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Biomedical subjects

A Möller

Publications and source records attributed to A Möller.

At least 163 records · Page 9Linked to original sources
PubMed1985

Generation of leukotrienes from normal epidermis and their demonstration in cutaneous disease.

In order to evaluate the significance of chemotactic leukotrienes in cutaneous disease, synthetic leukotriene B4 and its metabolites were examined during in vitro chemotaxis. Leukotriene B4, and less so 20-OH-leukotriene B4, were chemotactic for neutrophils and eosinophils, with a preferential attraction of eosinophils. The responsiveness of human monocytes towards leukotriene B4 was relatively low. Normal cells and cells from different patients varied in their quantitative response. Cells from patients with eczema and T-cell lymphoma tended to migrate less than those from patients with other inflammatory diseases. Leukotrienes are generated from several types of peripheral leukocytes. In order to examine whether resident cells of the skin can also produce these factors, isolated human and murine epidermal cells were examined for their ability to generate leukotriene B4 and leukotriene C4 in vitro. Arachidonic acid, and less so the ionophore A 23187, induced the generation of both types of factors, based on the finding in the bioassay, reverse-phase high-pressure liquid chromatography and radioimmunoassays. The same factors were demonstrated by either or all of these methods in skin biopsies, scales, blisters or suction blisters of patients with psoriasis, pitysiasis rubra pilaris, dyshidrosis, bullous pemphigoid, pressure urticaria, urticaria pigmentosa and drug reactions, but not in Sézary syndrome nor in callus and skin biopsies of normal controls. These findings underline the fact that the leukotrienes are potent inflammatory mediators in diverse skin diseases, but that they are not limited to any specific disease. Furthermore, a relationship between leukotrienes and tissue eosinophilia does not exist.

Animals↗
PubMed1985

A microbiological study of secretory otitis media using an anaerobic technique.

A serious anaerobic sampling and cultivation technique was used in order to obtain representative samples from 45 middle ear effusions in 30 patients with longstanding secretory otitis media (SOM). Despite the fact that an accurate sterilizing procedure was performed, 16% disclosed contaminated samples from the ear canal and tympanic membrane. Among the remaining samples, middle ear bacteria were found in 30% of which Staphylococcus epidermidis and Haemophilus influenzae contributed to the major part. Anaerobes were only occasionally found. The amount of bacteria found was sparse in all cases. It can be concluded that the inflammatory process in SOM is not maintained by anaerobic bacteria within the middle ear cavity.

Bacteria, Anaerobic↗
PubMed1985

[Results of radiotherapeutic optimization within the scope of combined operative-radiologic therapy of urinary bladder cancer].

The results of a prospective randomized clinical study for the comparison of a uniserial irradiation with a biserial irradiation are demonstrated. The investigations were performed on altogether 191 patients. Patients after cystectomy and supravesical by-pass of the urine were excluded. The operative treatment consisted of TUTUR, transvesical tumour resection or partial resection of the urinary bladder. According to the life table method the uniserial irradiation a survival rate of 52% and for the biserial irradiation of 39% after 54 months was calculated.

Aged↗
PubMed1984

Bromination stabilizes poly(dG-dC) in the Z-DNA form under low-salt conditions.

Using circular dichroism studies, Pohl & Jovin (1972) [Pohl, F.M., & Jovin, T.M. (1972) J. Mol. Biol. 67, 375-396] demonstrated that poly(dG-dC) undergoes a salt-dependent conformational change characterized by a spectral inversion. The low-salt form corresponds to the right-handed B form of DNA and the high-salt form to the left-handed Z-DNA helix. Modification of poly(dG-dC) by adding bromine atoms to the C8 position of guanine and the C5 position of cytosine residues stabilized this polymer in the Z-DNA form under low-salt conditions. The guanine residues were found to be twice as reactive as the cytosine residues. With a modification of 38% Br8G and 18% Br5C, the polymers formed a stable Z-DNA helix under physiological conditions. The bromination produced spectroscopic features very similar to poly(dG-dC) in 4 M NaCl. However, bromination did not freeze the Z structure as was shown by ethidium bromide intercalation studies. Addition of the dye favored an intercalated B-DNA form. The conversion of B- to Z-DNA leads to profound conformational changes which were also seen by a reduced insensitivity to various exo- and endonucleases. Comparative studies showed that the brominated polymers have a high affinity to nitrocellulose filters. In 1 M NaCl, there was virtually no binding of B-DNA, but a substantial binding of Z-DNA was found even at rather low levels of bromination.(ABSTRACT TRUNCATED AT 250 WORDS)

Bromine↗
PubMed1984

[Severe migraine accompagnée with computer tomographic findings].

Migraine accompagnée is associated with cerebral signs of irritation or failure, aphasic or paraphasic disturbances and Jacksonian attacks which appear together with the migraine attack. Present-day ideas on the pathogenesis of migraine headache indicate that such cerebral focal signs should be ascribed to vessel-dependent changes with transitory ischaemia. The article reports on a 24-year-old woman patient who had three Jacksonian attacks within a severe attack of migraine besides neurological signs originating from various cerebrovascular areas, as well as certain mental anomalies; this patient had a previous history of migraine with familial affiliations. The computerized tomogram, produced on the 5th day of disease, showed frontally-paramedially a zone with unsharp outlines without enhancement after administration of a contrast medium. All neurological signs, including the Jacksonian attacks, gradually receded in the course of time. Extensive additional diagnosis was performed, including angiography, to exclude any other underlying disease which could have been made responsible for the observed symptoms.

Adult↗
PubMed1984

Pleural effusion. Use of the semi-supine position for radiographic detection.

Chest radiographs of 100 routinely examined patients were evaluated to study the effectiveness of the oblique semi-supine position when attempting to identify pleural effusion. Comparisons were made to images obtained with the patient in the lateral decubitus position. Radiography was performed without noticeable discomfort for 62 patients in the lateral decubitus position and for 97 patients in the oblique semi-supine position. The visualization of free pleural fluid was not affected by the different body positions, but demonstration of encapsulated fluid was superior in views obtained with the patient in the oblique semi-supine posture. For most individuals with suspected pleural effusion, the oblique semi-supine position is preferable to the lateral decubitus position.

Adolescent↗
PubMed1983

Z-DNA-specific antibodies in human systemic lupus erythematosus.

Naturally occurring antibodies to left-handed Z-DNA have been shown to be present in the sera of human patients with systemic lupus erythematosus (SLE). These antibodies are of two types. One type reacts with both denatured DNA and Z-DNA. The other type is specific for Z-DNA and remained in the serum after removal of the cross-reactive antibody by extensive absorption on a denatured DNA affinity column. The antibodies appear to be specific for SLE and do not appear frequently in other rheumatic diseases. The presence of the antibody in SLE is correlated with the clinical manifestations of the disease, in parallel with antibodies to native and denatured DNA.

Antibody Specificity↗
PubMed1983

Production of histolytic enzymes by a combination of oral bacteria with known pathogenicity.

Eight oral bacterial strains, isolated from an infected root canal, have been investigated for their capacity to produce histolytic enzymes. The determination was performed using methods espoused by two different principles. Eleven out of 12 enzymes examined were demonstrated in the "eight-strain collection". In no single bacterial strain were all enzymes revealed. It was suggested that the pathogenicity of the bacterial strains, singly or in combination, was not solely dependent on the production of these enzymes. The histolytic enzymes may have a potentiating role on other pathogenic factors in infectious diseases.

Bacteria↗
PubMed1982

Monoclonal antibodies recognize different parts of Z-DNA.

Brominated poly(dG-dC) . poly(dG-dC), which forms a stable Z-DNA helix under physiological salt conditions, was used as an antigen to produce monoclonal antibodies from mice. The anti-Z-DNA antibodies were specific for the Z conformation as was shown by direct binding studies and a competitive solid phase assay. Competition could only be seen by brominated poly(dG-dC) . poly(dG-dC) under low and high salt conditions and unmodified poly(dG-dC) . poly(dG-dC) under high salt conditions. No binding could be seen to a number of other polynucleotides, including native and denatured DNA. Among the anti-Z-DNA monoclonal antibodies, examples differed in specificity: binding of one monoclonal antibody was blocked by methylation of cytosine; whereas the other antibody reacted equally well with the unmodified polymer or methyl cytosine-containing polymer in high ionic strength. The two antibodies are specific for different localized features of Z-DNA structure.

Animals↗
PubMed1982

[Zoster encephalitis without rash: report of two cases (author's transl)].

Two previously healthy men, aged 54 and 41 years, fell ill with headaches and increased fatiguability, one also with vomiting, the other with fever, transitory visual disturbances and slight weakness of the left hand. Both of them had a stiff neck and clouded consciousness. The EEG had moderate to severe dysrhythmia, predominantly over the temporal area, CSF showed an increased cell count of 1000/3, predominantly lymphocytes, and increased protein. The younger patient also had global aphasia and the computed tomography indicated an area of decreased density in the left temporal region. In the CSF there were locally produced IgG. The clinical findings were similar to those of herpes encephalitis, but were milder and regressed more quickly. Severe months later only a few minor organic behavioural changes were present. Antibody findings in CSF and serum suggest varicella-zoster virus as the causative agent, although in both instances no rash was observed throughout the entire period of observation.

Adult↗
PubMed1982

Isolation of Drosophila proteins that bind selectively to left-handed Z-DNA.

An affinity column for isolating Z-DNA binding proteins was made by attaching brominated poly(dG-dC) to Sephadex. Proteins from Drosophila nuclei were prepared and those that could bind to Escherichia coli B-DNA were removed from the solution. The remaining proteins were passed over the Z-DNA affinity column and then eluted with NaCl. Using both direct and competitive filter binding assays, we found that the eluted proteins bind to brominated poly(dG-dC) (Z-DNA) and poly(dG-m5dC) but not to poly(dG-dC) (B-DNA), native or denatured E. coli or calf thymus DNA, or brominated oligonucleotides. The proteins also bind to negatively supercoiled plasmids carrying Z-DNA sequences but not to relaxed or linearized plasmids in which the Z-DNA conformation is no longer present. Gel analysis reveals a mixture of several large proteins up to approximately 150,000 daltons.

Binding, Competitive↗