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A I Ageenko

Publications and source records attributed to A I Ageenko.

68 records · Page 4Linked to original sources
PubMed1969

[Cancer virus].

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Oncogenic Viruses↗
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[Splenocyte autoreactivity of mice of sensitive lines during the latent period of carcinogenesis induced by virus SA7(C8)].

The method of adsorption on a monolayer of embryonal fibroblasts of 51Cr-labelled spleen cells from CBA and C57B1/6 mice infected at birth with SA7(C8) virus was used to show that the cells immune to the antigens of mouse embryo fibroblasts accumulated in the spleen of CBA mice highly susceptible to oncogenesis induced by SA7(C8) virus. In the controls, splenocytes of intact CBA mice were adsorbed on embryonal fibroblast monolayers (the percentage of adsorption 9.8 and 4.2, respectively; P less than 0.05). In the spleen of C57B1/6 mice insusceptible to oncogenesis induced by SA7(C8) virus there was no accumulation of cell antibody to the antigens of embryonal fibroblasts (P greater than 0.1). The detected antibody caused no cytotoxic effect on embryo fibroblasts.

Animals↗
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[Isoenzymes of lactate and malate dehydrogenases in nuclei of rat embryo fibroblasts infected with oncogenic adenovirus type 12].

The isoenzymatic spectrum of lactatedehydrogenase (LDG; L-lactate: NAD-oxireductase; EC 1.1.1.27) and malatedehydrogenase (MDG; 1-malate: NAD-oxireductase; EC 1.1.1.37) in nuclei of rat embryo fibroblast cells infected with adenovirus type 12 was studied at 3, 5, 8, 18 and 24 days of cultivation. The nuclei were isolated according to the method of Showo et al. modified by Zbarsky and Georgiev. Proteins were fractionated by disc electrophoresis in polyacrylamide gel. In the course of oncovirus-cell interaction three LDG isoenzymes were detected. Changes in the isoenzymatic spectrum of LDG were manifested in increased activity of LDG1 at 5 days after virus penetration into the cell. Partial morphological transformation at 18 days after infection was accompanied by increased activity of LDG1 and LDG2. The morphologically transformed culture was characterised by reduced LDG1 activity. Changes in the spectrum of MDG isoenzymes were detected at 3 days after addition of A-12 virus into the culture. At 24 days postinfection activity of MDG3 was definitely changed in nuclei of REF cells infected with A-12 virus. The revealed disorders in regulation of synthesis of individual isoenzymes are probably due to the epigenomic effect of oncovirus and may be used as indications of tissue culture malignization.

Adenoviridae↗
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[Antitumor effect of a tumor-cell neuraminidase vaccine].

The effect of neuraminidase vaccine of CBA mouse sarcoma cells caused by simian adenovirus SA7 (C8) on growth parameters of transplanted sarcoma in a syngeneic system was studied. Inoculation of the vaccine was found to inhibit tumor growth. This effect was more marked in a group of animals given tumor cells after preliminary vaccination. There was no correlation between tumor growth parameters and cytotoxic activity of spleen cells assesses in the cytotoxicity test by 51Cr release in the groups of vaccinated and control animals. It is concluded that the treatment of tumor cells with neuraminidase increases their immunogenicity. The cytotoxic activity of spleen cells in vaccinated animals appears earlier and persists longer.

Adenoviruses, Simian↗