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A von Graevenitz

Publications and source records attributed to A von Graevenitz.

At least 73 records · Page 4Linked to original sources
PubMed1987

[Pleural empyema due to Streptococcus milleri].

Thus far little attention has been focused on the precise identification of Streptococcus viridans to species level and the clinical relevance of Streptococcus milleri, yet there is growing evidence that closer identification would be of significant clinical value. Streptococcus milleri was identified in 142 isolates from approximately 120 patients over a period of 12 months (June 1984-May 1985) at the Institute of Medical Microbiology, Zurich University Hospital. Pleural empyema was the source in eleven patients (10%). The presentation of six cases of pleural empyema in which Streptococcus milleri played a role, together with a review of the literature, may serve to emphasize the importance of an organism whose taxonomic status is still unclear.

Adult↗
PubMed1987

[Quantitative sensitivity determination of bacteria. Indications and methods].

The authors describe the indications for quantitative susceptibility tests and also the methods themselves (agar dilution, macrodilution, combination tests, tests for beta-lactamase production). This study forms part of a series on susceptibility methods which started with a paper on disk susceptibility testing (this journal, 1984; 114: 1079-1086).

Anti-Bacterial Agents↗
PubMed1987

Two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures.

A miniaturized 2-h system for detection of lactose-negative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. The system was challenged with 210 strains of lactose-negative and lactose-positive species grown on MacConkey agar. Specific and constant patterns were found for Salmonella, Shigella A-C, Yersinia enterocolitica, Aeromonas spp. and Vibrio cholerae. Shigella sonnei and Hafnia alvei had an identical pattern, likewise Plesiomonas shigelloides, halophilic vibrios and Pseudomonas aeruginosa.

Bacteria↗
PubMed1987

Reliability of the colistin disk test in identification of Serratia marcescens and Serratia liquefaciens.

Resistance to polymycin B or E (colistin) in the disk test, which is used as a means of identification, was tested in 43 strains of Serratia marcescens and 26 of Serratia liquefaciens. While all strains had MIC values greater than 32 mg/l for colistin, false susceptibility to both polymyxins was encountered in the 24 h disk test in 9% of Serratia marcescens and 12-96% of Serratia liquefaciens strains, depending on the kind of polymyxin, temperature and duration of incubation. Using colistin disks and incubation at 25 degrees C for 48 h, the percentage of false susceptible results could be minimized.

Colistin↗
PubMed1987

Evaluation of a small "conventional" identification system for fermentative gramnegative rods using a computerized data base.

A small "conventional" identification system consisting of six tubed media totalling eleven reactions was used to identify 467 strains of Enterobacteriaceae, 15 strains of Aeromonas sp., and 3 strains of Plesiomonas shigelloides. The system identified 371 strains (76.5%) correctly to maximum identification (species or genus) level and an additional 13 strains (2.7%) to genus level where species level identification would have been possible. Ninety strains (18.6%) were not identified. Only 11 strains (2.3%) were misidentified. The system is inexpensive compared with commercial systems and can be used for primary identification of fermentative gramnegative bacteria in a clinical microbiology laboratory. It comes with a computerized data base in the form of an identification manual.

Aeromonas↗
PubMed1987

Fleroxacin (Ro 23-6240): activity in vitro against 355 enteropathogenic and non-fermentative gram-negative bacilli and Legionella pneumophila.

The antibacterial activity of fleroxacin (Ro 23-6240, AM-833), a new 6-fluoroquinolone, was determined against 149 strains of enteropathogenic bacteria (17 species) and 191 strains (28 species) of glucose non-fermentative Gram-negative rods (excluding Pseudomonas aeruginosa), and against 15 strains of Legionella pneumophila. The cumulative susceptibility of these groups of bacteria to Ro 23-6240 at the 2 mg/l level were 99.2%, 80.1 and 100% of tested strains, respectively.

Anti-Bacterial Agents↗
PubMed1985

Evaluation of pertinent parameters of a new identification system for non-enteric gram-negative rods.

The API 20 NE (Rapid NFT) system for identification of non-enteric aerobic gram-negative rods was evaluated using 431 strains of species covered by the analytical profile index and previously identified by conventional methods. In an analysis of several parameters, 343 (80%) of the strains were identified correctly with good to excellent probabilities of positive reactions (80.0-99.9%), 190 (55%) of these within 24 h; 74 (17%) yielded primary identifications with lower probabilities. Only 3% of the strains could not be correctly identified. The system is very satisfactory for routine use, but could be improved by modified pre-testing of the strains and by including more species in the profile index.

Bacteriological Techniques↗
PubMed1985

Resistance patterns of nonfermentative gram-negative rods and aeromonads to beta-lactam antibiotics: a diagnostic aid.

Consistent resistance to certain antimicrobial agents has in the past been of help in the diagnosis of nonfermentative gram-negative rods. In order to establish patterns of consistent resistance to 15 mostly newer betalactam antibiotics, 561 strains of such organisms plus Aeromonas and Plesiomonas were tested with the Kirby-Bauer method and also against the selective agent C-390 in three concentrations in Mueller-Hinton agar. No species-specific patterns emerged but the resistance data can be used to exclude certain species. C-390 was almost specifically selective for Pseudomonas aeruginosa.

Acridines↗
PubMed1985

Growth of Aeromonas spp. on cefsulodin-Irgasan-novobiocin agar selective for Yersinia enterocolitica.

Twenty-eight strains of Aeromonas spp. were analyzed for their ability to grow on two different kinds of cefsulodin-Irgasan (triclosan; Ciba-Geigy AG, Basel, Switzerland)-novobiocin (CIN) agar containing 15 or 4 mg of cefsulodin per ml and on inositol-bile salts-brilliant green (IBB) agar. Relative to blood agar, 68% of the strains were inhibited by more than 2 logs (i.e., less than 1% growth) at 37 degrees C (39% at 25 degrees C) on CIN I (high cefsulodin concentration), 7% were inhibited at either temperature on CIN II (low cefsulodin concentration), 4% were inhibited on IBB agar at 37 degrees C, and none were inhibited on IBB agar at 25 degrees C. These results reflect the MICs of cefsulodin on CIN Base: the MIC for 50% of the strains was 8 mg/liter at 37 and 25 degrees C, and the MICs for 90% of the strains were 16 mg/liter at 37 degrees C and 64 mg/liter at 25 degrees C. The MICs of Irgasan and novobiocin were far beyond the concentrations used in CIN media. We argue that CIN agar containing 4 mg of cefsulodin per ml (CIN II) can be used for the simultaneous detection of Aeromonas spp. and Yersinia spp.

Aeromonas↗
PubMed1984

[Standardized disk tests for the determination of bacterial resistance ].

This paper describes the standardized Kirby-Bauer disc method for the determination of antibacterial susceptibility, also including choice of antimicrobials, interpretation of results, problems with particular bacteria, and quality control. Only a meticulous adherence to the details of the method will guarantee reliable results.

Aminoglycosides↗
PubMed1984

Adult vertebral osteomyelitis caused by Haemophilus influenzae.

Two cases of adult vertebral osteomyelitis with typical symptoms are reported. Haemophilus influenzae serotypes b and c, respectively, were isolated from needle biopsies of the vertebrae. Both patients recovered with antimicrobial treatment, immobilization, and subsequent physiotherapy. No source or predisposing factor for the infection could be found. Only one similar case has been reported in the literature.

Adult↗