PubMed Health⌕ Search

Biomedical subjects

A L Roskies

Publications and source records attributed to A L Roskies.

7 recordsLinked to original sources
PubMed2001

Topographic-specific axon branching controlled by ephrin-As is the critical event in retinotectal map development.

The retinotectal projection is the predominant model for studying molecular mechanisms controlling development of topographic axonal connections. Our analyses of topographic mapping of retinal ganglion cell (RGC) axons in chick optic tectum indicate that a primary role for guidance molecules is to regulate topographic branching along RGC axons, a process that imposes unique requirements on the molecular control of map development. We show that topographically appropriate connections are established exclusively by branches that form along the axon shaft. Initially, RGC axons overshoot their appropriate termination zone (TZ) along the anterior-posterior (A-P) tectal axis; temporal axons overshoot the greatest distance and nasal axons the least, which correlates with the nonlinear increasing A-P gradient of ephrin-A repellents. In contrast, branches form along the shaft of RGC axons with substantial A-P topographic specificity. Topography is enhanced through the preferential arborization of appropriately positioned branches and elimination of ectopic branches. Using a membrane stripe assay and time-lapse microscopy, we show that branches form de novo along retinal axons. Temporal axons preferentially branch on their topographically appropriate anterior tectal membranes. After the addition of soluble EphA3-Fc, which blocks ephrin-A function, temporal axons branch equally on anterior and posterior tectal membranes, indicating that the level of ephrin-As in posterior tectum is sufficient to inhibit temporal axon branching and generate branching specificity in vitro. Our findings indicate that topographic branch formation and arborization along RGC axons are critical events in retinotectal mapping. Ephrin-As inhibit branching along RGC axons posterior to their correct TZ, but alone cannot account for topographic branching and must cooperate with other molecular activities to generate appropriate mapping along the A-P tectal axis.

Animals↗
PubMed2001

Task-dependent modulation of regions in the left inferior frontal cortex during semantic processing.

To distinguish areas involved in the processing of word meaning (semantics) from other regions involved in lexical processing more generally, subjects were scanned with positron emission tomography (PET) while performing lexical tasks, three of which required varying degrees of semantic analysis and one that required phonological analysis. Three closely apposed regions in the left inferior frontal cortex and one in the right cerebellum were significantly active above baseline in the semantic tasks, but not in the nonsemantic task. The activity in two of the frontal regions was modulated by the difficulty of the semantic judgment. Other regions, including some in the left temporal cortex and the cerebellum, were active across all four language tasks. Thus, in addition to a number of regions known to be active during language processing, regions in the left inferior frontal cortex were specifically recruited during semantic processing in a task-dependent manner. A region in the right cerebellum may be functionally related to those in the left inferior frontal cortex. Discussion focuses on the implications of these results for current views regarding neural substrates of semantic processing.

Acoustic Stimulation↗
PubMed1994

Control of topographic retinal axon branching by inhibitory membrane-bound molecules.

Retinotopic map development in nonmammalian vertebrates appears to be controlled by molecules that guide or restrict retinal axons to correct locations in their targets. However, the retinotopic map in the superior colliculus (SC) of the rat is developed instead by a topographic bias in collateral branching and arborization. Temporal retinal axons extending across alternating membranes from the topographically correct rostral SC or the incorrect caudal SC of embryonic rats preferentially branch on rostral membranes. Branching preference is due to an inhibitory phosphatidylinositol-linked molecule in the caudal SC. Thus, position-encoding membrane-bound molecules may establish retinotopic maps in mammals by regulating axon branching, not by directing axon growth.

Animals↗
PubMed1994

Plasticity in the development of topographic order in the mammalian retinocollicular projection.

The topographically ordered retinocollicular projection in rats emerges from an initially diffuse projection present in neonates through the elimination of aberrantly positioned axons and arbors. We explore developmental plasticity in this process by making partial retinal lesions at birth and determining the topographic mapping of the remaining retina at later ages when the map normally has a mature, retinotopic order. In normal mature rats, DiI focally injected into the retina labels axons that form a dense focus of overlapping arbors at the topographically correct location in the superior colliculus (SC). Similar injections in rats with partial retinal lesions label axons that form two discrete foci of arborizations; one at the topographically appropriate region of the SC and another in the region of the SC deprived of its normal retinal input by the retinal lesion. A focal injection of DiI into the "deprived" SC region retrogradely labels ganglion cells widely scattered in the retina. Therefore, a partial retinal lesion in developing rats does not lead to an orderly expansion of the remaining retinal projection to cover the entire SC, as it does in amphibians and fish following optic nerve regeneration. Rather, in rats, the remaining partial retina forms two distinct, contiguous projections to the SC: a retinotopically ordered one that retains normal topographic relationships and an aberrant, diffusely ordered one to the SC region topographically matched with the lesioned part of the retina. This abnormal persistence of topographically aberrant axons and arbors indicates that competitive interactions between retinal axons drive the remodeling of the initially diffuse retino-collicular projection into a topographically ordered one.

Animals↗