Second messenger-induced modulation of Thy-1 antigen expression: an ELISA study using murine thymic and neuroblastoma cells.

G V Pinchuk; L N Pinchuk; A V Grishchenko

doi:10.3109/08820139009041835

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Second messenger-induced modulation of Thy-1 antigen expression: an ELISA study using murine thymic and neuroblastoma cells.

G V Pinchuk·L N Pinchuk·A V Grishchenko

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Abstract

Freshly isolated cells of murine thymuses and in vitro cultured murine neuroblastoma cells were seeded into microplates, treated with either phorbol myristate acetate (PMA) or dibutyryl-cyclic adenosine monophosphate (DBcAMP), and then processed for enzyme immunoassay to analyze with G4 monoclonal antibody the expression of Thy-1.2 antigen epitope. Pretreatment of thymic cells with PMA resulted in little, if any, decrease of Thy-1 expression, while treatment of these cells with DBcAMP caused a significant down-modulation of the epitope. DBcAMP did not affect binding of another murine IgG antibody to the thymic cells. Modulation of the epitope on thymic cells caused by DBcAMP was dose-dependent with maximal effect seen at the drug concentration of 10(-4) M. However, at various doses of DBcAMP (10(-6) to 10(-3) M) we were unable to detect any significant shift of Thy-1 expression on neuroblastoma cells. Though mechanisms of the above phenomena need further elucidation, we conclude that cellular ELISA may provide a useful alternative to more commonly used cytofluorimetric studies for the analysis of immune cell-surface antigen expression and its pharmacological and physiological modulation.

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Animals Antigens, Surface Bucladesine Down-Regulation Enzyme-Linked Immunosorbent Assay In Vitro Techniques Mice Mice, Inbred CBA Neuroblastoma Second Messenger Systems Tetradecanoylphorbol Acetate Thy-1 Antigens Thymus Gland Journal Article

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G V Pinchuk, L N Pinchuk, A V Grishchenko. 1990. Second messenger-induced modulation of Thy-1 antigen expression: an ELISA study using murine thymic and neuroblastoma cells.. https://doi.org/10.3109/08820139009041835

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