Biomedical subjects

A M Severyns

Publications and source records attributed to A M Severyns.

6 recordsLinked to original sources

PubMed1991

Non-toxic antiseptic irrigation with chlorhexidine in experimental revascularization in the rat.

The effect of different wound irrigation fluids upon femoral arteries and veins was investigated in the rat, using microsurgical techniques. Toxicity was evaluated by microscopical observation after selective staining of histological slides. Povidone-iodine, 10%, proved to be a very irritant solution, provoking an attack on the vascular endothelium and secondary thrombosis. Chlorhexidine at 0.05%, 0.02% and 0.001% was found by contrast to have a very low toxicity which was comparable to physiological saline. Experimental investigation of antiseptic solutions should not only include the determination of the antibacterial effect, but also the potential for cell toxicity, using an irrigation technique.

Animals↗

PubMed1989

[Current trends in experimental peripheral nerve regeneration].

Large posttraumatic defects in the peripheral nervous system need to discover methods to solve the demand of nerve grafts. There is no definite answer now. The authors present a model for nervous regeneration studies. Experiments are performed in the Wistar rat. A venous isograft is used to bridge defects of various size in a divided rat sciatic nerve. The venous tube is a guide for axonal regeneration. In one series, the tube is filled with physiological saline and, in a second one, neonatal Schwann cells are injected in the venous isograft. Results recorded are a combination of quantitative methods: neurophysiology and morphometry. The injection of neonatal Schwann cells is able to stimulate nerve regeneration but not completely.

Animals↗

PubMed1988

[Preliminary study on nerve regeneration through an autologous venous tube].

Large posttraumatic defects in the peripheral nervous system bring up serious problems to the surgeon. There is no definitive answer yet. The authors present a model for nervous regeneration studies; venous autograft was used to bridge defects in a divided rat sciatic nerve; the venous tube guided the regenerating axons towards the distal stump over up to 1.9 cm length. Preliminary studies about nervous regeneration stimulation with autologous Schwann cells are discussed.

Animals↗

PubMed1980

[Immunogenicity of nerve allografts in rats].

The state of sensitization of rats after nerve or skin allograft was studied in vitro by using spleen cells or peripheral blood leukocytes (PBL) as responding cells and particulate alloantigen for stimulation, in culture conditions requiring or not supernatant from mixed lymphocytes culture. It is shown that 2-4 weeks after nerve or skin grafting a small number of PBL generates similar cytotoxic lymphocytic responses. However the state of sensitization induced by the nerve allografts is shorter compared to the one exhibited after skin allografts. On the other hand, no significant differences were observed at the level of the humoral response between both groups of grafted rats.

Animals↗

PubMed1975

[In vitro distinction between primary and secondary cellular response to alloantigens by means of a subcellular antigenic preparation].

Living spleen cells and a subcellular product of spleen cells have been compared, in mice, for their ability to elicit, for a long term culture (4-5 days), a cellular cytotoxic response against alloantigens. Contrary to living spleen cells which could induce the same high level of cytotoxic activity in a primary or in a secondary response, the antigenic preparation was only able to mount a very low primary response while it could render highly cytotoxic a lymphoid population proved to be able to mount in vitro an anamnestic response.

Animals↗

PubMed1975

[Trial of purification and serological characterization of human and murine transplantation antigens].

Human and murine antigens are purified by exclusion chromatography on Sephadex and preparative polyacrylamide gel electrophoresis and their purification stage checked by analytical methods. The study of antigenic preparations consists of electrofocusing, molecular weight estimation and chemical determinations. A cytotoxicity inhibition test with specific alloantisera reveals the antigenic potency. A microtest using 51Cr labelled tumoral cells has been achieved for the analysis of murine preparations.

Animals↗