Coincident sequence cloning.

A J Brookes; D J Porteous

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PubMed · 2041738

Coincident sequence cloning.

A J Brookes·D J Porteous

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Abstract

We describe a novel method, Coincident Sequence Cloning (CSC), which permits the selective recovery of common sequences shared between two complex and partially coincident DNA mixtures. We evaluate this method by integrating human DNA with DNA from a mouse-human somatic cell hybrid, and we recover exclusively human DNA products which are all represented in the hybrid genome. CSC strategies should be useful in addressing many highly complex problems in genome analysis.

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Animals Base Sequence Blotting, Southern Cloning, Molecular DNA Evaluation Studies as Topic Humans Hybrid Cells Mice Molecular Sequence Data Polymerase Chain Reaction Journal Article

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A J Brookes, D J Porteous. 1991-05-25. Coincident sequence cloning.. https://pubmed.ncbi.nlm.nih.gov/2041738/

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