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Biomedical subjects

A V Kuznetsova

Publications and source records attributed to A V Kuznetsova.

At least 19 recordsLinked to original sources
PubMed2006

[Prognosis of remote consequences of combat mental trauma using logical statistical methods].

Prognosis of remote consequences of acute stress disorders and adaptation disorders as posttraumatic stress disorders (PTSD) is possible using relevant mathematical methods. The authors have used the following logical statistical methods: algorithms for search and statistical verification of optimal fragmentation of variables in multi-dimensional space ("Q-nearest neighbors", "statistically weighed syndromes") as well as a program of searching for multiple conjunctions of variables that allowed to single out statistically significant combinations of variables (p<0,001) and to divide the sample into two groups: with favorable and with unfavorable outcome both at the acute and remote stages of mental trauma. Predictors of unfavorable outcome found in the acute period of mental trauma were as follows: affective tension, impulsivity, high personal anxiety and depressive tendencies with reduced activity. Comparing to clinical evaluation, subjective criteria of mental state (self-rating) retained its informativeness for prognosis of protracted PTSD types. Using this set of variables, it became possible to predictt remote consequences (2-4 years) of psychogenias of combat situation with reliability of recognition of 85% for the favorable outcome and 79% for cases of protracted types.

Adolescent↗
PubMed2006

[The long-term results of coronary artery bypass grafting in patients with type II diabetes].

A three-year follow-up of patients with coronary heart disease (CHD) and type II diabetes after coronary artery bypass grafting, shows that the following pathologic conditions are significantly more frequent: arterial hypertension, visceral obesity, marked disturbances of blood lipid spectrum, an increases CHD duration, and an increased rate of myocardial reinfarction and revascularizations. The study shows that the presence of diabetes mellitus in CHD patients undergoing coronary artery bypass grafting, is associated with pronounced disturbances in blood lipid spectrum, and is an important risk factor of coronary event progression.

Coronary Artery Bypass↗
PubMed2005

[Antiischemic and metabolic effects of nebivolol and metaprolol CR/XL (betalok ZOK) in patients with postinfarction heart dysfunction].

The purpose of the study was to evaluate anti-ischemic and metabolic effects of the cardioselective beta-adrenoblockers nebivolol and retarded metoprolol-metaprolol CR/XL (betalok ZOK) in patients with postinfarction heart dysfunction, associated with type II diabetes mellitus (DM). 40 patients with coronary heart disease (CHD), functional class (FC) II-III exertional angina, postinfarction left ventricular (LV) dysfunction, and NYHA FC II heart failure, associated with type II DM, were randomized into 2 groups. The 20 patients of the 1st group were administered nebivolol in a dose of 1.25 to 5 mg per day, the 20 patients of the 2nd group - betalok ZOK in a dose of 12.5 to 100 mg per day. The course therapy lasted 8 weeks. The effects of the treatment were evaluated using paired veloergometry, echoCG, and lipid spectrum analysis. The study found that nebivolol in a mean dose of 4.2 +/- 0.3 mg per day and betalok ZOK in a dose of 46.5 +/- 6.2 mg per day reduced the frequency and severety of angina attacks (by 73.8% and 67.8%, respectively) and daily nitroglycerine uptake (by 78.6% and 69.1%, respectively), and increased activity tolerance (by 7.9% and 25.3%, respectively). None of the preparations displayed any adverse effects on carbohydrate exchange and blood lipid spectrum. Nebivolol, unlike betalok ZOK, significantly (p = 0.02) reduced triglyceride blood level by 29%. Thus, the new generation cardioselective beta1-adrenoblockers nebivolol and metoprolol CR/XL (betalok ZOK) provide anti-ischemic and metabolic effects in patients with CHD and postinfarction LV dysfunction, associated with type 2 diabetes mellitus. Nebivolol is preferable as far as blood lipid spectrum is concerned.

Adrenergic beta-Antagonists↗
PubMed2005

[The role of clinical and imaging criteria in risk assessment during preoperative chemotherapy for osteosarcoma].

Prognosis for stage IIB osteosarcoma was evaluated versus the role of preoperative management of clinico-radiologic status in 293 patients. Three--five preoperative intra-arterial cycles of doxorubicin 90mg/m2 or cisplatin 120mg/m2 were given in 1986-1999, and later were followed by 3-4 cycles of doxorubicin and cisplatin in similar doses. Clinico-radiologic status was assessed in the course of preoperative chemotherapy. One hundred fifty patients were alive at the last examination, 139 had died of tumor progression, and 4 - chemotherapy complication. The two courses of preoperative chemotherapy were followed by more favorable outcome. Complete clinical response, tumors downsized to 300 mm, intra-osseous healing, periosteal assimilation and margination of extra-osseous masses had a significant positive impact on disease-free survival in the chemotherapy group. The data were used for the developing of models for individual risk evaluation and prognosis. Clinico-radiologic status monitoring in the course of preoperative chemotherapy is instrumental in predicting risks as well as designing alternative strategies of treatment at earlier stages.

Adult↗
PubMed2002

[Optimization of conditions for isolating chromosomes in flow sorting].

Factors for purity and efficiency of flow chromosome sorting were analysed on the base of quantitative analysis. The sorting rate and relative purity of individual chromosome fractions are determined both by the quality of initial chromosome suspension, instrument parameters and gate position on experimental histograms. The described procedure of analysing sorting efficiency and fraction purity allowed to formulate general tips for optimization of sorting conditions depending on a given strategy: maximization of quantity of obtained material, or achieving maximum purity for sorted fraction. The analysis is carried out on the bases of chromosome distribution parameters: their relative halfwidths and distances. These parameters can be obtained by the quantitative analysis programs for flow cytometry data. It is shown that the critical parameter for sorting purity is the level of contaminated objects in a zone of sorted chromosome signal registration. In addition, the fraction purity depends on the cover extent between different chromosome distributions. Created procedure allows to build up nomograms linking the sorting efficiency with fraction purity, depending on the position of sorting gates. These monograms permit to determine the position of sorting gates in relation to one or another strategy: 1) maximum rate for chromosome material obtaining, 2) maximum fraction purity, or 3) compromise between the two. The presented analysis allows to optimize the chromosome sorting process for subsequent genome investigations providing chromosome material with controlled characteristics.

Chromosomes, Human↗
PubMed1998

Taraxalisin -- a serine proteinase from dandelion Taraxacum officinale Webb s.l.

Latex of dandelion roots contains a serine proteinase that hydrolyzes a chromogenic peptide substrate Glp-Ala-Ala-Leu-pNA optimally at pH 8.0. Maximal activity of the proteinase in the roots is attained in April, at the beginning of plant development after the winter period. The protease was isolated by ammonium sulfate precipitation of the root extract followed by affinity chromatography on a Sepharose-Ala-Ala-Leu-mrp and gel filtration on Superose 6R performed in FPLC regime. Pure serine proteinase named taraxalisin was inactivated by specific inhibitors of serine proteinases, diisopropylfluorophosphate (DFP) and phenylmethylsulfonylfluoride (PMSF). Its molecular mass is 67 kDa and pI 4.5. pH stability range is 6-9 in the presence of 2 mM Ca2+, temperature optimum is at 40 degrees C; Km=0.37+/-0.06 mM. The substrate specificity of taraxalisin towards synthetic peptides and insulin B-chain is comparable with that of two other subtilisin-like serine proteinases, cucumisin and macluralisin. The taraxalisin N-terminal sequence traced for 15 residues revealed 40% coinciding residues when aligned with that of subtilisin Carlsberg.

Amino Acid Sequence↗
PubMed1997

[Affinity sorbents with tripeptide morpholine ligands for isolation of proteases].

New affine sorbents were synthesized involving tripeptide morpholides H-Ala-Ala-Leu-Mrp and H-D-Ala-Leu-Arg-Mrp as ligands that mimic substrates of subtilisin-like proteases and kallikrein, respectively. These were used for the isolation and purification of several proteases: trypsin, pepsin, alpha-chymotrypsin, thrombin, kallikrein, and termitase and were also efficient in the isolation of proteolytic enzymes from complex mixtures, such as the urine of children suffering from glomerulonephritis, hepatopancreas of Kamchatka crab, and dandelion roots. The ligands are competitive inhibitors of a number of proteases, and therefore, they were supposed to interact with the substrate binding sites in these enzymes.

Animals↗
PubMed1992

[Neurohistological characteristics of regeneration of the ends of the injured nerve under measured traction].

A resection of sciatic nerve in 25 dogs was performed out and the ends of cut nerve were connected by sutures with special apparatus for quantified traction of nerve stumps. 5 days after resection traction was stented at the rate of 0.25 mm twice a day. A histological analysis performed in 5, 12, 19, 26, 33 days after resection an 2-12 months after nerve suture has shown that stress strain effects on intercalation of nerve structures (Ilizarov's effect). Newly formed Schwann chains free of products of axonal and myelin disruption have got a perfect longitudinal orientation and that's why can be considered as an optimum substratum for axonal regeneration.

Animals↗
PubMed1989

[Detection of antibodies to Rickettsia prowazekii using 3 serologic tests].

Commercial antigens of R. prowazekii may be used for the diagnosis of the typhus group rickettsiosis by the new solid-phase indirect enzyme immunoassay (SPI EIA). High sensitivity of the test makes the diagnosis of rickettsioses more effective and ensures an earlier detection of typhus patients. The authors have employed SPI EIA modification with polyethylene glycol, permitting a change in the incubation regimen (20 degrees C instead of 37 degrees C) and more rapid reaction; this recommends the method for laboratory and field practice. The possibility of a visual assessment of the test results also recommends it for wide practice.

Antibodies, Bacterial↗
PubMed1989

[An indirect solid-phase immunoenzyme method in diagnosing rickettsial infections].

Sera of Brill-Zinsser disease patients (70 samples) Q fever patients (12 samples) and healthy persons from Q fever enzootic areas (99 samples) were studied in indirect solid phase enzyme immunoassay (ELISA), indirect immunofluorescent antibody test (IFA) and indirect passive hemahglutination test. ELISA and IFA have been proved as most sensitive tests for detection of rickettsial antibodies in both clinical and retrospective diagnosis of Rickettsial diseases.

Antibodies, Bacterial↗