PubMed Health⌕ Search

Biomedical subjects

A J Brookes

Publications and source records attributed to A J Brookes.

6 recordsLinked to original sources
PubMed1991

Coincident sequence cloning.

We describe a novel method, Coincident Sequence Cloning (CSC), which permits the selective recovery of common sequences shared between two complex and partially coincident DNA mixtures. We evaluate this method by integrating human DNA with DNA from a mouse-human somatic cell hybrid, and we recover exclusively human DNA products which are all represented in the hybrid genome. CSC strategies should be useful in addressing many highly complex problems in genome analysis.

Animals↗
PubMed1989

Evaluation of the use of S1 nuclease to detect small length variations in genomic DNA.

A method which utilises S1 nuclease to detect small length variations in cloned and genomic DNA has been evaluated. The methodology of this technique is simple and robust, permitting the rapid analysis of 10(4) base pairs. By employing defined sequence variants, this method is shown to have a sensitivity which should enable the detection of length variations of only a few base pairs in heterozygous individuals.

Base Composition↗
PubMedDate not supplied

Coincident sequence cloning: a new approach to genome analysis.

Many analytical molecular genetic techniques developed over the past decade have evolved primarily to tackle the problem of targeting or isolating sequences of interest in complex mixtures. A new approach to this problem, Coincident Sequence Cloning (CSC), involves integrating a pair of DNA mixtures in such a way as to isolate any shared sequence components.

Cloning, Molecular↗