PubMed Health⌕ Search

Biomedical subjects

A J Pesce

Publications and source records attributed to A J Pesce.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

Application of ELISA and ELADA assay (double substrate immunoassay) to red blood cell antigens.

A double substrate immunoassay has been developed that allows simultaneous quantitation and localization of red blood cell surface antigens. The assay can be used to quantitate surface antigens, or the titer of antisera specific to cell-surface antigens, on the red blood cell. The assay has been adapted to the study of both D antigen and erythrocyte membrane (EM) antigen, which were found to be uniformly dispersed over the surface of the cell. The number of EM antigens per cell appeared to be approximately twenty times that of the D antigen. For typing antisera, this assay makes it feasible to express antibodies in absolute units (microgram antibody) rather than conventional dilution titers, and thus offers a simpler means of rapidly comparing antisera.

Blood Group Antigens↗
PubMedDate not supplied

Amoxapine in human overdose.

Amoxapine, a tricyclic antidepressant, is metabolized to 8-hydroxyamoxapine and 7-hydroxyamoxapine. There are few reports on the metabolism of this drug and correlation of clinical symptoms in overdose patients. Five such patients admitted to the Emergency Unit of the University of Cincinnati Hospital were studied. Clinically, all had seizures and evidence of altered cardiac function. The amounts of the parent drug and the 7- and 8-hydroxy metabolites were measured and, in all cases, the parent and 8-hydroxy metabolite were present in both urine and serum. In contrast, the 7-hydroxyamoxapine was found in trace amounts in the serum of only two patients, but in the urine of all the patients observed. These observations were confirmed by gas chromatographic/mass spectroscopic analysis. The pattern of metabolism was analogous to that found in patients on maintenance doses of the drug. In two overdose patients, it was possible to monitor the levels as a function of time. The elimination curve of parent and metabolite was first order with a half-life of 8.5 to 15.0 and 48 hr, respectively.

Adult↗
PubMedDate not supplied

Determination of polysorbate in ascites fluid from a premature infant.

A method is described for the determination of poly(oxy-1,2-ethanediyl)oligomers in body fluids using ammonium cobaltothiocyanate complexation in conjunction with high pressure liquid chromatography and visible spectrophotometry. Analysis of peritoneal fluid from a baby given E-ferol, a vitamin E supplement, revealed levels as high as 100 micrograms/mL polysorbate.

Ascitic Fluid↗
PubMedDate not supplied

The medical heritage concept: a model for assuring comparable laboratory results in long-term longitudinal studies.

The success of a three or four decade health monitoring program depends upon the constancy and stability of the laboratory measurement data. This paper describes a prospective model which has been implemented as part of a 30-year health care program developed for the benefit of a particular group of residents who have lived for more than two years within five miles of an industrial plant which processed uranium metal and contaminated the surrounding area. Effective long-term laboratory monitoring (20 to 40 years) requires (1) a stable analytical base to establish comparability of all data collected, (2) innovative computer based record keeping, and (3) two selected reference populations which reflect method bias and widespread population change bias. To meet this need for comparability, the long-term Medical Heritage comparability concept was developed. This is an approach to the determination, storage, and retrieval of the laboratory data obtained on each specific participant in such a manner that all of that participant's data are internally comparable and continually traceable to definitive and/or reference methods developed by the National Institute for Standards and Technology and the Centers for Disease Control and the National Committee for Clinical Laboratory Standards. Systematic long-term documentation differentiates the Medical Heritage 30 to 40 year concept from the current short-term two- to three-year data continuity systems. As a model for long-term medical monitoring, the Medical Heritage comparability concept gives validity to individual patient care monitoring decisions.

Adult↗