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Biomedical subjects

A M Soliman

Publications and source records attributed to A M Soliman.

At least 37 records · Page 2Linked to original sources
PubMed1988

Optimizing immunofluorescence for the testing of autoantibodies in inner ear disorders.

A method is described for the immunofluorescent testing of autoantibodies in the sera of patients with vestibulo-cochlear disorders, using fixed decalcified inner ear tissue preparations from guinea pigs. Fixation in cold ethanol and decalcification in EDTa at 4 degrees C preserved the immunological reactivity of the inner ear tissue in use and allowed excellent delineation of its structural details. Counterstaining of the sections with Evans-blue dye aided in a better identification of the inner ear anatomical structures and in a more precise location of the antibodies present. By mounting the sections in p-phenylenediamine-PBS-glycerin solution, the rapid extinction of the fluorescence seen during photomicroscopy was retarded and the intensity of the immunofluorescence was enhanced. Preservation of the slides for documentation was improved by fixing the intermediate layer of the antigen and the labeled antibody in cold ethanol. The inner ear tissue preparations retained their fluorescent staining up to 6 months. Our method is convenient for screening patients with inner ear disorders for autoantibodies against the different cellular elements and for testing the possible presence of antibodies against the inner ear tissue. So far, we believe that the antibodies detected are not tissue (inner ear) specific.

Animals↗
PubMed1988

A comparative immunofluorescent study of fixed decalcified tissue and frozen non-decalcified tissue from the guinea pig cochlea.

The immunofluorescence test (IF) is an important diagnostic laboratory tool for detecting immune-mediated sensorineural hearing loss (SNHL). The results of applying the IF to ethanol-fixed, paraffin-embedded, decalcified sections of guinea pig cochlea as compared with frozen non-decalcified preparations are presented. Sera from ten patients with SNHL were tested in addition to antibody-positive and negative human serum controls. Despite fixation and prolonged decalcification, deparaffinized cochlear sections showed a strong positive reaction with most of the positive serum controls. Anti-endothelial antibodies were detected in the serum of one patient and anti-nuclear antibodies in another. The fine cochlear structure was clearly delineated. However, the decalcified specimens failed to react with one serum model, which revealed a positive fluorescence when incubated with the frozen non-decalcified cochlear preparations. These latter findings denote possible leaching of the antigenic material from the sections. This study further shows that frozen nondecalcified cochlear tissue is a reliable tissue source for the interpretation of the results obtained with IF.

Animals↗
PubMed1988

An improved technique for the study of immunofluorescence using non-decalcified frozen guinea pig cochlea.

A technique for producing frozen sections from the non-decalcified guinea pig cochlea is described. The sections can be utilised for various immunofluorescent studies, including the detection of humoral antibodies against different cellular elements in patients with cochleo-vestibular disorders. The technique assures the preservation of the cochlear architecture and the tissue antigenicity, and it avoids damage to the fine cochlear structural details.

Animals↗
PubMed1988

Changes in the brainstem evoked responses following jugular vein ligation.

Blockage of both jugular veins is often followed by signs of intracranial hypertension. The use of brainstem evoked responses (BSER) in the detection of brainstem compression following jugular vein ligation in guinea pigs was studied. Thirty pigmented guinea pigs were studied. Unilateral jugular vein ligation was performed in 10 animals and bilaterally in 10. Ten animals were controls. The BSER to clicks at 20 db above hearing thresholds before and 6 hours following ligation of one or both jugular veins were recorded. No change in the hearing threshold was observed following jugular vein(s) ligation. However, prolongation of the I-III and III-IV interpeak intervals were observed following both unilateral and bilateral jugular vein ligation. The results suggest that BSER monitoring may be useful in the early detection of brainstem compression following jugular vein ligation in head and neck surgery.

Animals↗
PubMed1987

The use of immunofluorescence in the non-decalcified frozen guinea pig cochlea to detect autoantibodies in inner ear disorders.

Autoantibodies in the serum of patients with inner ear disorders have been detected using the immunofluorescence technique. In the present study the results of the use of frozen sections of non-decalcified guinea pig cochlea in the indirect immunofluorescence test are described. Human sera with positive anti-mitochondrial (AMA), anti-nuclear (ANAS) and anti-nucleolar (ANAN) antibodies were utilized, as well as a negative serum control and sera from three patients suffering from chronic progressive sensorineural hearing loss. The positive reactions obtained were clear and specific. Autofluorescence was minimal and non-specific reactions were negligible. The fine structure of the inner ear tissue and the cellular antigenicity present were well preserved.

Animals↗
PubMed1982

The spinal fluid of acute hepatitis B patients--an occupational hazard?

Spinal fluid and serum samples from 19 patients, with acute viral hepatitis, type B, already known to have HBs antigenemia, were tested for the detection of HBsAg by two techniques; Hemagglutination Inhibition (H.I.) and Enzyme Linked Immuno Sorbent assay (ELISA). HBsAg was detected in all serum samples by both techniques while all spinal fluid samples were free of that antigen except one sample that was found to contain HBsAg by the ELISA technique only. This sample was also proved to contain occult blood as shown by the positive benzidine reaction.

Acute Disease↗
PubMed1981

Isolation and identification of African horsesickness virus from naturally infected dogs in Upper Egypt.

African horsesickness virus was isolated from blood samples of street dogs in Aswan Province in Arab Republic of Egypt. Of six isolated "dog strain" African horsesickness viruses, three viruses designated D2, D6 and D10 have been identified as type 9 African horsesickness virus. Methods of isolation, tissue culture adaptation, serological indentification and typing are described. Horses experimentally infected with dog viruses showed febrile reaction and characteristic clinical and pathological signs of African horsesickness. Reisolation of African horsesickness virus type 9 was achieved from the horses during serial passages.

African Horse Sickness↗