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Biomedical subjects

B Błaszczyk

Publications and source records attributed to B Błaszczyk.

At least 55 records · Page 3Linked to original sources
PubMed1984

Experimental allergic encephalomyelitis in the chicken. Class-specific antibody to myelin basic protein, the ability of T cells to cause GvH reaction, brain and spleen histology, and nonspecific biochemical indicators of inflammation.

Experimental allergic encephalomyelitis (EAE) was induced in chickens by injecting human myelin basic protein (MBP) in Freund's complete adjuvant (CFA). Antibodies of IgM, IgA and IgG class to MBP were measured by enzyme-linked immunosorbent assay (ELISA). The ability of the blood from the experimental chickens to induce graft-versus-host (GvH) reaction was tested in chick embryos. Serum concentrations of sialic acid and antitrypsin were monitored. Substantial inflammatory infiltrations were found in various parts of the central nervous system of the animals injected with MBP, but not in the controls receiving plain CFA. Enlargement of the white pulp and plasmocytosis were found in the spleens of the EAE animals. In the course of the disease, anti-MBP IgM and IgA antibody levels strongly increased, while IgG antibodies remained unchanged. The GvH caused by the blood from EAE animals did not differ from that of control birds. The EAE group showed significantly higher antitrypsin concentration than the controls. The increase in sialic acid concentration was of equal magnitude in the EAE animals and in the CFA controls.

Animals↗
PubMed1984

Induction of tolerance in chickens by low doses of human serum albumin administered after hatching.

The possibility was tested whether tolerance can be induced in newly hatched chickens by a single injection of HSA on the day of hatching in doses one or several orders lower than the dose employed in previous experiments (100 mg of HSA). Doses of 10 and 1 mg of HSA induced a statistically significant suppression of anti-HSA antibody formation after challenge at the age of 2 and 4 weeks. Injection of 0.1 mg of HSA into newly hatched chickens also produced a statistically significant decrease in antibody formation after challenge at 2 weeks of age.

Animals↗
PubMed1983

Immunosuppressive properties of yeast extract.

Autolysate of yeast extract exhibits immunosuppressive properties. It inhibits phytohemagglutinin stimulated incorporation of 13C thymidine to rabbit lymphocytes. When administered to mice after immunization with sheep erythrocytes, it suppresses the appearance in the spleen of IgM antibody-producing cells (PFC). It also prolongs the survival time of skin allotransplants in mouse if given before and after transplantation.

Actinomyces↗
PubMed1983

Morphological and biochemical studies on experimental allergic encephalomyelitis in inbred lines of chickens.

An attempt to establish the relation between morphological and biochemical indices determining the inflammatory state in brain after induction of allergic encephalomyelitis in chickens was taken up. Symptoms of encephalomyelitis were accompanied by the changes within T and B lymphocyte population and by distinct increase of activity of trypsin inhibitor and content of sialic acid in blood serum.

Animals↗
PubMed1983

Chemical, pharmacological and oncostatic properties of 5-(4'-hydroxybenzylidenoimino)-4, 6-diketo-4, 5, 6, 7-tetrahydropyrimidine-[4, 5-d]-3-methyl-isothiazole (compound IP-10).

Advanced preclinical studies on IP-10 preparation (4,6-diketo-4, 5, 6, 7-tetrahydropyrimidine-[4, 5-d]-3-methyl-isothiazole) were carried out. The drug was shown to be devoid of the irritating local effect, mildly toxic and hardly absorbing when administered per os. The toxic effect showed tendency toward cumulation. In the long-term exposure it did not affect either the elements of peripheral blood or parenchymatous organs. It exerted slight hypotensive effect on the circulatory system but only after intravenous administration. In relation to the smooth muscle organs and central nervous system, IP-10 was only slightly active. Weak effect of the compound was observed with bacteria and fungi. In the case of transplantable tumors, its activity was differentiated. It exerted a significant effect in relation to leukemia, melanoma B-16, Ehrlich carcinoma and Nemeth-Kellner lymphoma. As other isothiasole derivatives, IP-10 exhibits an interesting pharmacological, easy to render activity; particular attention should be paid to its oncostatis activity.

Animals↗
PubMed1982

[Barbital buffer in tablets as a solvent for complement fixation test (CFT)].

The purpose of this paper was to develop a tableting technology of barbital buffer as a standard diluent easy in use for complement fixation test (CFT). As starting point, barbital (veronal) buffer after Alton et al. [1] was assumed. Among 4 solubilizers polyvinyl pyrrolidone (PVP 10 000) was chosen, which provided a good solubility of the barbital in distilled water at 20 degrees C and it affected favourably the complement activity (C'). PVP 10 000 added to the components of barbital buffer at an amount of 0.275 per 10 tablets, in the form of solution for granulation, provided the required physical properties of the tablets. As compared with the barbital buffer after Alton et al. [1], the tablets produced affected favourably C' activity, decreased the range of spontaneous haemocyte lysis, and they did not effect CFT titers of two anti-Brucella abortus standard sera as well as 77 sera of cattle infected with brucellosis under natural conditions. The described physical and biological properties of the barbital buffer tablets with PVP addition indicate their usability as diluent for CFT. A standard diluent used in the form of tablets in all Poland's laboratories should provide a better reproducibility of CFT results in diagnostic examinations towards brucellosis as well as other infectious diseases in men and animals.

Animals↗
PubMed1981

Chemical and biological properties of 2-pyridyl-benzyl-carbinol.

Three isomers of pyridylbenzylcarbinol were synthetized and their chemical properties and biological activity were studied. Clear dependence between chemical structure and antitumor activity was observed. As expected most active appeared 2-pyridylbenzylcarbinol (alpha-carbinol). It showed significant antitumor activity against subcutaneous tumors of Ehrlich carcinoma. Nemeth-Kellner lymphoma and sarcoma 180. On a chronic treatment schedule tumor inhibition from 60 to 80% was induced. No activity was seen in L1210 and P388 models. In B16 melanoma the significant inhibition of tumor growth was obtained at 200 mg/kg/injection. Tumor inhibitory effect was also observed in Yoshida sarcoma. Depending on a dose and schedule the increase in lifespan from 45 to 147% was achieved. LD50 and MTD were determined and were 750 +/- 87.65 mg/kg and 557.8 mg/kg, respectively. Only slight suppressive effect of alpha-carbinol on immunological system was showed. It concerned mainly immunological reactions with cellular system involved, in which distinct suppressive effects were noted. Specific and nonspecific humoral reactivity was little or not at all affected by alpha-carbinol.

Animals↗
PubMed1980

Preclinical pharmacologic investigations on a new group of acridine derivatives with oncostatic activity. I. Acute and subchronic action.

The influence of l-nitro-9-/2-dihydroxyethylamino-ethylamino-/-acridine (C-835) and l-nitro-9/3-izopropylaminopropylamino/-acridine (C-846) on the basic functions of animal organism in acute and subchronic experiments was studied. Both compounds displayed distinct all-biologic activity. Their toxicity (LD50) in mice was contained in milligramme interval (i.v.), while LD50 administered into the stomach was several dozen times higher. Even administration in protective phosphate buffer, which provides the most neutral pH, produced quite strong local irritant action. The range between LD50 and LD10 was relatively small, which proved relatively small tolerance factor. Both preparations injected intravenously in the doses starting from several mg/kg had hypotensic influence due to their affinity with the myocardium, the parasymphatetic system and the vascular system. The preparations acted spasmolytically on intestine muscles both in vivo and in vitro in a number of animal species. Central action showed clear stimulating component in a behavioric test. The effect of the compounds in interaction to hypnotic and convulsant drugs was equivocal and dependent on the dose and the reference-preparation. The examined compounds did not influence reproduction processes and did not display the teratogenic action. They impaired only the speed of growth of the newborns. The effect of both examined compounds was qualitatively and quantitatively similar.

Aminacrine↗
PubMed1980

Preclinical pharmacologic investigations on a new group of acridine derivatives with oncostatic activity. II. Chronic action.

Influence of 1-nitro-9-(2-dihydroxyethylaminoethylamino)-acridines (C-835) and 1-nitro-9-(3-isopropylamino-propylamino)-acridines (C-846) on animal organs and their basic functions was studied in the chronic action. Rabbits responded to the drugs with considerable tolerance which was less manifested in rats. Slight toxic effect was observed only after maximal doses with which the animals survived 3-month exposure. Enzyme studies and creatinine clearance test did not show any clear-cut impairment of hepatic and renal function. Neither did the preparations influence the red and white blood system, nor the blood clotting time. Microscopic examinations showed their slight (initially) morphologic changes which later passed into degenerative changes (liver, kidney, myocardium) as well as typical of oncostatics, changes in intestinal villi, nuclei and lymphatic system. Also desquamation of intestinal epithelium, hypertrophy of their lamina propria, impairment of spermato- and spermiogenesis as well as atrophy of lymphatic germinal centers and decreased number of small lymphocytes in lymph nodes and spleen were noted. Deviation between the results of function and morphologic tests were discussed. Microscopic changes observed after treatment of the tested acridine derivatives were considered too small to damage the particular organs function.

Aminacrine↗
PubMed1980

Pharmacological studies on p-chlorophenylamide of 3-methyl-5-benzoylaminoizothiazole-4-carboxylic acid (compound ITCL) the preparation of eventual anti-inflammatory effect.

Pharmacological activity of p-chlorophenylamide of 3-methyl-5-benzoylaminoizothiazole-4-carboxylic acid (compound ITCl) was studied with special regard to its anti-inflammatory effect. Compound ITCl is moderately toxic with no distinct circulatory and central effect. On the other hand, it shows strong anti-inflammatory activity in many tests of immunologically specific, nonspecific and infectious inflammations. The greatest efficacy of the compound was noted in the therapy of experimental, immunologically non-specific (xylene, carrageenin and cotton-test) inflammations. Weaker and less regular therapeutic effect was noted in the cases of immunologic inflammations -- mainly in contact reaction to dinitrochlorobenzene. Compound ITCl was ineffective in relation to transplantation barrier. Therapeutic efficacy of the compound was not observed in herpetic inflammation of the skin. On the other hand, a promising therapeutic effect was noticed in relation to cow-pox virus keratitis. Compound ITCl is well tolerated. It shows no toxic effect on white and red blood cells system in a 4-week exposition. Compound ITCl stabilizes cell membranes. Practical aspect of the obtained results was discussed.

Animals↗
PubMed1979

Pharmacological studies on new oncostatic acridine derivatives. I. Acute and subchronic action.

Preclinical pharmacological studies of two acridine derivatives, dihydrochloride N10-oxide 1-nitro-9-/3-dimethylaminopropylamino/-acridine (C-666) and dihydrochloride 1-nitro-9-[(2-dimethylamino)-1-methylethylamino]-acridine (C-829) are reported. Both compounds are characterized by biological activity, poor absorption from the gastrointestinal tract and local irritant action. Quality differences in an effect of both investigated acridine derivatives on the central nervous system were noted. C-666 proved to be deprived of the effect typical of the central component compounds while C-829 demonstrated mostly sedative activity. Clear dissociation in the effect of these both compounds was seen in in vitro experiments on isolated smooth muscle organs. C-666 acted spasmolytically on the motory action of intestine muscles while C-829 acted spastically. Both preparations had clearly hipotensic influence which can be due to the vascular effect and to their affinity with the intramyocardium transmitting system. Neither a distinctive effect on reproductivity of animals nor the teratogenic action were observed in the functional experiments.

Acridines↗
PubMed1979

Pharmacological studies on new oncostatic acridine derivatives. II. Chronic action.

Encouraging results of preclinical pharmacologic analysis prompted the following study on the chronic action of N10-oxide 1-nitro-9-/3-dimethylaminopropylamino/-acridine (C-666) and 1-nitro-9- [/2-dimethylamino/ -1-methylethylamino] -acridine (C-829). Toxic influence of both compounds on liver, kidneys, gonads and intestine epithelium was observed. These histological changes were not, however, followed by any disorders in functions of liver and gonads. Only administration of compound C-666 was followed by the decrease of filtration of renals glomuses. A slight involutional influence of acridine derivatives on lymphopoetic reproductive center of thymus and lymph nodes was observed. At the same time there were no disorders in the value of circular leukocyte system. It was stated that the investigated compounds had no effect on the system of the red cells of the blood; only blood clotting time was prolonged. Compounds C-666 and C-829 did not inhibit the growth of tested animals.

Aminoacridines↗