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Biomedical subjects

A L Liozner

Publications and source records attributed to A L Liozner.

At least 37 records · Page 2Linked to original sources
PubMedDate not supplied

[Immunological characteristics of LPV oncornavirus produced by T-9 human cell line].

The results of immunological study of oncornavirus (LPV strain) produced by a continuous human T-9 cell line are presented. The method of immunodiffusion in agar revealed the following constituent antigens in the virus: (a) those identical to internal and external antigens of Mason-Pfizer oncornaviruses particles; (b) those specific only for LPV virus and not common in other known oncornaviruses of mammals. These antigens were common in LPV virus from T-9 culture and from xenogeneic T-BHK culture in which Mason-Pfizer virus antigens were absent completely; and (c) interspecific antigen of oncornavirus C type present in minimal amounts. Localization of the above antigens in virus particles of LPV oncornavirus was confirmed by the electron immunoenzyme method. Upon separation of virus polypeptides in polyacrylamide gel it was found that the antigens cross-reacting with Mason-Pfizer virus as well as LPV specific antigens were localized both in the main inner virus protein and in the surface glycoprotein.

Antigens, Viral↗
PubMedDate not supplied

[A standard panel of human immunodeficiency virus-positive and -negative blood sera for assessing the sensitivity and specificity of diagnostic immunoenzyme test systems].

The materials on the development of a reference panel of HIV-positive and negative blood sera required for quality control of diagnostic enzyme-immunoassay systems are summarized. Ninety sera (50 HIV-antibody positive and 40 negative) were examined in 2 test systems of national and 3 test systems of foreign companies as well as by immune blotting and immunofluorescence tests. Each serum was characterized by optic density values and antibody titer (for positive sera). The conditions of storage and transportation of serum panels as well as those for control tests were determined. The license and instructions for use for the serum panel have been approved. The serum panel is used in 9 institutions engaged in working out and manufacture of diagnostic test systems.

AIDS Serodiagnosis↗
PubMedDate not supplied

[Islet cell surface autoantibodies: their immunoenzyme analysis using rat target cells].

A hard phase immunoenzymatic system for primary screening and subsequent quantitative determination of autoimmunobodies to the surface of islet cells (ASIC) was worked out using rat target cells. ASIC organ-specificity was confirmed by the absence of crossreactions with rat hepatocytes and splenocytes. The determination of the upper normal bound with 99% confidence interval combined with a high sensitivity of the method allowed an objective and significant assertainment of ASIC-positive sera even with a low level of antibodies. ASIC quantitative characteristic is their titer determined with the help of the intersection of a titration curve with the upper normal bound. ASIC were detected approximately in half of the patients with insulin-dependent diabetes of a duration of maximum 5 years and were not detected in the patients with insulin-dependent diabetes compensated with diet and sugar lowering drugs using the above method.

Animals↗
PubMedDate not supplied

[Antigenic properties of the M1 proteins of influenza type B viruses and the detection of a common antigenic determinant of the M1 proteins of type A and B viruses].

Antigenic properties of M1 proteins of influenza B viruses were studied by indirect EIA using monospecific antisera to M1 protein obtained by immunization of rabbits and mice. Antisera with highest titres were obtained by immunization of mice with polyelectrolyte-conjugated M1 protein. Antigenic heterogeneity of M1 proteins of influenza B viruses was demonstrated. M1 proteins of B/Lee/40 and B/USSR/03/84 viruses reacted with B/Hong Kong/72 antiserum to one-fourth of the homologous titre. Cross-experiments using M1 proteins recovered from influenza type A and B viruses and antisera to them revealed heterologous reactions suggesting a common antigenic determinant in A and B M1 proteins. Treatment of the antisera with M1 protein of type A resulted in a decline of antibody titres to M1 proteins of type B. The two available monoclones to M1 protein of type A reacted only with M1 A but not with M1 B.

Adsorption↗
PubMedDate not supplied

[ELISA immunoenzyme detection of the antigens of interferon and protein contaminants].

The possibility of specific detection of interferon antigens by ELISA was demonstrated on a model of partially purified and ultrafiltration-concentrated preparations of mouse fibroblast interferon. ELISA allows differentiation of specific interferon antigens and the main protein contaminants of its preparations: bovine serum albumin and virus-inducer proteins.

Animals↗
PubMedDate not supplied

[Rapid diagnosis of influenza by detecting viral M protein in an immunoenzyme test].

Previously developed ELISA test system with sensitivity about 0.5 ng/ml of virus protein M was used for influenza virus detection in nasopharyngeal washings of patients with acute respiratory virus diseases (ARVD). Altogether 184 specimens from patients with ARVD and 31 from patients with other infections were examined in the pre-epidemic and epidemic periods of 1983. In parallel, virus antigens were detected by direct immunofluorescence test (IF). The total frequency of viral antigen detection by ELISA and IF coincided (63% of the specimens examined). With the specimens collected early in the disease (2-3 days) the rate of virus findings by ELISA rose to 82%. Direct detection of viral antigen in nasopharyngeal washings by means of an objective immunochemical method seems to be promising for a large-scale rapid diagnosis of influenza.

Antibodies, Viral↗