PubMed Health⌕ Search

Biomedical subjects

A Schultz

Publications and source records attributed to A Schultz.

At least 145 records · Page 8Linked to original sources
PubMed1986

Thiazides in the prophylactic treatment of recurrent idiopathic kidney stones.

In a double-blind prospective clinical study patients with at least two verified episodes of urolithiasis, but stone-free at the time of inclusion in the study, were subjected to prophylactic treatment with either bendroflumethiazide (2.5 mg) + 573 mg potassium chloride 3 times a day, or placebo. The material included a total of 22 patients, all males, aged 20-49 years. We found a clear tendency to reduced stone formation in the group treated with the active drug (P less than 0.01). Calcium excretion was reduced by 25-50% in 7 of 12 patients treated with thiazide.

Adult↗
PubMed1985

Maturation of murine leukemia virus env proteins in the absence of other viral proteins.

A mutant of Akv which produces env but no detectable gag or pol products (A. Rein, D. R. Lowy, B. I. Gerwin, S. K. Ruscetti, and R. H. Bassin, J. Virol. 41, 626-634 (1982] was examined for maturation of env gene protein products. In comparison with wild-type Akv, the mutant AK 71 synthesizes gPr85env and produces gp70 and Prp15E in normal amounts and with normal kinetics. Cell surface gp70 was found alike in the mutant and wild type. However, cleavage of Pr15E to p15E did not occur in the mutant. This final cleavage step of AK 71 Prp15E could be made to occur by superinfecting cells containing the mutant with baboon endogenous virus. Thus, unlike earlier steps, this final step in maturation of the env gene product appears to require gag or pol gene products. It is proposed that the virus-encoded protease is required for this last step.

Animals↗
PubMed1985

Standing and supine Cobb measures in girls with idiopathic scoliosis.

The standing Cobb measure in a group of 287 girls with idiopathic scoliosis at the start of treatment was in the mean approximately 9 degrees larger than the supine Cobb measure. This mean standing-supine difference was essentially independent of curve severity expressed by the supine Cobb measure. Because of this, when the difference was expressed as a percentage of the supine Cobb measure, the mean values of this ratio decreased substantially with increasing curve severity. The mean difference decreased only slightly with age through adolescence, indicating that spine lateral curves stiffen little as patients mature. The standard deviations in the standing minus supine Cobb differences were approximately 6 degrees in the majority of the age and severity groupings studied. Ninety-three percent of these patients had standing-supine Cobb measure differences that were between 0 degrees and 20 degrees.

Adolescent↗
PubMed1985

Fibrin adhesive sealing of ureter after ureteral stone surgery. A controlled clinical trial.

In a prospective randomized study 8 patients were peroperatively allocated to closure of ureterotomies with a fibrin adhesive (Tisseel) and 8 patients to closure with Dexon sutures to evaluate whether the duration of postoperative urinary leakage could be diminished by the use of fibrin adhesive. The median drainage period was 6.5 days (range 3-15 days) and 5.5 days (2-16 days), respectively. Closure of ureterotomies with a fibrin adhesive cannot be recommended.

Adult↗
PubMed1984

Different recombinant murine leukemia viruses use different cell surface receptors.

Retroviruses can be grouped by viral interference measurements into classes which use common cell surface receptors. We previously tested a large number of isolates of mink cell focus-inducing (MCF) murine leukemia viruses (MuLVs), and reported that all of them share a distinct receptor on NIH/3T3 cells (A. Rein, Virology 120, 251, 1982). We now extend this generalization to several additional recombinant isolates, including two (SL3-2 and GPA-V2) which would not be considered MCFs on the basis of host-range data. We note the superiority of interference tests, based on positive, unambiguous data, over host-range tests for virus classification. We also show that in contrast to the MCFs, which are all derived from ecotropic MuLVs, a recombinant derived from wild mouse amphotropic MuLV (S. Rasheed et al., Int. J. Cancer 29, 345, 1982) uses a unique receptor on NIH/3T3 cells. This suggests that (a) mouse cells contain more than one type of endogenous env sequence; and (b) there is some specificity in the generation of recombinants, since ecotropic MuLVs appear to give rise only to MCFs, while amphotropic MuLV has generated a distinct type of recombinant. It also represents a second case (in addition to the MCFs) in which an env gene recombinant is more pathogenic than its exogenous parent. We also show that xenotropic MuLV does not interfere with MCFs in NZB mouse cells; thus, despite the close homology between MCF and xenotropic env sequences, the gp70 of xenotropic MuLV appears to have no detectable affinity for the MCF receptor.

Animals↗
PubMed1984

Myristylation of gag-onc fusion proteins in mammalian transforming retroviruses.

Four cell lines producing transforming proteins encoded by three mammalian oncogenes (fes, abl, and ras) were investigated for incorporation of [3H]myristate into gag-onc fusion proteins. Using 5-min pulse-labelings, fusion proteins of Abelson murine leukemia virus, Gardner-Arnstein strain of feline sarcoma virus (FeSV), and Snyder-Theilen strain of FeSV were shown to be myristylated. In a 4-hr pulse, p29gag-ras of rat sarcoma virus (RaSV) was also shown to incorporate radiolabel. The fatty acid was recovered from this labeled protein by acid hydrolysis, and identified by reverse-phase thin-layer chromatography to be [3H]myristic acid. The results indicate that substitution of viral gag sequences by cellular oncogene sequences does not abolish their ability to become post-translationally modified by this long chain fatty acid (A. Schultz and S. Oroszlan, J. Virol. 46, 355-361). It is assumed that in the fusion proteins the myristyl moiety is linked through an amide linkage to the amino-terminal glycine as previously found for several retroviral gag precursor polyproteins (L. E. Henderson, H. C. Krutzsch, and S. Oroszlan, Proc. Natl. Acad. Sci. USA 80, 339-343). The possible role of myristylation of transforming proteins is discussed.

Amino Acid Sequence↗
PubMed1984

Direct vision internal urethrotomy. A critical review of 365 operations.

Internal urethrotomy under direct vision for urethral strictures in the male was employed on 369 occasions in 225 patients during a 4-year period. A retrospective review of results showed an overall cure rate of 77% but the cure rate after each individual operation was less than 50%. Operations for recurrence carried a significantly lower cure rate than initial operations but even after several recurrences there were reasonably good results considering the relatively minor procedure. An active post-operative follow-up employing routine urethroscopy showed significantly better results than mictiographic follow-up. The post-operative period of catheterisation and positive urine culture at the time of follow-up had no significant influence on the results.

Adult↗