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A I Gusev

Publications and source records attributed to A I Gusev.

51 records · Page 3Linked to original sources

Interfacing matrix-assisted laser desorption/ionization mass spectrometry with column and planar separations.

This paper presents a critical review of off-line and on-line coupling of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry to liquid column separations (e.g. HPLC, GPC and CE) and planar separations (e.g. PAGE and TLC). Off-line MALDI analysis of fractions collected from HPLC, GPC and CE or spots scraped and extracted from TLC and PAGE has already become a routine practice for many laboratories. MALDI has also been used to obtain mass spectra directly from TLC plates and PAGE. The direct analysis methods range from dot-blotted samples to two-dimensional scanning of the entire gels/plates. Various combinations of on-line coupling of MALDI with column separations are also reviewed. The review discusses the strengths and limitations associated with different off-line and on-line coupling approaches.

Animals↗

[Analytical characteristics of commercial IRMA-AFP kit for determination of human alpha-fetoprotein].

Commercial polyclonal IRMA-AFP kit is manufactured by Radiopreparat in Tashkent since 1986. The kit has a range of 0 to 320 ng/ml. This report includes the data on AFP measurements in the sera of cancer patients, particularly at the lower part of the calibration curve. Within run precision studies yielded a CV of 7.7% at the 4.3-54.0 ng/ml interval. Between run precision was 8.6% at the 3.3-56.0 ng/ml interval. The recovery of AFP in the presence of matrix effects of 9 sera was 98% for mixtures at the 9.1-60.8 ng/ml range. The assay sensitivity was 0.6 ng/ml. In donor blood samples the AFP levels did not exceed 4 ng/ml.

Blood Donors↗