Biomedical subjects

A Janik

Publications and source records attributed to A Janik.

At least 19 recordsLinked to original sources

PubMed1992

[Effect of cadmium on certain factors of lipid metabolism in the aorta and myocardium of rats].

The goal of the research was to examine the effects of cadmium chloride on the levels of selected components of the lipid metabolism in the homogenates of the aorta and the cardiac muscle. Our experiments were carried out on white rats of the Wistar breed subdivided into three experimental groups. Group I were the control animals. The rats of Group II and Group III received the doses of per os, water solution of the cadmium chloride, of 0.88 mg CdCl2 x 2.5H2O per kg of the body weight and 8.8 mg CdCl2 x 2.5H2O per kg of the body weight, respectively, 3 times a week for 3 months. The concentration of total cholesterol was determined in the aorta homogenates and the concentrations of free fatty acids, triglycerides, phospholipids and total cholesterol were determined in the cardiac muscle homogenates. On the basis of our results we found the increase of the total cholesterol concentrations in the aorta homogenates. We also observed the tendency to increase the total cholesterol level in the cardiac muscle homogenates. Such results indicate that atherosclerotic changes of vessels are possible in conclusion of a chronic cadmium poisoning. No changes in the concentrations of the lipid components which are energetically important for the heart were found. Besides, the decrease of the phospholipid concentration resulting from the cadmium chloride poisoning was observed in the cardiac muscle homogenates. That may indicate a change of the activities of the respective enzymatic systems in the cardiac muscle under the influence of cadmium which also causes damages of biological membranes.

Animals↗

PubMed1991

Cosegregation of two allotypes G9 and G16 with the alpha-protease inhibitor variants in pigs.

The association between genes encoding for two serum protein antigens (G9 and G16) and alpha-protease inhibitors was analysed in pigs. A recombination frequency between PI and locus controlling G9 and G16 allotypes varied from 0 to 4.8 cM. Data from appropriate matings are consistent with a gene order of PI1-PO1A-PO1B-PI2-(G9, G16).

Alleles↗

PubMed1991

[The toxic effect of methylmercuric chloride on the organism in light of research on the hematopoietic system and metabolism of carbohydrates and lipids in heart and liver].

The purpose of our experiments was to demonstrate possible changes in the activities of the hematopoietic system and the metabolism of the cardiac muscle and liver in the condition of the subacute poisoning with the methylmercuric acid. The tests were performed on 310 rats. The animals were administered the methylmercuric chloride per os in three different doses during three weeks. The activity of the hematopoietic system was analysed on the basis of selected factors concerning the erythrocytic system (the number of reticulocytes and erythrocytes, hematocrit, hemoglobin concentration and the osmotic resistance of erythrocytes), the leukocytic system (number, percentage composition and the osmotic resistance of leukocytes), and the thrombocytes. The alterations in the cardiac muscle and the liver were analysed on the basis of selected elements of the carbohydrate and lipid metabolisms. The indicators of the carbohydrate metabolism were glycogen, pyruvic, lactic, and citric acids. For the lipid metabolism we determined the concentration of free fatty acids, triglycerides, cholesterol and phospholipids. A tendency to increase the minimum osmotic resistance of erythrocytes appeared under the influence of the methylmercuric chloride, probably as a result of the binding between the absorbed methylmercury with lipids and with the proteins of the erythrocyte cell membranes. As to the percentage composition of leukocytes, we observed the reduction of the number of eosinophils in the peripheral blood. The rats poisoned with the methylmercuric chloride reacted to the administered foreign toxic substance with the excitation of their reticuloendothelial systems which was demonstrated by a very clear increase of the reticular cells number. We found a reduction of the content of the basic energy substrate in the cardiac muscle, i.e. the free fatty acids, with the parallel increase of triglyceride concentration. The reductions of the glycogen and lactic acid concentrations were observed primarily in the carbohydrate metabolism of the heart. Similar changes in the methylmercuric chloride poisoning were found in the liver. Also here the methylmercuric chloride caused the reduction of the glycogen and lactic acid concentrations as well as the reduction of the number of free fatty acids and the increase of the triglyceride concentration. The results of the above experiments show that the methylmercuric chloride did not distort considerably the activities of the hematopoietic system and the observed changes were not extensive. In both tested tissues we identified biochemical changes that can indicate the changes in the carbohydrate and lipid metabolism in both organs.(ABSTRACT TRUNCATED AT 400 WORDS)

Animals↗

PubMed1979

Esterolytic activity of blood serum in infants with hypertrophic pyloric stenosis.

Esterolytic activity of blood serum was determined in infants with hypertrophic pyloric stenosis and in healthy control children. The substrates hydrolyzed mainly by arylesterase (EC. 3.1.1.2), i.e. p-nitrophenyl esters of acetic, propionic and butyric acid were used. It was found that in infants with hypertrophic pyloric stenosis the rate of p-nitrophenyl propionate hydrolysis was significantly higher than that of the acetate or butyrate. In control group children all three substrates were hydrolysed at similar rates, and these were significantly lower than in infants with hypertrophic pyloric stenosis.

Carboxylic Ester Hydrolases↗

PubMed1979

Protein-cationic detergent interaction. Interaction of bovine serum albumin and other proteins with alkylpyridinium bromides studied by viscosity, gel filtration and spin-label methods.

Viscosity, gel filtration and spin-labelling methods have been used to study the influence of alkylpyridinium bromides on the conformation of bovine serum albumin and other proteins. Cationic detergents cause partial unfolding of the native protein molecules. The magnitude of these changes increases with increasing length of the detergent hydrocarbon chain. When cationic detergents are added to reduced and carboxymethylated bovine serum albumin the observed changes are opposite to those found in native protein.

Animals↗

PubMed1979