PubMed Health⌕ Search

Biomedical subjects

A Panà

Publications and source records attributed to A Panà.

At least 55 records · Page 3Linked to original sources
PubMed1986

Preliminary characterization of a fast-growing strain of human hepatitis A virus.

A fast-growing strain of Human Hepatitis A (HHA) virus was selected by progressively shortening by the time between serial passages of the isolate in the simian cell line Frp/3. The virus so selected infected 90-95% of the cells in 7-10 days, and developed a strong cytopathic effect (CPE). The synthesis of HHA-specific antigens and the CPE were neutralized simultaneously by standard anti-HHA sera. This fast-growing strain has the characteristics of a picornavirus.

Animals↗
PubMed1986

Comparison of indirect immunofluorescence and radioimmunoassay for detecting antibodies against hepatitis A virus of the IgG and IgM classes.

Indirect Immunofluorescence test (IIF) and Radioimmunoassay (RIA) were compared for determining anti-Hepatitis A virus (HAV) IgG and IgM. 142 sera were tested for anti-HAV IgG and 16 for anti-HAV IgM, both with IIF and RIA techniques. The correlation between the results reached 98.6% for anti-HAV IgG and 93.75% for anti-HAV IgM detection, confirming the specificity and sensibility of IIF.

Adult↗
PubMed1986

Immunological responses of patients with recurrent herpes genitalis.

Immunological responses of patients with recurrent herpes genitalis (RHG) were assayed in comparison to those of patients affected by recurrent herpes labialis (RHL) and to those of normal seropositive and seronegative controls. Total T cells were normal while a significant (p less than 0.001) reduction of cells with helper phenotype was found only in people with RHG, who were also lacking specific cell-mediated immunity (CMI) to herpes simplex virus (HSV) antigen. HSV-type 2 was demonstrated in their active lesions. Number of human NK-1 positive (HNK-1 +) cells was increased in RHG showing an inverse correlation with depressed natural killer (NK) activity. These abnormalities were found irrespective or not if tests were carried out during the active phase of the disease or during quiescence and were confirmed during the 2-year follow-up period to which patients were submitted. Similar abnormalities were found in RHL only during the active phase and reversed to normal during quiescence. Our data indicate the presence of specific cellular and NK unresponsiveness in subjects with severe and long-lasting herpes genitalis.

Adolescent↗
PubMed1985

Effect of amantadine on rhabdovirus infection.

Enveloped viruses enter host cells by fusion or viropexis. The latter mechanism is the prevalent entry pathway of rhabdoviruses into susceptible cells. Amantadine, a lysosomotropic agent, inhibits the multiplication of various groups of viruses. The effect of this drug was investigated on vesicular stomatitis virus and rabies fixed virus strain replication in fibroblasts. Amantadine was added to cells before, during and after infection to detect the phase of viral replication affected by the drug. Cells were inoculated with viruses at 4 degrees C and the incubation temperature was progressively raised to 37 degrees C in order to observe the effect of amantadine on attachment and early stages of viral replication. Experimental results indicated that the compound inhibited rhabdovirus infection in CER cells. Viral attachment and penetration did not appear to be affected by the drug, while later steps were inhibited, probably at the level of uncoating when the virus is released from the lysosomes into the intracytoplasmic compartment.

Amantadine↗
PubMed1984

Prevalence of hepatitis A antibodies in Italy.

Prevalence of hepatitis A antibodies among 5005 naval recruits aged 18-26 from all parts of Italy was almost double in the south and islands (83.3%) as compared to the north and central regions (45.2%). Social-demographic variables such as education and number of siblings are associated with prevalence of anti-HAV. Hepatitis A infection is still an early acquired infection in Italy, particularly in the south where among the 18-26-year-old recruits with education limited to lower middle school or less and eight siblings or more, prevalence of anti-HAV was 95%. Good correlation exists in Italy between prevalence of anti-HAV and incidence of reported cases of hepatitis.

Adolescent↗
PubMed1983

Hepatitis B in two Italian General Hospitals.

A study to evaluate the extent of Hepatitis B among Hospital Staff in two hospitals in central Italy, blood tested 1212 individuals (80.2% of the staff). 33.7% of staff tested was positive to at least 1 marker of prior B hepatitis infection. The study confirms that B hepatitis is an occupational hazard for those exposed to blood. Specific risks in terms of odds ratios are estimated to be about 2.0 for professional nurse compared to other nursing categories, about 1.7 for staff in high risk departments compared to low risk departments and about 5.0 for professional nurses in high risk departments compared to clerks or nurses aides in low risk departments. The computation of attributable risk indicates that working in a hospital contributes only a minor fraction of the total national burden of this disease. However, immunization of hospital workers in Italy can prevent every year as many as 6,500 new Hepatitis B infections in this high risk group.

Adult↗
PubMed1983

Prevalence of hepatitis B markers in Italy.

Among 5005 Navy recruits aged 18-26 years from all sections of Italy, whose blood was tested in 1981, regional prevalence of serologic markers for hepatitis B was higher in southern Italy and in the Italian islands as compared with northern Italy and central Italy (4.3% vs. 2.3% for hepatitis B surface antigen (HBsAg) and 23.5% vs. 10.5% for any B marker). Social-demographic variables such as region, family size, and education were associated with hepatitis B as were the more traditionally associated variables related to blood or medical care such as history of intravenous injection, history of blood transfusion, or exposure to hospital. Number of siblings was positively related and educational attainment inversely related to prevalence of hepatitis B markers. The prevalence of HBsAg among Italian males aged 18-26 years with residence in southern Italy or in the Italian islands, whose education was limited to lower middle school, and who were from a family of eight siblings or more, is 14.6%. These are among the highest prevalence rates for hepatitis B antigen ever reported for a general population group in Europe. Good correlation exists between incidence of reported cases of hepatitis and prevalence of markers in the different regions of Italy.

Adolescent↗
PubMed1983

Activity of human serum lipoproteins on the infectivity of rhabdoviruses.

The inhibiting activity of various human serum lipoprotein classes and their lipid components on the infectivity of rhabdoviruses has been studied. The research has been carried out according to different experimental procedures on both the vesicular stomatitis virus (Indiana strain), and the fixed rabies virus (CVS strain). The results obtained have shown an inhibition of the infectivity of VSV and CVS, mainly linked to the very low and low density lipoprotein classes. It has also been demonstrated that the inhibitors act directly on viruses causing a decrease in their attachment to host cells.

Humans↗
PubMed1983

Preliminary study on fixed rabies virus strain "Roma". I: Adaptation on BHK21/S13 cells.

In this study the attempts to adapt Roma fixed rabies virus to BHK21/S13 cells and human diploid fibroblasts, for a better characterization of the virus, are reported. BHK21 cells were infected with the virus and maintained in culture for more than 100 passages. A persistently infected culture by rabies virus was obtained. Attempts to propagate virus in human diploid fibroblasts were unsuccessful.

Adaptation, Physiological↗
PubMed1982

Cell-mediated immunity in human cytomegalovirus infection.

The direct leukocyte migration inhibition test, in response to cytomegalovirus stimulation, was used to study cell-mediated immunity in a group of children with cytomegalovirus infection. The test was impaired in children with chronic disease associated with cytomegaloviruria. In those cases with no viruria at the moment of the test, leukocyte migration inhibition was normal. Our data suggest that the acquired chronic cytomegalovirus infection may be sustained by a state of specific cellular desensitization, as already demonstrated for congenital infection.

Cell Migration Inhibition↗
PubMed1978

Specific cellular unreactivity during cytomegalovirus infection in man.

The specific cellular immune response against Cytomegalovirus (CMV) was studied by the Leucocyte Inhibitory Factor (LIF) production test in three groups of subjects: 7 healthy individuals with no CMV antibodies, 4 with antibodies to CMV but without evidence of active infection, and 2 children with clinical CMV infection. Our results show that LIF production in presence of CMV is high in normal seropositive subjects, while it is strongly deficient in patients with active infection, despite a good humoral response to the virus. In one child with congenital infection, a low number of E rosette forming cells was also revealed. We suggest that CMV infection can induce, at least during infancy, a state of specific cellular unresponsiveness to the virus. When the infection is congenital, a generalized depression of the cellular immune system may develop.

Antibodies, Viral↗
PubMed1976

Prevalence in the Rome healthy population of antibodies to a human Polyoma - virus (BK - strain).

A serological study of determine the prevalence of infections caused by human Polyoma-virus (BK strain) among the healthy population of Rome has been performed. Such virus was originally isolated from a patient undergoing a kidney transplantation, and later found in patients undergoing immunosuppressive therapy. Previous serological studies showed high incidences of infections, caused by this virus, in human populations. Other viruses, antigenically related, were found in patients suffering from progressive multifocal leukoencephalopathy. Serum samples coming from 482 healthy subjects classified by age and 100 cord blood serum samples were analyzed for antibody titers using haemoagglutination-inhibition and complement fixation tests. High incidence of infection caused by BK virus in the Roman population was found: specifically the haeomagglutination-inhibition test resulted positive in 65.2% of the samples. A remarkable similar trend between the results obtained with both the techniques was observed, although differences in antibody serum levels were present. The infections, which are not transmitted during the intrauterine life, are first noted at about two years of age. The Authors finally briefly discuss the possible epidemiological meaning of the spreading of this infection.

Adolescent↗