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Biomedical subjects

A L Muggleton-Harris

Publications and source records attributed to A L Muggleton-Harris.

45 records · Page 3Linked to original sources
PubMed1976

The nature and distribution of serologically detectable alloantigens on the preimplantation mouse embryo.

The nature and distribution of surface alloantigens on preimplantation mouse embryos has been examined by immunofluorescence. Non-H-2 alloantigens were detected at all stages examined, from the 2-cell to the 4 1/2-day blastocyst. Cleaving blastomeres, inner cell mass cells and cells of the primary trophectoderm were all positive. In F1 embryos maternal non-H-2 alloantigens were detectable at all stages, whereas paternal antigens first became evident at the 6- to 8-cell stage. No convincing evidence of the presence of alloantigens associated with the H-2 haplotype was found at any stage or on any cell type, suggesting that if these antigens are present they are in low quantity or are masked.

Animals↗
PubMedDate not supplied

Criteria that optimize the potential of murine embryonic stem cells for in vitro and in vivo developmental studies.

Cultured mouse embryonic stem (ES) cells are used for both in vitro and in vivo studies. The uncommitted pluripotent cells provide a model system with which to study cellular differentiation and development; they can also be used as vectors to carry specific mutations into the mouse genome by homologous recombination. To ensure successful integration into the germ line, competent totipotent diploid ES cell lines are selected using a cell injection bioassay that is both time consuming and technically demanding. The prolonged in vitro culture of rapidly dividing ES cells can lead to accumulated changes and chromosomal abnormalities that will compromise the biological function and abrogate germ line transmission of chimeric mice carrying novel genetic mutations. Such in vitro conditions will vary between individual laboratories; for example, differences in the serums used for maintenance. Using a number of different criteria we attempt in this paper to define the parameters that we found to be key factors for optimization of the biological potential of established ES cell lines. The successful integration into the germ line is dependant on acquiring or deriving a competent totipotent mouse ES diploid cell line. In this paper parameters and criteria are defined which we found to be key factors for the optimization of the biological potential of established ES cell lines.

Animals↗
PubMedDate not supplied

Age associated decrease of N-acetyl-beta-glucosaminidase activity in the lens epithelial cells from C57Bl/6 mice.

Lens epithelial cells were isolated from samples of C57BL/6 and CD-1 mice at different ages between 1 and 5 months, and assayed for the level of N-acetyl-beta-glucosaminidase and their ability to replicate in-vitro. The lens epithelial cells from C57BL/6 mice had a reduced replicative potential compared to other normal strains. A statistically significant decrease in active enzyme levels was demonstrated to be associated with increased animal age of C57BL/6 mice.

Acetylglucosaminidase↗