significant prolongation of survival time (5 animals), but 5 dogs survived significantly longer after pretreatment with the antigen and 50 mg/kg body weight Prednisolone (27.4 +/- 2,7 days). In 5 recipients after preoperative application of 3 doses Prednisolone alone (50 mg/kg body weight each) no prolongation of survival time could be observed (10.2 +/- 0.7 days). We found no correlation between lymphocytotoxic antibody titre and survival time.

Animals↗
PubMed1976

[Demonstration of blocking factors in permanently surviving rat-kidney allograft recipients after short postoperative treatment with alloantiserums].

GvHR tests were performed on spleen cells of 9 recipients surviving rat renal allografts for more than 120 days. The recipients had undergone a short term ALS-treatment. The GvHR tests showed normal cell-mediated immunity. After splenectomy and again 18 days later LBNF1 skin was grafted to the renal recipients. After a further period of 18 days LBufF1 skin was grafted. After some delay the specific grafts were rejected. The unspecific skin was rejected in a normal way. Before and after skin grafting the authors could not find the lymphocytotoxin and hemagglutinin. Instead a blocking factor which inhibited the spontaneous allorosette formation was detected in the sera of recipients. The authors compared the results with passive enhanced renal recipients. The two groups did not differ significantly from each other.

Animals↗
PubMed1975

[The effect of donorspecific antigen type for active enhancement of renal-allografts in the histoincompatible inbred rats (author's transl)].

49 kidneys of male LBNF1-rats were transplanted into male Lewis-rats. BN-rats were used as a source of antigen. Control animals survived 16.1 +/- 1.8 days. 5 recipients were preteated with donorspecific living cells, 7 with semisoluble and 5 with low dosage of soluble antigen. The living cell-pretreatment showed the best effect for the active enhancement, the soluble antigen the least. The pretreatment with 6 mg protein/kg bw. caused the sensibilization of recipients. At the time of transplantation lymphcytotoxic antibody titers were detected in all recipients with pretreatment of living cells, but no titer in other groups. Renal allografts with elevated postopertive titer were rejected rapidly.

Animals↗
PubMedDate not supplied

[Complications following autologous bone transplantation at the site of removal].

Between April 1978 and December 1983, 206 operations to obtain autologous bone were performed in the Orthopedic Department of Barmbek General Hospital. In 1985, 125 patients were analyzed by means of a detailed questionnaire and a clinical examination. A total of 55.2% of the 125 patients were free of complaints following removal of bone. In 37.6% there were slight to moderate, and in 7.2% severe to extremely severe complications. The most common type of complaint was persistent postoperative pain (40%). Neural damage, i.e., hypesthesia and dysesthesia, was observed in 21.6% of the cases. An increased postoperative tendency to swelling was seen in 12.8% of the 125 patients. There was also one case of fracture of the shaft of the tibia in the area where bone had been removed. Other complications were rare. All in all, the postoperative complications following removal of a chip from the shin were the most serious. Complaints due to removal from the posterior iliac crest were considerably less severe. The least problematic courses were those after removal from the anterior iliac crest. Removal of coritcospongious chips from the tibia should be avoided if at all possible. The removal site of choice is the anterior iliac crest, unless the posterior crest has to be preferred because of the surgical technique employed.

Bone Transplantation↗
PubMedDate not supplied

Treatment of extrahepatic occlusive jaundice with activated charcoal hemoperfusion in dogs.

To find the feasibility of treatment for congenital bile duct atresia, we studied the usefullness of extracorporeal hemoperfusion over activated charcoal in canine obstructive jaundice. One, three and five weeks after ligation and disection of common bile duct in 5 dogs we performed the hemoperfusion over activated charcoal extracorporeally (group 3). In this animals we examined hematological and blood coagulation studies, serum electrolyte levels, kidney function tests and liver chemistries. As control in 5 animals we carried out after sham operation the perfusion without common bile duct ligation (group 2) and in 5 animals only common bile duct ligation without perfusion (group 1). In the liver chemistries we found 2 weeks after 2nd and 3rd perfusion (5 and 7 weeks after bile duct ligation) lower levels of serum bilirubin, GOT, GPT and SDH in treated group than in non-treated jaundiced animals. It suggest the effectiveness of hemoperfusion with activated charcoal in the treatment of occlusive jaundice. There were no alteration in the hematological studies, serum electrolyte levels and kidney function tests. PT and PTT was prolonged in the jaundiced animals there were no significant differences with and without hemoperfusion.

Animals↗