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A Remy

Publications and source records attributed to A Remy.

8 recordsLinked to original sources
PubMed1998

Biochemical separations by continuous-bed chromatography.

Innovations in column-packing media for biomolecule purification have progressed from large spherical, porous polysaccharide beads to advanced polymeric supports. Continuous-bed technology is a radical new technology for chromatography based on the polymerization of advanced monomers and ionomers directly in the chromatographic column. The polymer chains form aggregates which coalesce into a dense, homogeneous network of interconnected nodules consisting of microparticles with an average diameter of 3000 A. The voids or channels between the nodules are large enough to permit a high hydrodynamic flow. Due to the high cross-linking of the polymer matrix, the surface of each nodule is nonporous yet the polymeric microparticles provide a very large surface area for high binding capacity. This paper will demonstrate the properties and advantages of using a continuous bed support for high resolution biomolecule separations at high flow-rates without sacrificing capacity.

Animals↗
PubMed1998

[A peculiar mechanism of hydrocephalus: the "water-hammering" effect].

BACKGROUND AND PURPOSE: Aneurysms of the basilar artery can cause hydrocephalus due to compression of the third ventricle or the sylvian aqueduct. The observation of a particular case led to discuss another possible mechanism of hydrocephalus. CLINICAL PRESENTATION AND RADIOLOGICAL STUDIES: An aneurysm of the basilar artery was revealed by an ischemic stroke in a 65-year-old man. Hydrocephalus developed during the following months. The MRI studies showed that it could not be explained merely by a permanent compression. However, the patient improved clearly after a ventriculo-peritoneal derivation. CONCLUSION: The hydrocephalus could be explained by a "water-hammering" effect due to the pulsating blood in the ectatic vessel, which created a cerebrospinal fluid outflow impairment through the third ventricle.

Aged↗
PubMed1997

[Late cerebral radionecrosis of cystic aspect].

Two cases of delayed cerebral radionecrosis with cystic presentation are reported. The patients had received radiation therapy for a cutaneous tumor 16 and 12 years before. Surgical extirpation of the cerebral lesion permitted to confirm the diagnosis and to treat the patients with success. A cystic aspect has been reported rarely in the neuroradiologic descriptions of the cerebral radionecrosis in the literature. Its mechanism is discussed. The treatment of the delayed cerebral radionecrosis is not clearly established, but a cystic presentation is an argument for surgery.

Adult↗
PubMed1991

Immunocytochemistry of the taurine biosynthesis enzyme, cysteine sulfinate decarboxylase, in the cerebellum: evidence for a glial localization.

Immunocytochemistry of cysteine sulfinate decarboxylase was developed in the cerebellum with an antiserum that we have recently produced using as immunogen a homogeneous fraction purified about 2000-fold from liver. In the rat, this antiserum immunoprecipitated the enzymatic activity from brain, labeled one band (molecular weight = 51,000) on immunoblots of an enriched fraction from brain but none with a brain crude extract and thus appeared to be specific. In the cerebellum numerous immunolabeled cells were found in the white matter that were typically arranged in rows like oligodendrocytes. A few immunolabeled cells were scattered in the granular layer. Around the Purkinje cells numerous small satellite cells were immunostained that sent faintly labeled radial fibers through the molecular layer. These cells were thus identified as Golgi epithelial cells with their Bergmann fibers. Purkinje cells were not labeled either at the cell body or at the nerve ending levels. In the molecular layer no cells were found consistently and convincingly immunolabeled that could correspond to the stellate cells. No immunopositive punctae typical of the interneuron nerve endings were ever observed. A significant cysteine sulfinate decarboxylase activity was also measured in glial cell fractions enriched in oligodendrocytes and astrocytes respectively. The glial localization of taurine biosynthesis enzyme in the cerebellum does not support the hypothesis that it could be involved in neurotransmission, but rather suggests that taurine is manufactured by glial cells and accordingly may play a physiological role in relation to glial function.

Animals↗
PubMed1990

Specific antiserum and monoclonal antibodies against the taurine biosynthesis enzyme cysteine sulfinate decarboxylase: identity of brain and liver enzyme.

Cysteine sulfinate decarboxylase (CSD), the putative biosynthetic enzyme for taurine, was purified 1,800-fold with a 1% yield from rat liver, where it was found to be 20-fold enriched compared with brain. The final fraction was homogeneous, as ascertained through sodium dodecyl sulfate-polyacrylamide gel electrophoresis and reverse-phase HPLC. An antiserum was raised in the rabbit that (a) quantitatively immunoprecipitated CSD activity and (b) immunolabeled only one band (MW = 51,000) on an immunoblot from liver homogenate. Monoclonal antibodies were also raised that recognized the CSD protein and immunolabeled the same 51-kilodalton protein on an immunoblot from liver homogenate. In a brain extract, two CSD activities had been previously found and named CSDI and CSDII, according to their chromatographic elution patterns. We have compared the properties of CSDI from brain--the most likely enzyme involved in the biosynthesis of taurine in the brain, according to previous investigations-and CSD from liver: Both activities (a) were similarly eluted on ion-exchange and hydroxyapatite chromatographies, (b) showed the same elution pattern on gel filtration with an apparent native molecular weight of approximately 63,000, and (c) were immunoprecipitated in a strictly identical manner by the antiserum against liver CSD. Moreover, this antiserum as well as the monoclonal antibodies immunolabeled a single band (51 kilodaltons) on an immunoblot from brain CSD-enriched fraction or liver fraction. All these data show that CSDI from brain and liver CSD are the same monomeric enzyme. They also indicate that a specific antiserum against rat liver CSD has been raised that can be used for immunocytochemical visualization of CSD-containing cells in the brain.

Animals↗
PubMed1984

[The Marcus Gunn phenomenon].

Ten cases of the Marcus Gunn phenomenon are reported. This synkinesis presents as an involuntary raising of the upper eyelid, often exhibiting ptosis at rest, when the lower jaw drops or deducts towards the opposite side. Hypotheses proposed to explain its mechanisms are reviewed, and the differential diagnosis is discussed in relation to the Marin Amat syndrome, the significance of which is considered. In addition, a case of rarely reported faciopalpebral synkinesis is also reported, the patient presenting involuntary raising of an upper eyelid during spontaneous or voluntary smiles.

Adolescent↗