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Biomedical subjects

A V Grishchenko

Publications and source records attributed to A V Grishchenko.

8 recordsLinked to original sources
PubMed2000

Effect of mesodiencephalic modulation on small intestinal mucosa after massive blood loss in rats.

Effect of mesodiencephalic modulation on small intestinal mucosa was studied in rats subjected to massive blood loss. Massive blood loss induced structural changes in intestinal epitheliocytes typical of tissue hypoxia: intraintestinal edema, mitochondrial alterations, and microcirculation disturbances. A single session of mesodiencephalic stimulation carried out 60 min after massive blood loss produced a pronounced adaptive effect, restored disturbed structure of the intestinal villi, and induced compensatory hypertrophy of the mitochondria. This suggests that mesodiencephalic modulation promotes normalization of intracellular energy metabolism, improves the absorption capacity of bordered enterocytes, and restores the disturbed intestinal barrier.

Adaptation, Physiological↗
PubMed1990

Second messenger-induced modulation of Thy-1 antigen expression: an ELISA study using murine thymic and neuroblastoma cells.

Freshly isolated cells of murine thymuses and in vitro cultured murine neuroblastoma cells were seeded into microplates, treated with either phorbol myristate acetate (PMA) or dibutyryl-cyclic adenosine monophosphate (DBcAMP), and then processed for enzyme immunoassay to analyze with G4 monoclonal antibody the expression of Thy-1.2 antigen epitope. Pretreatment of thymic cells with PMA resulted in little, if any, decrease of Thy-1 expression, while treatment of these cells with DBcAMP caused a significant down-modulation of the epitope. DBcAMP did not affect binding of another murine IgG antibody to the thymic cells. Modulation of the epitope on thymic cells caused by DBcAMP was dose-dependent with maximal effect seen at the drug concentration of 10(-4) M. However, at various doses of DBcAMP (10(-6) to 10(-3) M) we were unable to detect any significant shift of Thy-1 expression on neuroblastoma cells. Though mechanisms of the above phenomena need further elucidation, we conclude that cellular ELISA may provide a useful alternative to more commonly used cytofluorimetric studies for the analysis of immune cell-surface antigen expression and its pharmacological and physiological modulation.

Animals↗
PubMed1985

[2 calcium currents in the somatic membrane of neurons of Helix pomatia].

Two different calcium currents were revealed in the somatic membrane of Helix pomatia neurons. In addition to the main current described in literature, depolarizing the membrane from the holding potential level (-120 divided by -100 mV) an additional calcium current was observed. It was activated at depolarizations to -80 divided by -40 mV. Contrary to the main calcium current it did not deteriorate during intracellular perfusion by solutions containing fluoride. Time-dependence of this current could be described in the framework of the Hodgkin-Huxley model with time constants for activation and inactivation equal to tau m = 6-8 ms and tau h = 300-600 ms, respectively. The amplitude of this current increased with increase of extracellular Ca2+ concentration and decreased after addition of Co2+, Ni2+, Cd2+, nifedipine and verapamil. Dissociation constants of these substances with corresponding channels determined for the maximum of current-voltage relationship were 2 (Ca2+), 3 (Co2+), 0.06 (nifedipine) and 0.2 mmol/l (verapamil). Properties of the fluoride-insensitive calcium current and data obtained for other calcium channels are compared. Its possible functional role is also discussed.

Animals↗
PubMedDate not supplied

[Types of circulation in people with varying physical training].

In 125 young healthy males the cardiac output was estimated by means of tetrapolar rheoplethysmography. The marked differences were found in indices of central hemodynamics and specific peripheral resistance between the groups of sportsmen and non-sportsmen in spite of almost equal systemic arterial pressure in both groups. These differences are greatly due to the types of hemodynamics and in lesser degree--to the level of physical training and trend of sporting training.

Adult↗
PubMedDate not supplied

[Experience in treating logopedics patients by mesodiencephalic modulation].

Mesodiencephalic modulation (MDM), transcranial exposure of the middle brain structures to electric signal, selectively activates central regulatory systems. MDM has been introduced into the practice of the Center for Hearing and Speech Disorders. A course of treatment on the MDM-1 unit was performed in 665 patients. Irrespective of the symptoms and the disease, 6-7 procedures resulted in improvement. 5% of cases improved significantly 1-2 weeks after the end of the treatment, 17 patients (2.7%) required a repeated course 2-3 months after the initial treatment.

Adolescent↗
PubMedDate not supplied

[Effect of mesodiencephalic modulation on peripheral blood immunocompetent cells in viral-bacterial infections].

The effect of mesodiencephalic modulation (MDM) on peripheral blood immunocompetent cells during a mixed viral/bacterial infection was studied in 10 patients aged 15 to 35 suffering from acute respiratory diseases complicated by lacunar tonsillitis. Control group consisted of 10 patients aged 16 to 42. A course of MDM consisted of 4-5 daily 10-min sessions. MDM alleviated the course of respiratory diseases complicated by lacunar tonsillitis at the expense of activating body response to inflammation and due to immunomodulating effect of electropulse exposure on lymphocyte subpopulations.

Acute Disease↗