Cardiovascular lesions in the selectively-bred group of spontaneously hypertensive rats with severe hypertension.
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Adrenal Glands↗
Biomedical subjects
A Ooshima
Publications and source records attributed to A Ooshima.
Genetic analysis of blood pressure in spontaneously hypertensive rats.
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Animals↗
Alterations in ultrastructure and c-met expression in a case of ocular epithelial dysplasia following topical mitomycin C treatment.
PURPOSE: Topical mitomycin C (MMC) administration is reportedly effective in treating ocular surface neoplasms such as squamous cell carcinoma. We treated a case of ocular epithelial dysplasia that had spread too diffusely to be completely removed. We examined the ultrastructure of and c-met (hepatocyte growth factor receptor) expression in dysplastic epithelial cells from this case to evaluate the efficacy of MMC treatment. METHODS: Specimens of dysplastic epithelial tissue from the corneo-limbal region of a 62-year-old man were obtained before and after topical application of MMC. Specimens were examined ultrastructurally and immunohistochemically with an antibody against human c-met. RESULTS: Following topical application of MMC, the dysplastic epithelium exhibited multilayered epithelial cells similar to those seen before treatment. However, ultrastructural examination showed tight interdigitation between neighboring cells, with no intercellular spaces. Also, the marked immunoreactivity to c-met in the dysplastic epithelial cells before MMC treatment was decreased after treatment. CONCLUSIONS: Ultrastructural observations indicated a restoration of epithelial cellular differentiation following MMC application. The expression of c-met protein was also reduced. Thus, topical MMC was effective in treating epithelial dysplasia of the ocular surface, with no recurrence 15 months post-therapy.
Administration, Topical↗
Corneal epithelial basement membrane after experimental anterior stromal puncture in guinea pigs: immunohistochemical study.
We investigated alterations in the immunolocalization of the components of epithelial basement membrane (BM), type IV collagen, and laminin in guinea pigs subjected to anterior stromal puncture (ASP) of the cornea performed with a standardized needle. Localization of BM components beneath the corneal epithelium was indicated by laminin immunoreactivity. The BM was interrupted by needle punctures immediately after ASP. During healing, type IV collagen immunoreactivity was detected transiently in the BM of some of the ASP-treated corneas, but no reactivity was observed in normal epithelial BM. Development of type IV collagen immunoreactivity was probably caused by an alteration of the alpha-chains or by an unmasking of the antigenicity of this collagen type, which may be related to an increase in the adhesiveness of the epithelium following ASP.
Animals↗
Immunohistochemical identification of proteoglycan types in fibrotic human capsules with intraocular lens implants.
Lens capsules become fibrotic after cataract extraction. A variety of extracellular matrix (ECM) components accumulate on these capsules in association with the proliferation of lens epithelial cells. To provide a better understanding of the process of capsular fibrosis, we assessed the types of proteoglycans (PG) in human lens capsules with intraocular lenses (IOL). Lens capsules containing IOLs were removed from 1 patient with proliferative vitreoretinopathy and 1 patient with proliferative diabetic retinopathy. After treatment with chondroitinase ABC, tissue sections were processed for immunohistochemical detection of the proteoglycans including chondroitin, large PG, chondroitin 4-sulfate PG, chondroitin 6-sulfate PG, dermatan sulfate PG, and keratan sulfate PG. Extracellular matrix was found on the inner surface of the capsular bag. In association with what appeared to be proliferating lens epithelial cells, each of the six types of PG was present in the ECM on the capsules. All six types of PG might be involved in the fibrosis and opacification of lens capsules after extraction of the cataract and implantation of the IOL.
Antibodies, Monoclonal↗
Pathological findings in lens capsule and silicone intraocular lens extracted from eye with chronic infectious endophthalmitis.
The lens capsule and silicone intraocular lens (IOL) extracted from the eye of a patient with chronic infectious endophthalmitis was examined histopathologically to evaluate the pathology of a capsule and IOL surfaces in the presence of bacterial infection. A 69-year-old man developed an infection in his right eye 4 months after phacoemulsification and aspiration of a cataract and implantation of a silicone IOL. During vitrectomy, the capsule and IOL were extracted and processed for light or scanning electron microscopy. Cryosections of the capsule were subjected to Gram staining and immunohistochemical tests for extracellular matrix components. The lens capsule contained an accumulation of extracellular matrix, including collagen types and fibronectin. A colony of Gram-positive rod bacteria was detected inside the capsular bag. Scanning electron microscopy failed to detect any microorganisms on the IOL surface. Histological examination of cryosections of the extracted capsule confirmed the presence of infection during surgery even though preoperative cultures of intraocular fluid were negative. Immediate antibacterial treatment could be initiated.
Aged↗
Stretch-induced collagen synthesis in cultured smooth muscle cells from rabbit aortic media and a possible involvement of angiotensin II and transforming growth factor-beta.
Mechanical strain reportedly stimulates the synthesis of collagen in vascular smooth muscle cells (SMCs). The present study was designed to investigate a possible involvement of angiotensin II (Ang II) and transforming growth factor (TGF)-beta in stretch-induced collagen synthesis of cultured SMCs derived from the rabbit aortic media. SMCs were cyclically stretched at a rate of 10% elongation and 30 cycles/min for 24 h using the Flexercell strain unit (Flexcell International Corp., McKeesport, Pa.). A two-fold increase in collagen synthesis and a concurrent increase in total protein synthesis were noted in stretched SMCs. Concentration of immunoreactive Ang II in the conditioned medium was elevated under the mechanical strain. Stretch-induced collagen and total protein synthesis were inhibited by either a selective antagonist to Ang II (saralasin), an angiotensin I-converting enzyme inhibitor (captopril) or an antisense oligonucleotide for angiotensinogen mRNA. An elevated secretion of TGF-beta, both active and latent forms, was found in the medium of stretched SMCs. Saralasin inhibited the stretch-induced secretion of TGF-beta from SMCs. Stretch-induced collagen and total protein synthesis was further inhibited by either an anti-TGF-beta1 neutralizing antibody or an adenovirus-mediated transfer of a truncated TGF-beta type II receptor. Elevated expression of collagen alpha1(III) chain and TGF-beta1 mRNAs, and its reversal by saralasin were also demonstrated in stretched SMCs. Results indicate that the stretch-induced collagen and total protein synthesis appears to be mediated via an autocrine-paracrine mechanism of Ang II and TGF-beta released from SMCs.
Angiotensin II↗
Immunolocalization of c-Fos and c-Jun in rat ocular surface epithelium.
We immunohistochemically located c-Fos and c-Jun, the major components of transcription factor activator protein 1 (AP1), in normal epithelia of rat cornea and conjunctiva to determine the expression of these genes in the ocular surface epithelia. Immunoreactivity for c-Fos was detected in the nuclei of basal cells of the limbal and conjunctival epithelia, while that for c-Jun was detected in the cytoplasm of the cells of these epithelia. The corneal epithelium lacked immunoreactivity for either protein. AP1 may have an important role in the maintenance of homeostasis of limbal and conjunctival epithelia.
Animals↗