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Biomedical subjects

A L Liozner

Publications and source records attributed to A L Liozner.

At least 19 recordsLinked to original sources
PubMed1988

[Rapid immunoenzyme diagnosis of influenza in patients with various allergoses and bronchial asthma].

In an investigation carried out in the allergological clinic of the Tbilisi State Medical Institute in the period between two outbreaks of influenza, the presence of influenza antigen was determined in nasal washings taken from 127 patients with different allergoses and bronchial asthma by means of the enzyme immuno-assay with the use of the type-specific virion antigen of M1-protein. This method was found to be highly sensitive and to have some advantages over traditional methods used for the diagnosis of influenza. In patients with preasthma and different forms of bronchial asthma elevated susceptibility to influenza infection and its unfavorable influence on the clinical course of these pathological conditions were established.

Asthma↗
PubMed1988

[Immunobiological aspects of AIDS].

The growing pandemia of AIDS, which resulted in about 40,000 AIDS patients and about 3 million infected persons by the end of 1986, demands for the urgent creation of methods for diagnosis, prevention and treatment of the disease. The present paper analyzes the main aspects of AIDS immunobiology, i. e. the molecular genetics of the virus, production of the viral components, construction of kits for immunoenzyme detection of antibodies to the virus, development of confirmatory immunoenzyme assays. The authors discuss the prospects of creating the synthetic antigens of the AIDS virus or producing them on the basis of genetic engineering both for the diagnosis and prophylaxis of the infection.

Acquired Immunodeficiency Syndrome↗
PubMed1986

[Immunoenzyme determination of antibodies to the surface of islands of Langerhans in autoimmune destruction of the insular apparatus].

Enzyme-linked immunosorbent assay (ELISA) was applied for detection of human islet cell surface antibodies (ICSA) to rat islet target cells. In 23 healthy controls without hereditary diabetes the findings were on the upper normal limit (mean value of optical density + 3 SEM). The results above the limit were considered positive. 11 out of 18 insulin-dependent (Type I) diabetics with the disease duration less than 5 years were ICSA-positive. All 9 patients with insulin-independent (Type 2) diabetes were ICSA-negative. 3 out of 18 healthy subjects (siblings and children of probands with type II diabetes) were strongly ICSA-positive, although all the members of this risk group had unimpaired oral glucose tolerance test. Thus, ELISA screening of ICSA may be useful for discriminating patients with different types of diabetes and revealing nonaffected individuals at high risk according to their beta-cell integrity.

Animals↗
PubMed1986

[Stimulation of the immune response to immobilized antigen in an in vitro system with B-activin].

The influence of B-activin, the preparation of immunomodulating myelopeptides, on the level of antibody formation after the primary immunization of mouse splenocyte cultures with immobilized antigens has been studied. The treatment of the cells with B-activin on the third day of their cultivation in the presence of peroxidase immobilized on polystyrene or protein M1 of influenza virus has been found to increase antigen-specific antibody formation by several times, while having practically no effect on the total level of IgG secretion. The stable level of the stimulation of antibody formation and the possibility of its quantitative evaluation in the enzyme-linked immunosorbent assay makes this immune response inducing system a convenient model for testing the biological activity of myelopeptides and other immunostimulators.

Activins↗
PubMed1985

[Analysis of the antigenic and immunogenic properties of protein M of the influenza virus by an immunoenzyme method].

A test system permitting the detection of influenza virus protein M at a concentration of 0.1-0.5 ng/ml in ELISA has been developed. The use of this system made it possible to detect influenza viruses A and B directly in crude virus-containing material and clinical samples obtained from influenza patients. During the outbreak of influenza in the spring of 1983 ELISA was successfully used for the rapid diagnosis of influenza, and some of its advantages in comparison with the conventional immunofluorescence test were thus demonstrated. To overcome difficulties arising from the low immunogenic potency of protein M, in the process of obtaining diagnostic sera and ascitic fluids the animals were immunized with the conjugate of protein M and polyelectrolite, which ensured considerable activation of humoral immune response.

Animals↗