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Biomedical subjects

A I Ageenko

Publications and source records attributed to A I Ageenko.

At least 55 records · Page 3Linked to original sources
PubMed1976

[Activity of hexokinase isoenzymes in the nuclei of cell cultures infected with oncogenic type-12 adenovirus].

The isoenzymic spectrum of a glycolytic enzyme-hexokinase (HK. 2,7.1.1) in the globuline fraction of cell nuclei of rat embryo fibroblasts (REF), infected with human adenovirus of type 12, was studied at 3, 5, 8, 18, 24 days of cultivation. Control studies revealed 3 hexokinase isoenzymes, the third one showing the highest activity and electromobility. In cultures under experiment the first enzyme was found to be lost at 5, 8, 18 days of infection and its activity to be enhanced on the 24th day. The activity of enzymes II and III increased yet on the 3d day of the culture infection. These alterations were especially pronounced to the start of morphological transformation (18th day) and at the 24th day, when the culture was entirely transformed.

Adenoviridae↗
PubMed1976

[Mitotic cycle in cell cultures infected with oncogenic type-12 adenoviruses].

Oncogenic adenovirus of type 12 in the culture of rat embryo fibroblasts, in which it induces an abortive infection, and in HEp-2 culture, whereas it shows reproduction and causes lytic infection, in the early stationary phase causes shortening of the mitotic cycle and its separate periods. Reduction of the mitotic cycle duration occurs on account of shortening of the presynthetic stage and DNA synthesis stage. This property of the virus is in agreement with its capacity to stimulate proliferation of infected cells and is likely to be one of the necessary conditions for development of malignant transformation.

Adenoviridae↗
PubMed1976

[Hexokinase activity in the soluble fraction of the nucleus and cytoplasm in cell cultures infected with human adenoviruses types 6 and 12].

A study was made of the activity of hexokinase in the soluble fraction of the nucleus and cytoplasm of fibroblasts of rat embryos (primary cultures) during contact with infectious (A6) and oncogenic (A12) human adenoviruses. A high activity of the enzyme was seen in the cytoplasmic fraction in comparison with the nuclear one both in the infectious adrenovirus of type 6 this value differed from the normal and in the experimental samples. With the action of the normal but insignificantly. Marked changes in the enzyme activity were observed in case of cell contact with the oncovirus. They occurred both in the nucleus and in the cytoplasm the first days of the virus inculcation, increasing gradually with the lapse of tiay; these disturbances were much greatert in the cytoplasm than in the nucleus. It is supposed that the changes revealed possibly associated with the impossibility to regulate the cell proliferation process.

Adenoviridae↗
PubMed1975

[Detection of adenovirus type-12 in sarcomas induced by this virus in hamster].

Cells of transplantable sarcomas primarily induced in hamsters with adenovirus, type 12, produced persistantly this virus in an infective form. In sera of sarcomabearing hamsters virus-neutrolizing antibodies and antibodies to T-antigen of tumors were revealed. The presence of T-antigen in cells of all types of sarcoma A-12 was demonstrated by means of immunofluorescence, as well as the absence of a specific luminescence of surface membranes of A-12 MML sarcoma cells, obtained from pulmonary micrometastases.

Adenoviridae↗
PubMed1975

[Ultramorphometry of cells in a hamster embryo fibroblast culture following transformation by Rous sarcoma virus].

Changes in mitochondria, ergastoplasma and the polyribosomal apparatus of FHE (fibroblasts of hamster embryo) cell culture were studied in the kinetics of their malignification with Rous sarcoma virus. Considerable alterations were revealed in the organization of protein-synthetising cytoplasmic organells, manifested in the pronounced enhancement of nonspecific protein synthesis and reduction of ergastoplasma. This process starts immediately after the infection and shows a character approximating the exponents. On the part of the mitochondrial apparatus no differences between the experiment and control were revealed.

Animals↗