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Biomedical subjects

B Bercovici

Publications and source records attributed to B Bercovici.

44 records · Page 3Linked to original sources

The cytology of the human peritoneal fluid.

A series of 19 human peritoneal fluids obtained by aspiration at laparoscopy and cul-de-sac punctures were analysed for their cellular content. Four types of cells were differentiated: macrophages, mesothelial cells, lymphocytes and polymorphonuclear leukocytes. The macrophages and mesothelial cells represented the two major types of cells, each group constituiting about 36 per cent of the population, while the lymphocytes and polymorphonuclears represented 18 and 7 per cent of the population, respectively. Macrophages were identified by their capacity to engulf alcohol-killed Staphylococcus albus. It appears that by this method of macrophage identification, a more accurate representation of the cellular content of peritoneal fluid can be obtained.

Ascitic Fluid↗

The cytology of the retained IUD with relation to the mechanism of action.

Cellular reactions to 82 IUDs retained for different periods of time have been examined using the "loopal smear" method. The smears were divided according to periods of retention and degree of inflammatory reaction. In 47 cases the inflammatory reaction was graded as moderate to severe, while in 35 cases the reaction was graded as mild. Since contraception was effective also in those cases presenting with mild reactions, it must be concluded that factors other than inflammation play a role in the contraceptive mechanism. Cells obtained from 15 IUD washings were incubated with Staphylococcus albus in order to identify macrophages. In none of these cases were macrophages identified, thus rendering questionable the hypothesis that the contraceptive effect of the IUD is based on phagocytosis of spermatozoa.

Endometrium↗

The cytology of vaginal, cervical and endometrial smears obtained at the time of embryo transfer during in vitro fertilization.

Seventy-four women enrolled in an in vitro fertilization (IVF) program had cytologic smears of the vagina, cervix and endometrium obtained at the time of embryo transfer (ET). Of these, 68 vaginal, 46 cervical and 25 endometrial smears were available for cytologic examination. Of the 68 vaginal smears, 4% showed a proliferative pattern, 40% were early secretory and 56% were advanced secretory. The 46 cervical smears demonstrated a delayed hormonal effect, with 70% showing a proliferative pattern, 23% early secretory and 7% advanced secretory cytology. Endometrial cells were obtained only when the Jones catheter, which has a side opening, was used. Twenty-two patients had both vaginal smears and suitable endometrial smears. Of these, 8 of the 9 patients with early secretory vaginal cytology had secretory endometrium while 10 of the 12 patients with mid-secretory vaginal cytology had secretory endometrium. The value of endometrial cytology in predicting conception following IVF-ET is unknown. It seems, however, that a good correlation exists between endometrial and vaginal cytology and that the latter may be of value as an additional, noninvasive tool for the evaluation of endometrial development.

Adult↗

Vaginal and oral cytology of the menopause. A comparative study.

A comparative cytologic study was made of the hormonal content of the vaginal and oral mucosa of menopausal women. Twenty-three women between one and ten years (early menopause) and 33 patients more than ten years (late menopause) after cessation of menstruation had oral and vaginal smears taken. Comparable smears from 21 young women, and oral smears from 18 males served as controls. The smears were evaluated by the maturation value method, and averages were established for each group. The vaginal smears had low maturation values of 40 and 22 in the early and late menopausal groups, respectively. In these women, the buccal smears' maturation values were high, above 70. Similar high maturation values were found in buccal smears of the young women and men. It was concluded that the high maturation values found in the oral smears of the menopausal women are not the result of hormonal effect but that of local mechanisms or irritative factors.

Adult↗

Vaginal and urinary cytology and blood hormonal examinations in patients with premature rupture of membranes.

Vaginal smears and urinary sediments of 29 pregnant women in their third trimester with premature rupture of membranes (PRM) were examined. Plasma radioimmunoassays (RIA) for estriol, human chorionic gonadotropin, human placental lactogen and progesterone were performed. Twenty-three normal pregnant women in the third trimester, serving as controls, underwent the same examinations. The vaginal smears and urinary sediments were evaluated as reflecting disturbed, slightly disturbed and normal placental function. Good correlation was found between the vaginal smears showing the typical and suggestive aspects of PRM and the placental function reflected in the same smears and the respective urinary sediments. From the hormonal examinations performed, the plasma RIA for progesterone presented significantly lower results in the PRM cases than in the control group. The results obtained suggest a lowering of the placental function in cases with PRM as reflected in cytologic and hormonal examinations.

Chorionic Gonadotropin↗