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PubMed · 9741095

Biochemical separations by continuous-bed chromatography.

T L Tisch·R Frost·J L Liao·W K Lam·A Remy·E Scheinpflug·C Siebert·H Song·A Stapleton
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Abstract

Innovations in column-packing media for biomolecule purification have progressed from large spherical, porous polysaccharide beads to advanced polymeric supports. Continuous-bed technology is a radical new technology for chromatography based on the polymerization of advanced monomers and ionomers directly in the chromatographic column. The polymer chains form aggregates which coalesce into a dense, homogeneous network of interconnected nodules consisting of microparticles with an average diameter of 3000 A. The voids or channels between the nodules are large enough to permit a high hydrodynamic flow. Due to the high cross-linking of the polymer matrix, the surface of each nodule is nonporous yet the polymeric microparticles provide a very large surface area for high binding capacity. This paper will demonstrate the properties and advantages of using a continuous bed support for high resolution biomolecule separations at high flow-rates without sacrificing capacity.

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AnimalsCattleChromatography, Ion ExchangeHumansMercuryMicroscopy, Electron, ScanningMuramidaseOocytesOvalbuminParticle SizePolysaccharidesPorosityProteinsRatsReproducibility of ResultsXenopus laevisJournal Article

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T L Tisch, R Frost, J L Liao, W K Lam, A Remy, E Scheinpflug, C Siebert, H Song, A Stapleton. 1998-08-07. Biochemical separations by continuous-bed chromatography.. https://doi.org/10.1016/s0021-9673(98)00195-2

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